...that have been in the repository for many years, has now grown into a collection of over 2,400 plasmids... based on 100 structure-guided spike designs and has improved protein yields and stability. This stabilized...virus spreads around the world, the spike protein has had many opportunities to evolve. Several variants... to accelerate research through sharing reagents has never been so important.     References and ...

...off-target effects of gene therapy, Feng Zhang’s lab has developed a system called DIRECTED (Delivery to Intended...on-target delivery with various delivery modalities and has the potential to reduce negative side effects. This... assays like IHC. In fact, Anti-PSD-95 [K28/43R] has been one of the hottest antibodies in Addgene’s collection... collection since we began distribution and has been verified to perform well in standard IHC and western...

...plasmid transformation, though, a pooled library has hundreds to millions of distinct plasmids, all of...they were prior to amplification. And as anyone who has worked with individual plasmids knows, some plasmids...size and backbone.  Verification Once your library has been amplified, you'll need to verify your library...transduction conditions, you’re ready to transduce! Addgene has a transduction protocol you can follow and a troubleshooting...

... is easy to use, functional in most species, and has many applications. That said, SpCas9 is not the only...SpCas9. Cas13a (previously referred to as C2c2), has several unique properties that further expand the...single A, U, or C base pair. In bacteria, once Cas13a has recognized and cleaved its target RNA sequence as...This system, dubbed SHERLOCK (depicted in figure 1) has been used to differentiate strains of Zika virus,...

...world, is not inert. In all seriousness, EGFP/GFP has a real nontrivial propensity to noncovalently dimerize...artifacts in your experiments. Considerable effort has gone into making FPs monomeric, yet many investigators...dimerizing protein of interest fused to a dimerizing FP has potential to form large stable polymers of dimers... monomerized with an A206K mutation [2]. Addgene has many of these constructs and they are designated ...

...joined Addgene's Advisory Board.  Addgene: Your lab has worked extensively with enChIP systems. Can you describe...advantages? Fujii: In the last several years, my lab has been working on development of technologies for biochemical... systems to Addgene in the near future. Addgene: Has your lab been working on any other technologies that...plasmids. The merit of depositing plasmids to Addgene has been spectacular. We've got more than one hundred...

... to monitor cellular perturbations to how CRISPR has made it much easier to generate mouse models.   ...data from the Kim and Joung Labs showing that Cpf1 has lower off-target editing and similar on-target editing...

...proteins (GFP and YFP) and protein tags (c-MYC and HA). We have also created the Monoclonal Antibody Research...community. Thanks to Our Guest Blogger!   David Soll has been the Director of the Developmental Studies Hybridoma...

...science and our plasmid collection (what science has been hot this year and how do you creatively turn...procrastinating choosing their costume?  As our organization has grown, we continue to have an amazing turnout for...

... Broad Institute, John Doench. CRISPR technology has made it easier than ever both to engineer specific...databases annotate the TSS in different ways, and it has been shown that the FANTOM database, which relies...mismatches that can nevertheless still lead to activity has been established, and can be used to identify sites...Perturbation Platform at the Broad Institute and has worked with many Addgenies to help improve the understanding...e141–e141 . https://doi.org/10.1093/nar/gkw583 Rees HA, Liu DR (2018) Base editing: precision chemistry ...

...Adeno-associated virus (AAV) has many features which make it a great viral vector, but its packaging ... Overlapping  Overview: The overlapping strategy has a region of homology, typically ~400-1400 bp long...Drawbacks:  Generally an overlapping split vector has equal or higher transgene expression when compared...split AAV method While the hybrid dual vector system has a slight advantage over the overlapping and trans-splicing...efficiency of transgene expression from split vectors has been inconsistent. A greater understanding of basic...

...Your new antibody has arrived, hooray! Before you jump straight into the exciting new experiments that...good idea to pause and make sure that your antibody has been appropriately validated so that you can have...application. The goal is to show that your antibody has been validated using at least one of these strategies...is binding to something unexpected. This approach has high specificity, but you need to be aware of any...found a cell line in which the gene for your protein has been mutated using CRISPR/Cas9. You check the mutation...

...in previous animal studies suggested that fasting has a positive effect on cancer treatment (Ref 1). As... mutations and clinical responses. This platform has the potential to become an open-source pipeline and...of the plasmids on her poster is pTomo-Ras which has been deposited at Addgene was used to produce the...

...useful research materials and information. Addgene has been successful in helping thousands of sharing scientists...depositors and partners. The Addgene Scientist Team has been developing expertise in answering questions ...from plasmids in the repository. The Outreach Team has been making sure scientists know about the new initiative...

... S. University of Baroda, India  Devarshi Gajjar has 10 years experience in the field of bacterial and...biomedicine and astrobiology. During the last 11 years he has studied various aspects extreme life on earth including...educational resources for scientists globally, Addgene has partnered with various community organizations and...

... capsid? A: It is our understanding that AAV-BR1 has the same genome size restrictions as other AAV. Therefore...but reproducible success remains elusive.  There has also been a lot of effort in immunomodulation or ...AAV re-administration but I do not think the field has settled on ‘best practices’ for model organisms.  ...

...Addgene has recently announced the launch of an open-access recombinant antibody resource called NABOR...Detailed figure legend here.     The Trimmer Lab has continued to work to make the sequences of monoclonal...collection and make them publicly available. The lab has also expanded their recombinant affinity reagent ...

...partnership was fruitful and a first version of the study has just been uploaded to the preprint repository BioRxiv... platform; while they are a minority, their help has been essential to push certain projects. The idea...year on, our biggest lesson running the platform has been the realization that there is a great untapped...

...makes a good mentoring program. If your organization has a mentoring program, consider tapping into it and... have going on locally. Your university probably has an alumni directory. Alumni who enter their information... own peer mentoring circle. This type of program has many advantages and I am seeing a lot of success ...

...I was excited to see that Addgene has recently added a new feature for depositing labs to its website...anytime. The CSV is a rich source of information and has data on the date, material, requesting PI & organization...