We narrowed to 285 results for: Fus
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TypeBlog PostPublishedAug. 23, 2022, 1:15 p.m.... for advice on her baby’s health but adamantly refused to get them vaccinated. A classmate told me that...
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The Crispr Babies Saga Shows the Need for Action, Not More Delays
TypeBlog PostPublishedDec. 20, 2018, 1:15 p.m....incorrectly known as “three-parent babies,” where fused genetic material from intended parents is “rehoused... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog PostPublishedNov. 27, 2018, 2 p.m....as wild lettuce. Aside from their use as food, infusions of L. taraxacifolia leaves are widely used to ... -
FPbase: A new community-editable fluorescent protein database
TypeBlog PostPublishedMay 16, 2018, 1 p.m....environmental conditions (such as temperature, pH, fusion protein, etc...). There are many online guides... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog PostPublishedApril 14, 2016, 2:30 p.m.... functional. Attaching the fluorescent protein fusions allowed us to image R bodies inside cells, and ... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog PostPublishedJune 6, 2019, 12:41 p.m....buoyancy. These proteins allow dissolved gases to diffuse in and out but prohibit water from entering their... -
Antibodies 101: ChIP
TypeBlog PostPublishedMarch 10, 2022, 3:25 p.m....molecular and cell biology techniques. What’s all the fuss? Well, ChIP’s complexities come not from the techniques... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog PostPublishedMarch 17, 2020, 1:32 p.m....catalytically impaired Cas protein (dCas or Cas nickase) fused to a DNA-modifying enzyme, in this case a deaminase... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog PostPublishedJune 12, 2018, 11:40 a.m....Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog PostPublishedMay 3, 2018, 1:48 p.m.... al. saw great results with CasRx and AdmCas13d fused to a nuclear localization signal (NLS). In a HEK293... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog PostPublishedJune 1, 2017, 2:30 p.m....with a plasmid containing a fluorescent protein (fusion) there is huge variation in the fluorescence intensities... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog PostPublishedMay 11, 2017, 2:30 p.m....clarity. But, often these methods don’t agree. Confusing indeed! We've developed a new plasmid toolkit,... -
Top Requested Lentivirus and AAV of 2016
TypeBlog PostPublishedJan. 6, 2017, 3:56 p.m....human muscarinic receptor 4 (Gαi-coupled) DREADD fused to mCherry, all under the control of the human synapsin... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....systems with limited packaging capacity. Still confused about the various tRNA expression strategies? ... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog PostPublishedMay 22, 2014, 4:02 p.m....journals and publishers to choose from, it gets very confusing for scientists writing research articles. What... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog PostPublishedOct. 29, 2019, 2:52 p.m....continuous light. ArcLight Q239 - A GEVI based on the fusion of the voltage sensing domain of a Ciona intestinalis... -
Transferable Skills Guide: Public Speaking
TypeBlog PostPublishedApril 20, 2023, 4 p.m....so you end up acting it out in front of a very confused group of visiting Chinese undergrads. You get ... -
The time and cost required to make a plasmid
TypeBlog PostPublishedMarch 23, 2023, 1:15 p.m....get high volumes of plasmid requests can end up refusing such requests in order to preserve time and money... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m.... is a naturally occurring RNase composed of the fusion of several Cas7 and Cas11 domains. Cas7-11 cuts... -
Antibodies 101: Affinity Reagents
TypeBlog PostPublishedApril 14, 2022, 1:15 p.m....fragment variables (scFvs) are ~27kD engineered fusion proteins similar to a Fab fragment but created ...