We narrowed to 456 results for: PAN
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TypeBlog PostPublishedSept. 24, 2019, 12:55 p.m....source (i.e. primer3) or provided by the major companies selling PCR reagents. Nowadays commercial polymerases...can use the chromatogram to spot whether any discrepancies between your sequencing result and the expected...
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Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog PostPublishedFeb. 28, 2019, 1:34 p.m....Lenny Teytelman, CEO of protocols.io, organized a panel to discuss reproducibility issues in research. But... Schwessinger joined this cause and helped by expanding this team, leading monthly calls, and planning... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog PostPublishedFeb. 2, 2018, 3:20 p.m....Susan. After coming to this realization, Susan panicked. She had always loved science, but didn’t know...science communication] needs. I worked for one company, but there were a ton of others that also did work... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog PostPublishedMay 11, 2021, 1:15 p.m....proportion were found on the plasma membrane (top panel in Fig. 1). Of course, this was problematic, because...hM4Di in plasma membranes in both species (bottom panel in Fig. 1). Our results indicate that the tag protein... -
Working with Nuclear Receptors
TypeBlog PostPublishedJuly 15, 2014, 4:03 p.m....which will feature a simplified user interface, expanded numbers of curated molecules, including 400 or...or so coregulators, and a greatly expanded set of links to datasets and reagents in the field. Additional... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog PostPublishedAug. 9, 2022, 1:15 p.m....-mount IHC techniques on rodent brains and is expanding to more complex brain systems such as non-human...monoclonal antibodies; Addgene collaborates with these companies to create and provide general availability of ... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog PostPublishedJan. 5, 2021, 2:15 p.m.... Yongsub Kim’s lab engineered prime editors to expand their PAM flexibility. Find the engineered prime...some of the new viral preps from recent months: Expanded serotypes are now available for Gordon Fishell... -
Rabies and Neuronal Tracing
TypeBlog PostPublishedMay 29, 2018, 1:51 p.m.... neurons integrated into the brain over time (Deshpande et al., 2013). The study found that at early time...Dec; 18(6): 617–623. Pubmed PMID 19349161. 2. Deshpande A, Bergami M, Ghanem A, Conzelmann KK, Lepier ... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog PostPublishedMay 24, 2018, 1:20 p.m....worm amputations. They also created an online companion resource called “cutting class” which provides...encourage you to deposit them back with Addgene and expand the number of genes that other classrooms and researchers... -
CRISPR in the Clinic
TypeBlog PostPublishedNov. 2, 2023, 1:15 p.m.... functional correction of the gene in clonally expanded organoids. This work, and many others, pushed ...for use in the next few months. Several other companies have followed suite, with Beam Therapeutics initiating... -
High Complexity Golden Gate Assembly with NEB’s DAD
TypeBlog PostPublishedAug. 1, 2023, 1:15 p.m....the data-driven approach was needed to massively expand complexity. The three tools developed, outlined...specific overhang sequences, allowing it to be used to expand already-existing sets. Note that this tool relies... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog PostPublishedJune 27, 2017, 1:03 p.m....imaging biomolecules in live cells (Sustarsic and Kapanidis 2015), which may lead to monitoring of these processes...Central PMCID: PMC2396198. 4. Sustarsic, Marko, and Kapanidis, Achillefs N. "Taking the ruler to the jungle:... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog PostPublishedMarch 23, 2021, 1:15 p.m....2015). Recently the Ward lab created a tool-kit to expand upon these original tools that allows for both ... New INTRSECT viral preps available! We are expanding our inventory of ready to use viral preps from... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog PostPublishedJan. 9, 2020, 1:45 p.m....interests while also staying on top of the ever expanding body of biomedical literature! PubMed PubMed ...researchers, journals, and concepts to help you expand your search. Results in your feed are ranked based... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog PostPublishedAug. 30, 2016, 2:30 p.m....international travel, the Zika virus is expected to expand into heavily populated regions of South, Central...extensive infrastructure of molecular and electronic companion technologies that has transformed the assay’s ... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog PostPublishedAug. 11, 2016, 2:23 p.m....conditions for storage, transport and delivery." Japanese journal of cancer research 93.5 (2002): 598-603...PMID: 12050007. 7. Stockschläder, M., et al. "Expansion and fibronectin-enhanced retroviral transduction... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog PostPublishedDec. 1, 2015, 3:30 p.m....development zone so many science and technology companies and start-ups choose to locate there. I made time...developments in this field. Nobel Laureates and the ever expanding importance of RNA The second keynote lecture was... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog PostPublishedNov. 19, 2015, 3:30 p.m....trait to their granddaughters that reduced their lifespan. Because these dramatic conditions were experienced...statistically significantly associated with reduced life-span, but no changes in the DNA sequence could be found... -
Tips to Make the Most of a Scientific Conference
TypeBlog PostPublishedMay 23, 2019, 12:26 p.m....interview and Expand your professional network Volunteer at a professional organization to expand your skill... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog PostPublishedNov. 5, 2024, 8:32 p.m.... can be produced in many different cell types, spanning both prokaryotic and eukaryotic organisms. Typically... from dedicated adenoviral production labs or companies. Production of both AAV and lentivirus requires...