We narrowed to 447 results for: PAN
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TypeBlog PostPublishedMarch 23, 2021, 1:15 p.m....2015). Recently the Ward lab created a tool-kit to expand upon these original tools that allows for both ... New INTRSECT viral preps available! We are expanding our inventory of ready to use viral preps from...
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Hot Plasmids and Viral Preps - January 2021
TypeBlog PostPublishedJan. 5, 2021, 2:15 p.m.... Yongsub Kim’s lab engineered prime editors to expand their PAM flexibility. Find the engineered prime...some of the new viral preps from recent months: Expanded serotypes are now available for Gordon Fishell... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog PostPublishedJan. 9, 2020, 1:45 p.m....interests while also staying on top of the ever expanding body of biomedical literature! PubMed PubMed ...researchers, journals, and concepts to help you expand your search. Results in your feed are ranked based... -
CRISPR in the Clinic
TypeBlog PostPublishedNov. 2, 2023, 1:15 p.m.... functional correction of the gene in clonally expanded organoids. This work, and many others, pushed ...for use in the next few months. Several other companies have followed suite, with Beam Therapeutics initiating... -
High Complexity Golden Gate Assembly with NEB’s DAD
TypeBlog PostPublishedAug. 1, 2023, 1:15 p.m....the data-driven approach was needed to massively expand complexity. The three tools developed, outlined...specific overhang sequences, allowing it to be used to expand already-existing sets. Note that this tool relies... -
Rabies and Neuronal Tracing
TypeBlog PostPublishedMay 29, 2018, 1:51 p.m.... neurons integrated into the brain over time (Deshpande et al., 2013). The study found that at early time...Dec; 18(6): 617–623. Pubmed PMID 19349161. 2. Deshpande A, Bergami M, Ghanem A, Conzelmann KK, Lepier ... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog PostPublishedMay 24, 2018, 1:20 p.m....worm amputations. They also created an online companion resource called “cutting class” which provides...encourage you to deposit them back with Addgene and expand the number of genes that other classrooms and researchers... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog PostPublishedJune 27, 2017, 1:03 p.m....imaging biomolecules in live cells (Sustarsic and Kapanidis 2015), which may lead to monitoring of these processes...Central PMCID: PMC2396198. 4. Sustarsic, Marko, and Kapanidis, Achillefs N. "Taking the ruler to the jungle:... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog PostPublishedAug. 30, 2016, 2:30 p.m....international travel, the Zika virus is expected to expand into heavily populated regions of South, Central...extensive infrastructure of molecular and electronic companion technologies that has transformed the assay’s ... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog PostPublishedAug. 11, 2016, 2:23 p.m....conditions for storage, transport and delivery." Japanese journal of cancer research 93.5 (2002): 598-603...PMID: 12050007. 7. Stockschläder, M., et al. "Expansion and fibronectin-enhanced retroviral transduction... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog PostPublishedDec. 1, 2015, 3:30 p.m....development zone so many science and technology companies and start-ups choose to locate there. I made time...developments in this field. Nobel Laureates and the ever expanding importance of RNA The second keynote lecture was... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog PostPublishedNov. 19, 2015, 3:30 p.m....trait to their granddaughters that reduced their lifespan. Because these dramatic conditions were experienced...statistically significantly associated with reduced life-span, but no changes in the DNA sequence could be found... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m....AAV1! New INTRSECT viral preps available! We are expanding our inventory of ready to use viral preps from...imaging calcium dynamics in fine structures. We have expanded our recombinase-dependent virus selection to now... -
Tips to Make the Most of a Scientific Conference
TypeBlog PostPublishedMay 23, 2019, 12:26 p.m....interview and Expand your professional network Volunteer at a professional organization to expand your skill... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog PostPublishedFeb. 7, 2019, 2:16 p.m.... a Cambrian-like explosion of software tools accompanying the CRISPR revolution. (See omictools for even... a 96-well plate and comes out of it. Biotech companies are known to have tools that do this internally... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog PostPublishedNov. 5, 2024, 8:32 p.m.... can be produced in many different cell types, spanning both prokaryotic and eukaryotic organisms. Typically... from dedicated adenoviral production labs or companies. Production of both AAV and lentivirus requires... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog PostPublishedMay 4, 2017, 2:30 p.m....al.) Monitor gene expression in the developing pancreas by placing FP timer DsRed-E5 under the control...control of Neurog3 (a gene controlling pancreatic differentiation) in mouse embryos (Miyatsuka et al.) Distinguish... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog PostPublishedSept. 6, 2016, 2:30 p.m....as 30% of all cancers, especially those of the pancreas and colon. Despite the prevalence of Ras mutations...assistance from the research community, will soon expand to include several other important pathways. We... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....reaction or can be purchased 5’-modified from a company. However, unlike an RNA guide, it cannot be produced...snipping DNA, he tries random things (101 lessons on Spanish, C++, guitar, photography) and often dreams of ... -
Tips for Using BLAST to Verify Plasmids
TypeBlog PostPublishedMay 29, 2014, 1:29 p.m..... At Addgene, we use blastn to identify any discrepancies in Sanger sequences, such as mismatches, deletions...determine the potential effect of any nucleotide discrepancies. The blastp and blastx programs are optimized...