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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 521 results for: ATO

Showing: 201 - 220 of 521 results

  1. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...applications. Also, purifying GST by affinity chromatography requires proper folding of the tag, which prevents...to be purified by single step metal affinity chromatography. Purification of His fusion proteins from E...

  2. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...used to target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA...itself, simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer ...

  3. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...line is known to be genetically manipulated, its anatomical origin, if cell line has ever been the target...informative. Spoiler alert: HeLa is the most common contaminator line.  For those wanting to access Cellosaurus...

  5. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...of DNA that can move around in a genome. In a laboratory setting, transposons can be used to both introduce...use of viruses is also important for social and regulatory reasons. 1.) The transposon consists of a mirrored...

  7. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...Washington University in St. Louis provides a combinatorial indexing strategy that simultaneously tracks...2019). Key takeaways of CellTag CellTag is a combinatory cell-indexing tool that combines scRNA-seq and...

  8. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...them. CRISPR Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening CombiGEM is a technology that...Albert Cheng and Mark Wanner from the Jackson Laboratory for Genomic Medicine described Casilio, a system...

  9. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...delivery of Cas9 endonuclease, Cas9 nickase, Cas9 activators, Cas9 repressors, select gRNAs, and CRISPR pooled...expression of a gene under the control of the tet operator. AAV AAVs are the workhorses of both chemo- and...

  12. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...following up on these leads as we speak, with collaborators in Paris and New York. We want YOU to join the...impact on virus replication. While we and our collaborators are starting these experiments, there's so much...

  13. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...relevant science."     - Randall Carter, Contracts Administrator Inferior: How Science Got Women Wrong - and ... Tsang, Science Communications and Marketing Coordinator Non-science favorites  The Mistborn series by...

  14. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...37825  AAV1 pAAV-CAG-GFP 59462  AAV2 pAAV-CAG-tdTomato (codon diversified) 114472  AAV5, AAV8 pAAV-...ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid Serotype Name 99120  AAV1 pAAV-ihSyn1...

  16. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...For my undergraduate degree, I majored in human anatomy, but I also took classes in biochemistry, microbiology...the time I was ready to graduate with my BSc in Anatomy, the University of Otago had established the Centre...

  17. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...SF-Venus-iGluSnFR). iGluSnFR3: Improved Glutamate Indicators for Synaptic Imaging However, the first two generations... 30377363 Aggarwal, Abhi, et al. . “Glutamate indicators with improved activation kinetics and localiation...

  18. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...skills include: Science Reporter Scientific Illustrator Research and Development Scientific Advisor..., including: Livestock Breeder Scientific Illustrator Plant Breeder Zoological Worker Conservationist...

  19. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...bodies do for you? R bodies are robust linear actuators that could be used in a variety of applications...applications, purified R bodies could also perform as actuators in microelectromechanical systems (MEMS) devices...

  20. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...how I got my current job as a Communications Coordinator. Wondering how you can develop more transferable...Theresa Liao (@theresaliao) is the Communications Coordinator for the Department of Physics & Astronomy, University...
Showing: 201 - 220 of 521 results