... and other related resources. Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring...by @drmellivora, an excellent blog post about workplace bullying featured on the always interesting Tenure...thing to do is not go into their labs in the first place. The good news There are many excellent mentors ...

... which studies immunity and the host-pathogen interface, previously developed a high-throughput neutralization...the virus to gain entry into human cells via human ACE2 receptors. In their assay, the pseudoviruses express...reporter which is used as a readout for infection of ACE2-expressing target cells. Any neutralized pseudoviruses...

...tech companies using only laptops and some server space. But what if you want to be an entrepreneur in ... graduate students with innovative ideas in all facets of the entrepreneurial process in order to develop...you start at a disadvantage in the health science space if you don’t have an advanced degree behind your...

...about how to solve this problem. I didn’t want to face another group of sleepy students at 8 a.m. many ...format I decided on was to have two teams of 4 people face each other on a single chosen clinical disease case...they agreed on each element to use, they had to place the selected card on the board game. They had 35...

... that cell. Since there is a limited amount of surface area on each cell, the way to get more AAV in contact...expression. How do you achieve high titers in the first place? Some AAVs are known to be difficult to produce ...concentration filter column, make sure to rinse off the surface of the filter membrane with the surrounding solution...

...where the mutation can be placed. With other techniques, when mutations are spaced farther apart, it becomes... which is freely available online. Its simple interface can guide you through both experimental and primer...

...solution thawed, as the CO2 was confined to the tube headspace when the samples were frozen. This was clear by...the gradual change in pH visible at the sample interface upon thaw (Fig. 2). Before thawing, all the samples...tubes prior to thaw released the CO2 from the headspace, thus mitigating the pH effects of dry ice storage...

...The most commonly used yeast by biotech and pharmaceutical companies is K. phaffii (formerly known as ...the system. Since then, multiple biotech and pharmaceutical companies (along with some research laboratories...the expression of recombinant proteins by the pharmaceutical and biotech industries as a protein expression...

... limited by the need for a specific PAM (protospacer adjacent motif) in order to bind the target sites...mediated a C to T conversion in the target sites adjacent to an NG PAM in human cells. The increased versatility...

...therapeutic proteins. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft...complexity of antigen processing events that take place within an antigen-presenting cell like a T cell....

...targeted known residues on the outer surface to break dimerization interfaces (affecting many of the RFPs developed...discovered reprogramming discrepancies, which they traced to a 9 amino acid N-terminal variation in the Klf4...genes identified as non-essential. The knockouts replace entire protein-coding regions (excluding start ...

...HEPN-independent. These enzymes also do not require a protospacer flanking sequence, so you can target virtually...either an unprocessed or processed gRNA array (22 nt spacer with 30 nt direct repeat) mediating potent knockdown...dementia-associated tau, delivering dCasRx and a 3-spacer array targeting the MAPT exon 10 splice acceptor...

...substrate, they synthesized diacetyl coelenterazine-h. Unlike coelenterazine-h, diacetyl coelenterazine-h does... to the media. Cellular esterase then converts diacetyl coelenterazine-h to coelenterazine-h, producing...

...cell!)—ATP. There are biosensors for intracellular, extracellular, and even the ADP:ATP ratio. Most of... from dopamine to glutamate, all the way to acetylcholine. These sensors have been tested in vivo, in ...

...genetic backgrounds. The standard RNAi vector, L4440, places a target gene between two inverted T7 promoters...’s new plasmid, T444T, contains T7 terminators adjacent to the T7 promoters to prevent the incorporation...it is essential to contain outbreaks when they surface. But detection of the virus costs time and money...

...One of the most pivotal decisions many PhDs face when transitioning from academia to industry is whether...requires deliberate preparation and a willingness to embrace new responsibilities. After my second postdoc,...permission to explore, reflect on your priorities, and embrace these transitions as an integral part of your broader...

...guided by a gRNA is useless without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases...now, it’s time to cast aside those concerns and embrace a new resource to solve the problem: CasPEDIA, ...

...forms tRNA-gRNA and tRNA-gRNA-tRNA, with short 5’ spacer sequences preceding gRNAs, to test for gRNA cleavage...predicted with no addition of nucleotides to the 5’ spacers. The 3’ ends were modified slightly with either...once, and each <200 bp tRNA-gRNA takes up half the space of a standard pol III-gRNA, which will facilitate...

... There is essentially no better place for a scientist to make new relationships than at scientific conferences...rut of hanging out with only your lab mates (let’s face it, you see enough of them).  Before the conference...students and postdocs. There are many inexpensive/free places to order cards. There are a few good reasons to...

...biggest difficulties that plant biotechnologists face is public perception of genetic modification (GM...and we’d love to hear about the obstacles you’ve faced as a plant scientist, and how you were able to overcome...Bioengineering Follow RevBio on Twitter Like RevBio on Facebook Read more Addgene blog posts about synthetic...