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We narrowed to 704 results for: ANG

Showing: 201 - 220 of 704 results

  1. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ... 2018 and last updated Jan 27, 2021 by Jennifer Tsang. We have some crisp new CRISPR material for you!...template, CRISPR can be used to introduce a functional change in the DNA sequence. Base Editors: Modified versions...versions of CRISPR nucleases that make single base changes in the DNA sequence without completely cutting ...other Cas proteins continue to expand the targeting range. CRISPR can be difficult to deliver. Not all cells...

  2. Editor's Choice, October 2016

    Type
    Blog Post
    Published
    Nov. 4, 2016, 2:30 p.m.
    ...had record readership with over 75,000 views. Changing Labor Laws Bring Increased Postdoc Wages Our most...information for anyone considering a postdoc. In “Changing Labor Laws Bring Increased Postdoc Wages,” Future...for some of these institutions to manage these changes quickly and harmlessly. Learn much more about the...Reasons to Use Reddit for Science Communication Changing Labor Laws Bring Increased Postdoc Wages I hope...

  3. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ... causes this dip. 11:11 - 15:37 : How have you changed throughout graduate school? - The importance of...to give to younger graduate students   Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring... studies developmental gene regulation with Dr. Angela DePace in the Department of Systems Biology, and... genetics at the University of California, Los Angeles. She enjoys chromosome biology and reading Ben ...

  4. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    Published
    April 22, 2021, 1:15 p.m.
    ... Zoom’s default backgrounds all year and want a change? Addgene has you covered. Today we’re releasing...Plasmids 101 videos and needed a break so they're hanging out with Aavery in this Zoom video background. ...plasmids as well as transfer plasmids. Download the orange lentivirus background.         We also have ready-to-use...our fluorescent protein background. Download the orange fluorescent protein background.           Did...

  5. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...) are fluorescent proteins that display unique changes in their spectral properties upon exposure to a...fluorescent states to high fluorescence states, they can change from one fluorescent color to another, or their...Activating Light Pre/ Post Color Contrast (Fold Change in Fluorescence) Brightness Excitation/ Emission...PMID:26167640. PubMed Central PMCID:PMC4597790. 14.Wang, Yingxiao, John Y-J. Shyy, and Shu Chien. "Fluorescence...

  6. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...dye emits green, yellow and orange photons while PE emits yellow and orange photons. If FITC and PE were...of the protein. In some cases this can lead to changes or a complete loss of protein function. In addition...determine the relative contribution of yellow and orange from FITC and that from PE. During compensation...10.1002/cpim.40 Picot J, Guerin CL, Le Van Kim C, Boulanger CM (2012) Flow cytometry: retrospective, fundamentals...

  7. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...Patterson GH, Ting LM, Wang Y, Condeelis JS, Verkhusha VV. A photoswitchable orange-to-far-red fluorescent...proteins Newly developed photoconvertible FPs like PsMOrange and mEos add another layer of complexity to imaging...fluorescent protein, PSmOrange. Nat Methods. 2011 Jul 31;8(9):771-7. doi: 10.1038/nmeth.1664. PubMed. Find ...

  8. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang B. Cell. 2013 Dec 19...inter- and intrachromosomal dynamics, and how they change in response to the cell cycle or other stimuli....cells. Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. Proc Natl Acad Sci U S A. 2015 Mar...

  9. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...indicate the amino acid change as well as any functional consequences of that change. For example, "Mutated...number of unique plasmids in a pooled library can range from a few hundred to millions. Pooled libraries...through Addgene. For example, you could enter "Feng Zhang (Addgene plasmid #52961)".   The vector type should...by Tyler Ford in January 2016. It was updated by Angela Holmes and Christina Mork in February 2024.  Additional...

  10. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ...to the distinct overhang patterns generated by the nickases; D10A creates 5’ overhangs and H840A creates...creates 3’ overhangs in a PAM-out design.  Exploring nickases for homology directed repair The potential ... benefit of using nickases for HDR is targeting range: using an individual gRNA with WT Cas9, repair levels...

