... communicate effectively is part of what makes working in a team productive. Take all the opportunities...conferences on interviewing, resume writing, and networking Practice your English if you need to, go to ESL... a content-useful website, write a blog Pursue speaking invitations by inviting others (be on a speaker...“learn the business” Attend events with local networking and trade organizations Another excellent perspective...People Wish They’d Learned Sooner by Peggy Klaus Looking for a mentor or want to start a peer mentoring ...

...offers all of those same advantages to researchers working in plant model systems. “The cloning is all done...trade Lohmann does have one special tip for anyone looking to try this at home in their own lab: be very careful...along at 99 percent efficiency, simply stopped working. They finally traced the problem back to the endonuclease...in the process. “We had several motivations for making GreenGate available through Addgene,” Lohmann said...system, the more building blocks will be available, making it easier for us to do our work.” References 1...

...you are studying the effect of a new tau protein kinase inhibitor on slowing diseases progression in an...group of mice, the experimental group, with the new kinase inhibitor and a second group, the positive control...control group, with an alternative but well-studied kinase known to prevent tau hyperphosphorylation. You ...experimental protocol but does not receive either protein kinase inhibitor. Since the negative control group does...variability. If one were studying the effects of tau kinase inhibitors on mice, for example, it would be better...noise. This can be done either by modifying the blocking conditions or by substituting for a more specific...

...RNase free” and refrain from talking to friends and colleagues while working with these reagents. Other ...they are relatively small and easy to work with, making them the go-to choice for studying mammalian biology...technique is still in need of improvement. Also when working with CRISPR, one must beware that not all gRNAs...blog post. Finally, always remember that you are working with RNAs, which are prone to degradation by the..., Nakauchi H (2018) Intra-embryo Gene Cassette Knockin by CRISPR/Cas9-Mediated Genome Editing with Adeno-Associated...

... Something that I struggle with as I’ve been thinking about my career options is being an overthinker...overthinker. Being an overthinker can make me scared of taking risks, afraid of leaping forward but I have learned...in a life sciences career but life has a way of making unexpected decisions. As someone who was not necessarily...what my Instagram posts would look like. While working remotely was a different experience, It was helpful... to their roles at Addgene. I had a great time speaking with Quintin Marcelino who is Addgene’s Science...

...Mentor – what do I do now? What are the benefits of taking on my own Mentee? Q1. What makes a good science...participants had certainly realized the importance of seeking out mentoring as an important aspect to their ongoing...Mentor is often one that devotes time and energy to thinking about how to be a great Mentor.    Check Out Angela...on the blog “Tenure, She Wrote” is titled, “What Kind of Mentor Do I Want To Be?” In this excellent essay...the northeast, writes a thoughtful treatise on the kind of advisor/mentor she wants to be for her new lab...

...Anterograde transport is typically used for the trafficking of organelles, such as mitochondria, as well ... dynein, while anterograde transport relies on kinesin. Different rates of speed for various forms of ...particles will infect neurons at different ratios, making these neurons fluoresce in different colors. An... The underlying mechanisms of trans-synaptic trafficking are not fully understood and may be a form of...B.T., Maguire, C.A. and Ramirez S.H. (2017). Trafficking of adenoassociated virus vectors across a model...PubMed PMID: 1381034. Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaj, M.I., Isa, K., Mizukami, H., Ozawa... Resources on the Addgene Blog Retrograde AAV: Making the journey from axon to nucleus Rabies and neuronal...

...Matt Takvorian When COVID-19 researchers started working with us to help them share their reagents, we knew...COVID-19 resources are now available to scientists working in a for-profit setting.  In light of the need ...and able to listen to Addgene’s new approach of working with the industry-based scientific community. Conversations...California Institute of Technology             Johns Hopkins University                           National Institute...from the Michael J. Fox Foundation are used in Parkinson’s research.  The extensive resource from the Structural...

...their careers to get advice on choosing a lab, picking your research topic, starting a lab, and more. ...skills that employers love PhD students to have: working to deadlines, writing up clear and attractive reports...reports, being good at presentations and of course, networking and collaborating.” Getting out of the lab also...out that a PhD teaches analytical and critical thinking skills that are applicable to a variety of careers...passionate about. Check out my previous post if you're looking for more information about the benefits of pursuing...

