Skip to main content
Addgene
Showing: 201 - 220 of 455 results

  1. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ... research environment” with a strong focus on publishing, but still made it so she could “get exposure...summarized in a recent Nature Medicine paper. In the publication, Bhang and coworkers used the ClonTracer library...

  3. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...Originally published Apr 30, 2020 and last updated Jul 12, 2021. What started out as a small collection...lab instead of a BSL-3 lab. Jesse Bloom’s lab published a protocol and reagents for pseudotyping lentivirus...

  5. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology... E, Bondy-Denomy J, Corn JE, Doudna JA (2017) Disabling Cas9 by an anti-CRISPR DNA mimic. Sci Adv 3:e1701620...

  6. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...substantial relief from both disorders. As of publication, submissions to the UK, EU, and USA federal drug...cardiovascular disease, inflammatory diseases, blindness, and diabetes. Directions and obstacles for future...

  7. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...Cytochemistry (Vol. 46, Issue 8, pp. 977–983). SAGE Publications. https://doi.org/10.1177/002215549804600813 ...Sturtevant (Ed.), PLoS ONE (Vol. 7, Issue 6, p. e38313). Public Library of Science (PLoS). https://doi.org/10.1371...

  8. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...bacteria can also have serious implications for public health. Bacteriocin plasmids: microbial offense...toxic substances like heavy metals or pesticides, enabling their hosts to survive where others can’t. This...

  9. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...the Fragmid system by sequencing 60 individual assemblies created over five months. When they tested those...were perfectly correct. Because they created the assemblies in duplicate, they were able to confirm that ...

  10. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...name and match the name as it appears in its publication or as it is commonly known in your lab. Plasmid...Distribution status for your plasmids: Hold for Publication or Distribute pending QC. The name of the Principal...

  11. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ..., a pre-made MoClo kit, is a prime choice for assembling an array of targeting constructs and delivering... many different MoClo kits have already been established. Keep an eye out for our next blog on choosing...

  12. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...original, though its maturation is even faster. Published just a few days later, Mohan Balasubramanian and...antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing. In a proof-of-concept study, mouse...

  13. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...they are making fairly remarkable progress on establishing a system for the chemical replication of RNA...created a circRNA website so scientists can view public circRNA datasets and download the custom python...

  14. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...recently there has been a counter movement by the public to access basic equipment and to become involved...first hackerspace which now offers members and the public cheap access to personal manufacturing tools – ...

  15. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...This post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates...these people and bios were likely part of the publicity. Good panels do not waste time on long introductions...

  18. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...constant, with fitness effects more pronounced at 12 doublings than at 6. Key takeaways of Mobile-CRISPRi Mobile-CRISPRi...PubMed PMID: 30675034. Peters, Jason M., et al. "Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi...

  19. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...well defined peptides. Munro and Pelham’s first published peptide tagging experiment involved a peptide ...limitations was described by Schüchner, et al., who published a paper showing that the amino acids neighboring...

  20. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...could make a model of Cas9 based on the structure published by Martin Jinek’s group. We had already made an...or trying to explain your science to the general public, visit the Center for BioMolecular Modeling web...
Showing: 201 - 220 of 455 results