We narrowed to 457 results for: PAC
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TypeBlog PostPublishedNov. 27, 2018, 2 p.m....FACS. In an additional approach, plasmids such as pACBSR-hyg and pFLP-hyg will be used to create gene deletions...biomarkers, and recently, cancer research related to space medicine. Now he aims on tracking pollution in rivers...
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With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog PostPublishedDec. 6, 2016, 3:30 p.m....lentivirus is not always an easy process. The cloning capacity of lentiviral vectors (~8.5 kb of insert) and ...plasmid DNA that you can use along with lentiviral packaging plasmids to create your own lentivirus stocks.... -
Clear and Accessible Writing
TypeBlog PostPublishedFeb. 15, 2024, 2:15 p.m....the use of alt text to describe images in online spaces. Alt text is used by screen readers to provide ...professional development workshop, “Make a Greater Impact Using Clear and Accessible Scientific Writing.”... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog PostPublishedJan. 25, 2024, 2:15 p.m....a gRNA is useless without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases. The resource...the protein would be helpful to know for viral packaging limitations. Maybe you want to read literature... -
Viral Vectors 101: Biosensors
TypeBlog PostPublishedJuly 6, 2023, 1 p.m....with some of our most popular biosensors already packaged and ready to deliver. Ready to give biosensors...AAV as your vehicle, we’ve got you covered with packaging plasmids! References and Resources References... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m....tricky to find: each of the factors can negatively impact both translation and fidelity of translation, leading.... Or, to quote Ryan Mehl, PhD, GCE has “10x the impact of PCR. But it’s 100x more complicated.” And Ryan... -
Viral Vectors 101: Viral Applications
TypeBlog PostPublishedApril 13, 2023, 1:15 p.m.... these amazing tools in your experiments? Let’s unpack the myriad number of emerging viral applications...just one at a time. Many of these libraries are packaged and delivered with viral vectors instead of a ... -
How Addgene Creates Content
TypeBlog PostPublishedNov. 17, 2022, 2:15 p.m....over their favorite science topics. What better space to do that than in the Addgene blog? Fig...expertise of our antibody team to rely on. High-impact content like the antibody guide always gets an ... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog PostPublishedMarch 23, 2017, 2:30 p.m....offices, Addgene would not be able to have such an impact on scientific materials sharing. I would also like...further fulfilling our mission for an even bigger impact. In early 2016 we surveyed hundreds of scientists... -
Communicating Your Science With Help From ComSciCon
TypeBlog PostPublishedJuly 19, 2018, 1:12 p.m....the work you've done. If you want to maximize the impact of your communication, you will also have to explain...communicate with non-scientists, to establish broader impacts for their work in education and policy and to draw... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog PostPublishedNov. 5, 2019, 1:28 p.m....targeted mutagenesis to generate hyper accurate Cas9 (HypaCas9). This nuclease showed decreased or similar off-target...Engineered CRISPR-Cas9 nuclease with expanded targeting space." Science 361.6408 (2018): 1259-1262. PubMed PMID... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog PostPublishedJune 25, 2019, 12:54 p.m.... life cycles bacterial DNA can be accidentally packaged into the newly created phages. Transfer of this...bacteriophage and plasmid properties. The phagemids are packaged into replication-incompetent phage particles with... -
Antibodies 101: Monoclonal Antibodies
TypeBlog PostPublishedJune 29, 2021, 1:15 p.m....of the antibody - the paratopes - may change and impact the specificity and avidity of the antibodies produced... chain or light chain genes within a hybridoma impacts the specificity, avidity, and number of different... -
AAV Q&A with Tim Miles
TypeBlog PostPublishedMay 17, 2022, 1:15 p.m....why hot dogs are sold in packages of 8 yet hot dog buns are sold in packages of 10…) If you missed the... -
Hot Plasmids - February 2022
TypeBlog PostPublishedFeb. 22, 2022, 2:15 p.m.... of performing similarly to Cas9 screens in a compacted library. Overall, Cas12a’s ability to target multiple...significant off-target effects, yet small enough to be packaged in AAV. A dCasMini-mediated adenine base editor... -
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog PostPublishedDec. 15, 2017, 2:07 p.m.... about and develop technologies that will have impacts on many different facets of life. It also seems...neuroscience. AAV tools from Addgene are already having an impact on the field Not to toot our own horn too much,... -
Addgene: The First Twenty Years
TypeBlog PostPublishedDec. 19, 2024, 2:15 p.m....distributed milestone! We were thrilled to see the impact our repository was having on the scientific community...expanding our viral vectors service to include AAV Packaged on Request! “I love Addgene. I think resources... -
CRISPRainbow and Genome Visualization
TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....editing, but it retains its gRNA-directed targeting capacity. By using a gRNA to target fluorescently labeled...that they could evaluate the degree of chromatin compaction even for this small distance! To the authors’... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog PostPublishedMarch 3, 2020, 2:15 p.m....therapy delivery mechanism, but their small packaging capacity of 4-5 kb limits the delivery of larger transgenes... -
Summer SciComm: Preprints
TypeBlog PostPublishedAug. 16, 2022, 1:15 p.m....scientists know the results, your work won’t have any impact! There are lots of ways scientists communicate...efforts, inspire future research, and increase their impact. Open access and transparency Another major benefit...