We narrowed to 364 results for: DUR

1. Antibodies 101: Affinity Tags

   Type

   Blog Post

   Published

   Sept. 19, 2023, 1 p.m.

   .... If you decide not to cleave the GST tag after/during purification, be aware that the tag dimerizes in...

2. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

   Type

   Blog Post

   Published

   May 21, 2020, 1:15 p.m.

   ...gene. In cells where the stop cassette is removed during the recombination step, the effector is expressed...

3. Oh, The Places You Can Go: Careers in Science Communication - Product Development

   Type

   Blog Post

   Published

   Oct. 18, 2017, 1 p.m.

   ...provides scientific editing services. “I was working during the day and editing at night. It was not the easiest...

4. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

   Type

   Blog Post

   Published

   Oct. 3, 2017, 12:11 p.m.

   ...editing of abstracts and speaker biographies.” During the week of our interview, Hans was also busy preparing...

5. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

   Type

   Blog Post

   Published

   Aug. 22, 2019, 12:43 p.m.

   ...grow. Auxotrophy and prototrophy come into play during cloning if you’ve designed your experiment using...

6. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

   Type

   Blog Post

   Published

   July 16, 2019, 12:59 p.m.

   ...activation of light sensing proteins. Furthermore, during the first 2 weeks of life, larvae already exhibit...

7. Networking and Inspiring Young Scientists at Freshers Fairs

   Type

   Blog Post

   Published

   Nov. 17, 2015, 3:30 p.m.

   ... a scientist and what benefits did they provide during your education? Let us know in the comments section...

8. Antibodies 101: Epitope Tags

   Type

   Blog Post

   Published

   May 11, 2023, 1:15 p.m.

   ...ideal with Myc, as low pH conditions are required during the purification, which may affect protein function...

9. Antibodies 101: Epitope Availability

   Type

   Blog Post

   Published

   Jan. 11, 2022, 2 p.m.

   ...conditions? It can help to visualize an abstract protein during your sample prep process, imagining how it starts...

10. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type

    Blog Post

    Published

    Sept. 14, 2021, 1:15 p.m.

    ...bacterial selection marker and origin of replication. During bacterial culture, only the plasmid with the antibiotic...

11. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type

    Blog Post

    Published

    Aug. 11, 2020, 1:15 p.m.

    ...This instability can cause partial loss of an ITR during routine plasmid propagation, with complete ITR ...

12. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type

    Blog Post

    Published

    June 2, 2020, 1:15 p.m.

    ...screens require a low multiplicity of infection (MOI) during cell library construction to ensure that the majority...

13. Meet the New Addgene Board Members!

    Type

    Blog Post

    Published

    April 28, 2020, 1:14 p.m.

    ...journey in biochemistry made the foray into genetics during graduate school at MIT. “I read some of the seminal...

14. The Strength of Story Telling

    Type

    Blog Post

    Published

    Dec. 19, 2017, 2 p.m.

    ...talks and interviews. They even tell short stories during weekly lab meetings. When scientists tell good ...

15. 10 Great Guest Posts We're Thankful For

    Type

    Blog Post

    Published

    Nov. 18, 2016, 3 p.m.

    ...ordered biochemical events” such as those that occur during development. Read this post to learn more about...

16. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type

    Blog Post

    Published

    April 25, 2019, 3:01 p.m.

    ...changes favored blue-shifting more than others. During this process, they identified mWasabi, a bright...

17. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type

    Blog Post

    Published

    Jan. 24, 2019, 2:24 p.m.

    ...samples and visualize. For more details on this procedure, find the protocol here.   Tips and troubleshooting...

18. Science Careers: Unruly Interests Feed Many Paths

    Type

    Blog Post

    Published

    Sept. 26, 2017, 12:12 p.m.

    ...regulation of DNA replication and gene transcription during vertebrate embryogenesis. My interests, though,...

19. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type

    Blog Post

    Published

    Aug. 8, 2019, 1:19 p.m.

    ...following morning - as usually deadlines are pressing. During this second read, I pay more attention to details...

20. Summer SciComm Series: Master of SciComm

    Type

    Blog Post

    Published

    July 21, 2022, 1:15 p.m.

    ... scientific research is, after all, one of the enduring goals of science communication.   The Owheo...