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We narrowed to 677 results for: Eng

Showing: 201 - 220 of 677 results

  1. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ... Schematic overview of ChIP. Image courtesy of Shengliu via Wikicommons.   Okay, let’s do a quick ...such as sequencing or qPCR. Yay!  Where’s the challenge in that?  So while this method is certainly long...cell type, amount of protein, and desired fragment length for your output assay. A longer sonication time...the sample type and size.) It can be quite the challenge, and take a few rounds of adjustments, to get ...

  2. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...year (12% in 2018) since 2005. We’re meeting the challenges of DNA distribution to ensure scientists everywhere...happening. The Addgene team will have some fun challenges this year and I’m excited about this, but definitely...documentaries in 3 languages (Japanese, German, and English) and even on 60 Minutes. We are blessed with a ...appearance and functionality are happening and this strengthens our image in the world. Addgene is now as much...

  3. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...but where the kinases are located, their signal strength, and how long their signals last all impact transduction...the signal must be transmitted by intermediate messengers. Optical control of kinase activity can provide...preserved. References 1. Zhou, Xin X., Linlin Z. Fan, Pengpeng Li, Kang Shen, and Michael Z. Lin. "Optical control...

  4. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...itself. In their CRISPRainbow paper, Ma et al. engineered gRNA scaffolds containing two hairpin sequences...permit labeling, but a newly expressed standard length gRNA would “switch” the system and induce genome...Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.” Nat Biotechnol. 34...Addgene. 4. Kiani, Samira, et al. “Cas9 gRNA engineering for genome editing, activation and repression...

  5. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...scientists can also study the microbiome by using engineered genetic tools and reporter microbes. Let’s take...take a look at a few of these methods below. Engineering gut microbes in situ While the ability to monitor...References Leonard, Sean P., et al. "Genetic engineering of bee gut microbiome bacteria with a toolkit...PMC4674677. Ronda, Carlotta, et al. "Metagenomic engineering of the mammalian gut microbiome in situ." Nature...

  6. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ... immense task (it takes, ooh, approximately the length of your average PhD to achieve!) Each field has...there. In every project, time, attention, and/or length restraints mean you will have to pick and choose...cognitive load changed the way I thought about engaging with learners. If something I was communicating...accessible pieces of communications that drive engagement with your target audience. Thinking about cognitive...

  7. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...drawbacks of the indirect method, lengthening an often already lengthy process. (And while I have it on...protein of interest is often one of the most challenging components of any immunoimaging assay. Secondary...has introduced new options for increasing signal strength.  For the time being, though, secondary antibodies...

  8. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    Published
    Dec. 16, 2015, 3:30 p.m.
    ...improvements in genome engineering! References 1. Slaymaker, Ian M., et al. "Rationally engineered Cas9 nucleases...-target specificity of the Cas9 nuclease with engineered variants: eSpCas9, SpCas9-HF1, & HypaCas9. ...Plasmids Browse our CRISPR Guide Pages Find Genome Engineering Resources ...

  9. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ... preliminary results (Dimitri et al). CRISPR engineering of CAR-T cells is particularly promising because... the first-ever systemic CRISPR therapy. They engineered a CRISPR-Cas construct targeting the diseased...technology is rapidly evolving, and many of the challenges with therapies involve patient safety, effectiveness...2013). Dimitri, A., Herbst, F. & Fraietta, J.A. Engineering the next-generation of CAR T-cells with CRISPR-Cas9...

  10. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...tests.  Labs including Jennifer Doudna’s lab and Feng Zheng’s lab had already developed nucleic acid detections...resources References Biering SB,  Sarnik SA, Wang E, Zengel JR, Sathyan V, Nguyenla X, Van Dis E, Catamura ...NV, Ludes-Meyers J, Nguyen AW, Park J, Wang N, Amengor D, Lavinder JJ, Ippolito GC, Maynard JA, Finkelstein...

  11. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...outskirts of Guangzhouon west of beautiful Mao Fengshan Forest Park. As a national science and technology...one take dried seahorse for?), but there were no English speakers around. Opening talks – Genomics discoveries... sensitizing gene cassettes. Thse vectors are engineered to replace the drug resistantance genes in the...cas9 proteins from different species for genome engineering. The final keynote presentation was by Nobel ...

  12. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ... to be prepared to take on the challenges and be proactive and engaged once you are in a leadership role...online and on monthly phone calls. This has its challenges as meeting in person is often much more productive...different types of people in order to harness their strengths and work effectively. Leadership responsibilities...

  13. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...this complex, developing Cas3 tools has been challenging. It can be difficult to ensure comparable expression..., though consistent expression can still be a challenge. A multitasking Cas Most Cas effectors have one...Harnessing type I CRISPR–Cas systems for genome engineering in human cells. Nature Biotechnology, 37(12),...CRISPR-Cas Systems INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons Additional resources...

  14. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...program itself. Picking a mentor comes with many challenges, and the flood of interview requests and visitation...logistical, financial, etc…) now have options to fully engage in the application process. And even if you intend...a travel day to an online call in response to challenges on their end. Though the quick changes in plans...using the virtual world to your advantage can strengthen your application and help you decide if a program...

  15. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...Nowadays a toolbox of naturally occurring and engineered AAV serotypes that further expands the range ...currently used in clinical trials, a variety of engineered ones have shown great promise in preclinical ... AAV safety profile is excellent and further strengthens its position as the vector of choice for in vivo...being done to further improve the technology: engineering of new capsid variants, designing expression ...

  16. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...immunodetection tools is their strong signal strength. This signal strength is due in large part to their larger...requires animal sacrifice and precludes genetic engineering of a tag or fusion with a reporter enzyme. For...labeled with up to 3 dyes, yielding similar signal strength to a standard IgG secondary antibody (Pleiner ...

  17. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    Published
    July 1, 2025, 1:15 p.m.
    ...this case, the absorbance of light at three key wavelengths: 230 nm, 260 nm, and 280 nm. Nucleic acids exhibit...characteristic absorption spectra across this range of wavelengths with maximal absorption at 260 nm (Figure 1A)...the sample also absorb light in this range of wavelengths (Figure 1B-D). These other molecules can alter...A260/230 ratio of a DNA sample, its absorbance strength is low compared to other reagents, making it more...

  18. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ... Biology (Kimball et al., 2020). Benefits and Challenges CUREs inspire students to take ownership of their...experience on their resumes). CUREs are not without challenges. Real research is messy, and it can be difficult... value of this approach and encouraging them to engage in the problem-solving process. His previous experiences...research experiences are a “CURE” that increases engagement by students and teachers. FEMS Microbiology Letters...

  19. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...., Zhang, Y., He, W., Wang, J., Peng, F., Huang, L., Zhao, S., & Deng, W. (2018). Rapid Regeneration and... reduce your lab’s waste footprint. The Zhao and Deng labs developed a simple method for regenerating ... this many uses of the columns, but the Zhao and Deng labs found they could just make their own buffers...

  20. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...may provide a method for doing just that. While challenges remain in the delivery of these agents, CRISPR...potential clinical applications, Ando et al. recently engineered synthetic phages, each based on the well-studied... Lemire, Diana P. Pires, and Timothy K. Lu. “Engineering Modular Viral Scaffolds for Targeted Bacterial...Addgene Blog CRISPR Methods for Bacterial Genome Engineering Treating Muscular Dystrophy with CRISPR Gene ...
Showing: 201 - 220 of 677 results