We narrowed to 851 results for: CAL

...signals from these reporters. Lack anatomical context/spatial localization: In vivo imaging with fluorescent... be overlaid onto anatomical ultrasound imaging to show where the bacteria localize in relation to internal...ultrasound fingerprint can be detected. Bourdeau et al calls this method acoustic collapse.  Importantly, acoustic...exciting application for ARG’s: in vivo imaging. Specifically, arg1 expressing E. coli aided in ultrasound...intestinal tract and has been used for 100 years to clinically treat enteric infection and inflammatory bowel..., but lux imaging shows only that the bacteria localize to somewhere in the mouse abdomen. arg1 signal...

...covered in detail here. Classical double-strand break repair (DSBR) In the classical DSBR pathway, the 3’...and Homology Directed Repair (HDR). NHEJ is the canonical homology-independent pathway as it involves the... provided the DNA template used for repair is identical to the original DNA sequence at the DSB, or it...plasmid, etc.), and consists of three pathways: classical double-strand break repair (DSBR), synthesis-dependent...purple arrows) or on the non-crossing strand (vertically at the orange arrows). If resolved dissimilarly...applicable in DSB-induced genome engineering, it has biological importance for the repair of breaks that lack...2281-308; (2013). PubMed. A Mouse Geneticist's practical guide to CRISPR applications. Singh P, et al. ...

...2012) scFv Antibody: Principles and Clinical Application. Clinical and Developmental Immunology 2012:1...being smaller than antibodies. scFvs are often genetically fused to other proteins, which has led to their...with 10-24 copies of the short epitope GCN4, also called a scaffold; and 2) a GCN4 binding scFv that’s fused...is easier and cheaper to generate since it’s genetically encoded.   Translational applications of scFvs...antibodies, there are many scFv-based therapies in clinical trials or already in the clinic (Bates and Power... tumour specific antigen. When these molecules, called Bispecific T-cell engagers (BiTE®s), bind CD3 on...David vs. Goliath: The Structure, Function, and Clinical Prospects of Antibody Fragments. Antibodies 8:...

...promoters and Shine-Delgarno sequences used in this so-called bicistronic design (BCD). The 49 vectors in this...: Čelešnik, Helena, et al. "Biosafety of biotechnologically important microalgae: intrinsic suicide switch... Rogers, Jameson K., and George M. Church. "Genetically encoded sensors enable real-time observation ... Ribosome-Controlled Fluorescent Reporter."ACS chemical biology 11.1 (2015): 31-37. pCri system The Gomis-Ruth...latency in matrix metalloproteinases." Journal of Biological Chemistry 290.8 (2015): 4728-4740. You can find...The gene set was carefully chosen to perform statistically relevant benchmarking of cell-free expression...Marx's lab has engineered and validated a new method called FREQ-Seq. This strategy allows scientists to construct...

...University of California, Irvine University of Massachusetts, Worcester  University of Nebraska Medical Center...University and Medical Center  Weizmann Institute of Science Whitehead Institute for Biomedical Research ...time of request MTAs and deposit agreements to basically instantaneous in many cases.  Suggest that they...your organization below? Don’t worry--we check periodically for possible new awardees, and we’re happy to...Hospital  Brandeis University  Broad Institute  California Institute of Technology Cold Spring Harbor Laboratory...University Rockefeller University  Salk Institute for Biological Studies The Scripps Research Institute St. Jude...

..., soon directed himself to finding “immunological and virological solutions to crises like those and ended...Therapeutics, which was acquired by Ultragenyx Pharmaceutical in 2017. As one of the original employees at...learned more about Addgene. “The mission to share critical reagents such as plasmids and viral stocks for...depositor. KSQ uses a CRISPR-Cas9 platform to systematically test the function of all 20,000 genes in disease...Target Discovery at the Novartis Institutes for Biomedical Research. Drug development was something Stegmeier...genetics.” As a postdoc in Stephen Elledge at Harvard Medical School, he developed the first lentiviral microRNA-based...Associate Professor at Mass Eye and Ear and Harvard Medical School (HMS) where he leads a lab of 25 people....