  11. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ... by integrating a SL-insensitive fluorophore (LSSmOrange) as an internal control and a SL-sensitive fluorophore...enzymatic receptor DAD2. SLs induce a conformational change of the receptor which modulates the fluorescence...fluorescent biosensor has a high sensitivity (50 nM-500nM range) and a high specificity for SLs in vitro, and they...acssynbio.0c00192   The CRISPR corner By Jennifer Tsang New CRISPR plasmids are always being added to the...

  12. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...Isaacman-Beck J, Paik KC, Wienecke CFR, Yang HH, Fisher YE, Wang IE, Ishida IG, Maimon G, Wilson RI, Clandinin...doi.org/10.1101/788679 Lin R, Wang R, Yuan J, Feng Q, Zhou Y, Zeng S, Ren M, Jiang S, Ni H, Zhou C, Gong H,...

  13. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...Using video conferencing tools like Zoom or Google Hangouts is a great way to include researchers from different...person Presenting the results from my lab exchange project at the International Cancer Immunotherapy...other in person. One possibility is to do a lab exchange, where researchers spent time in each other’s ...performed the same way at different sites. I did a lab exchange as part of the collaboration I led. This gave ...

  14. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    Published
    Jan. 15, 2015, 1:50 p.m.
    ...in the CRISPR-Cas9 field – Jennifer Doudna, Feng Zhang, and Keith Joung. All Addgene depositors! Addgene...Addgene was mentioned specifically in Dr. Zhang's introduction. His willingness to share reagents so freely... are taking the "genetic code" to a new level, changing it from a puzzle to decrypt to a tool to be harnessed... tools – both deposited and distributed – have changed so dramatically in the past 4 years. I kept busy...

  15. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...trees and comparing them with the gene expression changes of more than 100,000 cells undergoing iEP conversion...tags often had similar gene expression pattern changes and reprogramming outcomes, suggesting that outcomes... expression and clonal history associated with changes in cell identity. The Morris Lab used CellTagging... processes where cells are undergoing identity changes, such as the differentiation of induced pluripotent...

  16. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...for our first post, I thought it best to define language you’ll see throughout the posts and introduce ...still considered scientific communication if the language is aimed at a peer audience.  Note that lay people....      Jargon is field-specific terminology or language. This can refer both to terms that are only used...number of characteristics. Common ones include age range, familiarity with the subject, shared interests,...

  17. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...work? As illustrated in the Figure below, the “A” overhang on the blue PCR product insert comes from using...TOPO kits provide vectors or cloning arms with overhanging 3´ deoxythymidine (T) residues that are covalently...positive clones. Once you introduce your 3’-end “A” overhang insert, the magic of TOPO cloning happens. Basic...methods to ensure your insert retains the 3' A overhang: Use a mixture of proofreading enzyme and Taq...

  18. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...final plasmid by sanger sequencing. DNA replication by PCR has error rates that range from roughly 1 per...interest. You can use similar processes to add overhangs to your insert of interest for Gibson assembly...chance for mis-priming, so you can use a pretty wide range of annealing temperatures, but you may need to increase...

  19. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...phaffii (formerly known as pichia). What is this new-fangled yeast? It’s actually not new at all, but it’s not... Ploidy Usually diploid, but spontaneously changes Usually haploid Usually haploid Pathology...workhorse. This yeast can also give more protein bang for your media buck - it grows at higher cell densities...their peroxisomes will proliferate upon a media change into methanol and peroxisomal autophagy can be ...

  20. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...the spatial and temporal arrangement of chromosomes and how these arrangements may affect nuclear processes...known for its ability to bind DNA targets without changing them. Thus, it has been widely adapted for a wide... Jennifer Tsang...
Showing: 201 - 220 of 704 results