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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 659 results for: Clu

Showing: 201 - 220 of 659 results

  1. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...proteins. Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin...translation of an array of different reporters including mScarlet-I, luciferase, antibiotic resistance ...can be used for an array of detectable proteins including fluorescent proteins, antibiotic resistance, and...

  2. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...replication control that are not necessarily mutually exclusive. In the first, the replication initiation factor...Asymmetric plasmid loss occurs for several reasons, including one plasmid blocking replication of another and...incompatibility caused by random assortment of pure plasmid clusters. Molecular Microbiology 56:1430–1440 . https:/...

  3. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    Published
    Sept. 13, 2018, 1:55 p.m.
    ...previously not associated with ricin regulation, including almost all genes involved in the production of...non-targeting guides and 6,750 safe-targeting guides were included in the library. Safe-targeting guides were designed...activity, Morgens et al drew some generalizable conclusions about off-target activity, demonstrate safe-targeting...

  4. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ... bioinformatics pipelining In many use-cases, including ours at Addgene, the overall purpose of a data...workflow management system for scientific pipelines, including bioinformatics. We loved the parallels between...language.  The modern architecture of Nextflow included built-in support for containers, such as Docker...

  5. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...the origin and propagation of the line, and can include assays for authentication and contamination) and...International Cell Line Authentication Committee includes a database listing all cross contaminated or misidentified...working for a variety of pharmaceutical companies including GSK, AstraZeneca-MedImmune, and Infors & Applikon...

  6. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...expression strains to accommodate high protein levels including:  ompT: Strains harboring this mutation are deficient...Rhamnose-tunable T7 RNAP expression alleviates inclusion body formation. Expression vector cannot have ...insoluble globs of misfolded protein are known as inclusion bodies, and can be recovered and purified using...

  7. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...The first time I heard about FRET during a journal club, my guitarist brain automatically thought about ...and several other pairs have been used since - including: mCerulean/mVenus, mCerulean/Amber, mCerulean/...Other issues that can affect FRET measurements include: the brightness of a fluorophore pair, donor:acceptor...

  8. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...have created synthetic promoters, which typically include some combination of other promoter elements, and...promoters for eukaryotes and prokaryotes We have included two reference tables below listing some of the... simian vacuolating virus 40 Constitutive  May include an enhancer. PGK1 (human or mouse) General expression...

  9. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...integrins are key to a diverse array of processes, including cell adhesion, migration, and signaling, as well...available through Addgene’s repository. The collection includes 26 unique antibodies that bind discrete subunits...domains. All bind specific integrin alpha subunits, including αL, αM and α5. IPI’s anti-integrin rAbs are unconjugated...

  10. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...qualitative and quantitative measurement of processes including RNA movement by tagging RNA sequences with an ...Kellenberger et al., 2013). Other variants of Spinach include Baby Spinach, a miniaturized version of Spinach...aptamer fluorophores, more are in development, including Carrot and Radish from the lab that developed ...

  11. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...chloramphenicol-resistant strains, we have also included versions with gentamicin-resistance (amplified...broad group of Gram-negative Enterobacteriaceae including in E. coli O157:H7, Shigella flexneri, Enterobacter...where conjugation may be difficult, we have also included versions of pTOX that encode one of two visible...

  12. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...have been used to purify a variety of peptides including antibiotic efflux pumps and ankyrin seven-repeat...constructs. Some applications of plasmids from this kit include the production of a suicide switch in cyanobacteria...PUF protein fusions reported in the literature include RNA detection, RNA cleavage, and translational ...

  13. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...resources needed to accomplish your science, and can include: equipment, reagents, animals, glassware, and even...the institution/funding source. Major equipment includes large centrifuges, sequencers and analyzers, and... as many of these tools as possible, this even includes clocks, timers, and phones.  As for reagents and...

  14. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...AAVs to broadly spread throughout an organism, including difficult-to-target tissues like the central nervous...AAV pseudotyping. It has broad tissue tropism, including liver, muscle, lung, CNS, kidney, retinal, and...serotypes used to target the central nervous system, including neurons, AAV5 is generally the best choice for...

  15. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ... cascades (reviewed in Abreu & Levitz, 2020), including light-sensitive chimeric GPCRS (OptoXRs) (Tichy...experiments!  First, closely read the publication, including the supplemental data. You’re most likely to find...hardware, optogenetics tools, viruses, and more.  Conclusion   Optogenetic tools provide a powerful way to...

  16. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ... variants that are less sensitive to ethanol, including the “middle” ADH1 promoter pADH1m, which maintains...Liqun Luo’s lab developed the Q system from a gene cluster in the fungus Neurospora crassa. The QUAS promoter...3 in a single modular vector for ease of use! Conclusion This post contains only a brief overview of some...

  17. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    .... Other practices that fall into this category include performing experiments with too few replicates ...institute better experimental design practices including talking to statisticians when necessary and encouraging...Hines Minisymposium on Reproducibility Booklet - Including descriptions of many organizations promoting reproducibility...

  18. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...phenotype in order to conclude that the phenotype is due to an on-target effect. Conclusions Selection of gRNAs...modification of interest, it would be unwise to make conclusions on the basis of the activity of a single gRNA...

  19. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...mentioned above. The initial IMP cohort of 78 mentees included: 47 women (60.2%) 20 graduate students (25.6%...directly attributable to participation in the program included improvements in writing fellowships, giving oral...impressions of the IMP experience. Conference highlights included remarks by the Spanish Secretary of Research, ...

  20. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ...reproducibility of experiments and the validity of conclusions. Antibody engineering also benefits antibody-...antibody. (B) Examples of engineered antibodies, including chimerization, conjugation, and antibody fragments...There are different ways to achieve this goal, including error-prone PCR, degenerate primers, gene shuffling...
Showing: 201 - 220 of 659 results