...Python script called SSFinder, but I find its implementation to be too slow for practical use. The R based...causes malaria). I attribute their success to technical expertise, thoughtful single guide RNA (sgRNA)...the piñata builders - the reviewers. After some physical exertion on the part of the party goers, the piñata...the other hand some software tools make use of empirically derived and/or prior knowledge to inform on the... Hsu et al., 2013) used a combination of both empirical and approximate methods. The details of their ...“high mismatch limit”, “approximate design”, “empirical design”, “single-PAM design”, and “multi-PAM design...sites, scores and the statistics that go into calculating them. For this, some online tools such as Cas-OFFinder...

...form of many, many short DNA sequences called reads. Typically they’ll be in a file in FASTQ format, a...DNA is needed for screening experiments, so the scale of the DNA prep is larger. And since the library...all components of the library are represented. Typically, Addgene uses electrocompetent Stbl4 cells when...ensure a good transformation efficiency. For a typical library, Addgene usually does four to eight electroporation...product because the plasmids in the library are identical except for the insert; there’s no need to sequence...). You can also rank the plasmids by abundance, scaled to 1, to create a “cumulative wealth distribution...distribution” curve, or Lorenz curve. By calculating the area under the curve (AUC), one can gain a sense of the...

...quick turn-around time of Sanger sequencing have historically matched the needs of most molecular biology ...rapid advances in technologies that permit large-scale creation and synthesis (“writing”) of longer pieces... a collection of 96 plasmids on a plate) will typically have a wide range of input quantities. This variation...samples. As mentioned above, one of the primary technical benefits of plexWell™ is that normalized libraries... proprietary chemistry). This turns out to be critically important for getting adequate sequencing depth...via plexWell™ libraries is well suited to large-scale de novo assemblies of short constructs like plasmids...computational biologist with a passion for solving critical sequencing problems. He received his PhD in Bioinformatics...

...Talley Lambert, a Research Associate at Harvard Medical School. The need for a community fluorescent protein...one of the most indispensable tools in modern biological research.  Any microscopist will tell you that...maturation, oligomerization), many of which are dramatically affected by environmental conditions (such as... structure was needed.  It is also difficult to scale such a resource when updates depend on a single ...feature filter spectra and collection efficiency calculations.  With ~80 FP spectra (and rapidly growing, ...Talley Lambert is a Research Associate at Harvard Medical School. He manages the Cell Biology Microscopy ...Microscopy Facility, specializing in advanced optical microscopy. Follow him on twitter or github @tlambert03. ...

...lentivirus and have been difficult to manipulate genetically. To solve this issue, Sarah Teichmann and colleagues...triglyceride or cholesterol contents (what is commonly called bad cholesterol). However, size distribution and...including quantification of abundance, size, and localization in vivo. The LipoGlo system represents a new... (bEBPs) at a sequence upstream of the promoter called the upstream activating sequence (UAS). The Wang...Communications, 2019. PubMed PMID: 31451697. Genetically encoded voltage indicators for use in worms Article... Article contributed by Brook Pyhtila Genetically encoded voltage indicators (GEVIs) change fluorescence...real time when neurons or muscle cells transmit electrical information. Such voltage imaging can be performed...

...Arabidopsis (CIBN), which they call the LADL Anchor. Using specifically designed gRNAs, they can then ...Inducible Degradation (AID) uses plant hormones called auxins to conditionally and reversibly degrade ... for AID. By tagging proteins with a 7kD degron called mAID and expressing the essential plant protein...microorganisms based on metagenomic data. MerMAIDs (Metagenomically discovered Marine, Anion-conducting and Intensely...publicly available metagenomic datasets with biophysical studies and the potential of mining datasets ...transcription factors tethered to dCas9, require long time scales with constitutively expressed proteins that are...nature of genomic looping due to it’s briefer time scale and its inducible and reversible nature. Find the...

...scene. But what about the other yeasts – in the biomedical world, are there even other yeasts? In this blog...The most commonly used yeast by biotech and pharmaceutical companies is K. phaffii (formerly known as ...the system. Since then, multiple biotech and pharmaceutical companies (along with some research laboratories... bread. The following table lists some of the biological commonalities and differences between K. phaffii...the expression of recombinant proteins by the pharmaceutical and biotech industries as a protein expression...modification of recombinant protein products, specifically glycosylation. The native glycosylation machinery...human: The important gene co‑expression modules. Biomedical Reports 7.2 (2017): 153-158. DOI: 10.3892/br.2017.941...