...self-excising cassette (Dickinson et al., 2015) made this step as simple as heat-shocking to induce the expression...2013), or rescue of mutant phenotypes (unc-119) (Dickinson et al., 2013). These approaches allowed effective...knock-ins were occurring 24 hours post-injection, making screening for knock-ins much more efficient. Pure...these improvements from the Mello lab resulted in strikingly high editing efficiencies, obtaining as many ...e193–e193 . https://doi.org/10.1093/nar/gkt805 Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein...

...structural biologist and protein engineer currently working at Sanofi Pharmaceuticals in Cambridge, MA. When...typical middle age weight and “exercise” consisted of taking walks in the mall. I took action, joined a gym,... potential cures. We encourage any researchers working on ACVR1 or any of the pathways that impinge upon...awareness of FOP. Please consider running with us or making a donation via the link below. Please continue ...structural biologist and protein engineer currently working at Sanofi Pharmaceuticals in Cambridge, MA. His...

...about science to everyone – not just to others working in your own field. Alternative careers are now ...necessarily bad news, but it is an argument for taking the time to hone your communication skills. So,...ll know you are on the right track if they start asking you questions. Remember that people like a good...   Consider starting up your own twitter feed or talking about and sharing some science on the one you already...ocean science, they have good advice for anyone looking to make their science matter. If you aren’t ready...

...recombinant affinity reagents. Addgene is looking forward to working with the community to create a fully ...plasmid and viral services. The first step will be working with the community to build a resource of plasmids... well validated collection. I’m thrilled to be working with Addgene to bring this resource to life,” says...10.1038/520295b Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B,...

...lab. A common strategy to determine the binding kinetics of a purified protein involves immobilization ...moiety, which forms dimers, may influence binding kinetics of the target ligand. Another important consideration...-glutathione. Biotin-based immobilization for kinetic analysis Proteolytic removal of the fusion protein...protein could provide a more relevant kinetic analysis, but the trick is to utilize a second immobilization...outlined here provides a basis to determine binding kinetics using a multitude of methods including surface...

...researching, but I decided I needed a new challenge. Looking for sales and marketing jobs Like most choices ... researchers. Many biotechnology companies are looking for PhD or Master’s level scientists who have knowledge...business-like elements. At this point you may be asking yourself, "Why would you switch the focus of your...the employer was not such that he/she would be looking at where I had published, who I had published with...scientific and marketing knowledge. For most PhDs looking at careers outside of academia, sales and marketing...

... accountability that is one major advantage of working with a mentor.The responsibility of arranging meetings...that need work or growth. Here are some ideas for making your meetings productive: Discuss logistics if...reach all the goals you set, but the process of working on them is where growth happens. Mentors bring ... day. If I skip, I get a bunch of text messages asking me, “What happened? Where are you?” My gym posse...Develop coaching skills Encourage your creative thinking Enhance leadership skills Support inclusion, diversity...

... Otito Izu is a PhD Candidate working under the supervision of Akindele Oluwatosin Adeyi at the University...University of Ibadan, Nigeriaunder supervision of Dr. Akindele Oluwatosin Adeyi, University of Ibadan, Nigeria...research related to space medicine. Now he aims on tracking pollution in rivers by employing synthetic biology...be able to support diverse groups of scientists working in a wide range of research fields and geographical...

...Professor at University of Barcelona. The challenge: Making courses on genetic manipulation more hands on While...meeting with some other professors, I went home thinking about how to solve this problem. I didn’t want...your strategy. I realized that similar types of thinking go into developing gene therapy strategies for...noting any gaps in their knowledge. Practically speaking the format I decided on was to have two teams ...skills like team building, collaborative critical thinking, and communication among others. They additionally...

...much of the primary data one might require when making such a decision remains spread across literature...With thousands of scientists routinely using and thinking about FPs, it would be great to have a centralized...building advanced multi-parametric search queries.  Looking for a bright, monomeric FP published in the last...suggestions. If you’re not sure exactly what you’re looking for, the Table of Fluorescent Protein Properties...that set of proteins.  For computer-savvy users looking to incorporate FPbase data in their own projects...

...important part of being a “good scientific citizen” and making sure you do what you can to help your fellow researchers...consistent way so other researchers can use them, to making sure that methods and protocols are available and... can be published. To a scientist, all of this checking and formatting can feel like yet another hurdle...for being transparent in their communication or making their data and reagents readily available. What...confident that everyone’s enthusiasm for actually making it happen would increase dramatically. I’m not ...