We narrowed to 332 results for: NUC

...antimicrobials using efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. (2014). PubMed... Bikard, David, et al. “Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials.” ...

...translocation trap (ITT) system to analyze signal-induced nuclear translocation of proteins in 2004. In addition,...addition, we have recently developed the oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) method to suppress...

...profiling of off-target cleavage by CRISPR-Cas nucleases." Nature biotechnology 33.2 (2015): 187. Pubmed.... 3. Fu, Yanfang, et al. "Improving CRISPR-Cas nuclease specificity using truncated guide RNAs." Nature...

...Previous research has shown that regions such as the nucleus accumbens (NAc), which is linked to the reward ...cytokine interleukin IL-1 beta in the dorsal raphe nucleus (DRN), a region thought to be involved in these...

...been demonstrated in zebrafish using zinc-finger nucleases, TALENs, and more recently CRISPR/Cas9. Another...deactivation method in zebrafish are short, modified oligonucleotides called morpholinos. When injected into zygotes...

...take the nucleus and put it in a fertilized egg to get edited embryos (replacing the nucleus is still ...

... base editors probably due to the presence of nucleosome or other proteins. You can detect base editing...PMC4873371.  2. Nishida, Keiji, et al. "Targeted nucleotide editing using hybrid prokaryotic and vertebrate...

...that MECP2 binds to methylated CpG dinucleotides and CAC trinucleotides during neuronal maturation (Tillotson...

...results in a polymerase error rate of up to 2% per nucleotide position. The “sloppy” PCR reaction is created...degraded by uracil DNA glycosylase (UDG) and Exonuclease III (ExoIII) (step 4). UDG recognizes uracils...

...unfolding and sliding at upstream standby sites." Nucleic acids research42.4 (2013): 2646-2659. PubMed PMID...selection systems for unmodified Enterobacteriaceae." Nucleic acids research 43.13 (2015): e83-e83. PubMed PMID...

...fluorescence. In Brainbow 2.1, GFP is tagged with a nuclear localization sequence, allowing it to localize ...mouse generates cells that randomly express either nuclear GFP, membrane CFP, cytoplasmic YFP, or cytoplasmic...

... of certain expressed proteins endA, endA1 Endonuclease I (nonspecific cleavage of dsDNA ) mutation ...recombination, increases plasmid stability recBCD Exonuclease V activity abolished Increased plasmid stability...

...include a marker like the Hoechst stain for the nuclei. (See Brewer et al, 2022 for a sample protocol.... (magenta), anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail...

...hairpin RNAs (shRNAs), antisense oligonucleotides (ASOs), zinc finger nucleases, and many others (12). The...

...eukaryotic process where approximately 20-30 nucleotides of double-stranded RNA (dsRNA) results in downregulation...functional APT will convert 2-FA to cytotoxic nucleotides, resulting in death. In the presence of 2-FA,...

...antibody to detect various targets, such as proteins, nucleic acids, or glycans. Fluorophores can also be covalently...staining should have high affinity and specificity to nucleic acids, with minimal background staining. Additionally...

... PCR efficiency from a single reaction set-up. Nucleic Acids Res. 31:e122.Thelwell N; Millington S; Solinas...application of Scorpion primers to mutation detection, Nucleic Acids Research, Volume 28, Issue 19, 1 October ...

... drive expression, is it necessary to add a G nucleotide to the start of my target sequence? A3: The human...Utilization During Gene Targeting with Zinc-Finger Nucleases. G3 Genes Genomes Genetics, 3(4), 657–664. https...

...enzyme cloning, TA cloning, or annealing of oligonucleotides. These entry vectors are used in a Golden ... Liu and colleagues, FokI-dCas9 expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding...

...programmed genome editing by a miniature CRISPR-Cas12f nuclease. On the eighth day of CRISPR, Addgene gave to ...