...different signals in a certain region or calculate a colocalization factor to assess the spatial relationship...microscope to see where the target protein is (localization) and how much there is (quantification). But...protein targets and you want to know if they co-localize at the same place in the cell? Or you know that...inactivate the fluorophores in the sample, either by chemical treatment or photobleaching or by stripping out...yourself stuck with red-green images generated automatically from the microscope software, you can always...it, the secondary antibody may be binding non-specifically – try optimizing your blocking and washing conditions... your multiplex-labeled sample to see if the localization and intensity patterns are similar between the...

...Castano Corsino, a junior faculty member at Harvard Medical School/Brigham and Women’s Hospital (BWH). Based...like IMP, bring closer than ever (although not physically) the ideas and experience from talented professionals... IMP USA-Europe not only provide the specific technical and logistic support to increase the chances of...professional opportunities that are outside of our typical boundaries, and will arrive to Spain at one point...international mentors. Universities from five different geographical locations in Spain were enrolled: the University...represented a diverse group of individuals with technical and career path needs, and came from the universities...this first program. Although not everyone got a physical award, all the students experienced great improvements...

...began with only a few inventory items offered domestically, but by the end of 2016, we expanded our viral...2014). It is a dual-vector system, meaning it is typically used in conjunction with a second vector that ...lentiGuide-Puro. The alternative single-vector systems typically express Cas9 and a gRNA from the same plasmid....several benefits. First, the low viral titers typically associated with Cas9-expressing lentiviruses will...Tips for using this plasmid Cas9 expression is typically cytotoxic, which makes generation of high titer...particular signaling pathways in specific cell types (typically neurons). While all of these viruses are popular...expressed in cells that express cre recombinase. Typically, this feature is used to attain cell-specific ...

...Table 2: Effect of Localization on Rosella’s fluorescence emission. Localization pH Emission Color ...improves upon them. Methods for studying autophagy Biochemical assays Long-lived protein degradation: This... Pho8Δ60 mutant protein is expressed. Pho8Δ60 localizes to the cytosol in an inactive form unless autophagy...’s slow and only measures bulk autophagy. Morphological assays Electron microscopy: Electron microscopy... tagging it with GFP allows for tracking the localization or accumulation of pre-autophagosomal structures...will emit red fluorescence regardless of its localization in the cell. SEP’s pH sensitivity means Rosella...color fluorescence Rosella emits based on its localization. Table 1: Summary of Rosella’s excitation and...

...hM4Di was fused to mCherry. To investigate the localization of the hM4Di, we used primary antibodies against...allow a high-spatial resolution of the protein localization. Surprisingly our observations at the electron...expression of the transgene of interest and its localization in the membrane. The lesson we learned from ...Electron microscope images showing ultrastructural localization of hM4Di in monkeys. The pre-embedding immungold...arrows point, respectively, to gold particles localized in the cytoplasm or bound to the plasma membrane...However, in some cases the fluorescent signal may be critical for the experiment. Since the fused protein could...Research Center. Using a combination of in vivo and anatomical techniques, her research aims to gain a deeper...

... mechanisms. Most take advantage of chemical groups intrinsically present on antibodies, such as the primary...Chemiluminescence, which is light produced by a chemical reaction, can be detected using specialized imaging...reactions produce a color change, so they are typically used in microscopy-based assays like immunohistochemistry...conjugates (especially if you start to consider clinical applications). But there are a few other classes...somehow be tightly linked to your antibody. This chemical coupling can be achieved through several different... made to antibodies, such as inclusion of non-canonical amino acids or peptide tags. 5) Strong protein-protein... productive lives without thinking about these chemical reactions. After all, there is an abundance of...

...through our lab using barcoded tubes and plates. The critical pieces of information for a given plasmid are ...plasmid sequence. Other information may also be critical to the maintenance and use of certain plasmids...for the process. Always sequence verify Once the physical sample and its associated information are at Addgene... but, in most cases at Addgene, we check what we call the “insert,” (the gene or genetic element cloned...recover around 750 bp of clean sequence from a typical reaction, or about 250 amino acids worth, so make...comparable success rates and sequence quality, which is critical for the efficient handling of large numbers of...glycerol stock to each of 2 96-well microplates (identical copies, see image above). We then select forward...