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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 13 results for: PAN

Showing: 1 - 13 of 13 results

  1. CRISPR Guide

    Type
    Guide
    ...E., Chen, Y., Whitehead, E. H., Guimaraes, C., Panning, B., Ploegh, H. L., Bassik, M. C., Qi, L. S., Kampmann...., Strand, C., Joung, J. K., Kleinstiver, B. P., Pan, X., Huang, A., & Doench, J. G. (2020). Optimization...effects xCas9 3.7 - mutations in multiple domains to expand PAM recognition, increase specificity, and decrease...recognizes NNTA, a PAM sequence often found in plants, expanding the targeting range for plant genome engineering...differentiation. The system is reversible, with a companion CRISPRon to undo repression using a TET1-dCas9...cleave ssDNA non-specifically. Cas12a also has an expanded targeting range to AT-rich regions, as it recognizes.... (2018). Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science , 361 (6408), 1259–1262...

  2. Antibody Guide

    Type
    Guide
    ...you select. Suggested panels for small multiplex experiments 4-color flow panel: BV421, FITC, PE, and ...fluorophore colors in flow cytometry, but the largest panels used currently are in the mid-forties. When designing...and APC 4-color microscopy panel: DAPI for nuclei, AF488, AF555, and AF647 Antibody Selection Selecting ...again. After filtering, narrow down further by company, price, and other factors like availability and...and shipping time. Choose antibodies from companies that are known to produce reliable antibodies. Be aware...antibody can and often is licensed to multiple companies for sale. Before purchasing, directly compare ...

  3. Adenovirus Guide

    Type
    Guide
    ...such as COVID-19, Ebola virus, and Zika virus. Chimpanzee adenoviral (ChAdV) vectors have also been developed...Troubleshooting Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells More Viral Vectors...Gilbert, S. C., Hill, A. V. S., & Lambe, T. (2017). Chimpanzee adenoviral vectors as vaccines for outbreak pathogens...Adenoviral vector vaccine platforms in the SARS-CoV-2 pandemic . NPJ Vaccines, 6 (1), 97. https://doi.org/10.1038...

  4. Lentiviral Vector Guide

    Type
    Guide
    ...lentiviral genome and the cells that survive can be expanded to create stable cell lines. The cell lines will...is used to sort cells expressing GFP and later expanded into a cell line. More information on generating...Hinrichs, C., Highfill, S. L., Somerville, R. P., Panch, S. R., Jin, P., & Stroncek, D. F. (2022). Genome-wide...

  5. Guide to Using Pooled Libraries

    Type
    Guide
    ... services. For an NGS company near you, please consult these links: NGS Company Resources Science Exchange...

  6. Chemogenetics Guide

    Type
    Guide
    ...has been previously used in human populations in Japan, making it an attractive option for translational...Michaelides M (2017). Chemogenetics revealed: DREADD occupancy and activation via converted clozapine. Science...

  7. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...retroviral genome and the cells that survive can be expanded to create stable cell lines. The cell lines will...cells expressing GFP and later, sorted cells are expanded into a cell line. You can find more information...

  8. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Liver AAV7, AAV8, AAV9 Lung AAV4, AAV5, AAV6, AAV9 Pancreas AAV6, AAV8 Photoreceptor cells AAV2, AAV5, AAV8...Duan, D., Yue, Y., & Engelhardt, J. F. (2001). Expanding AAV packaging capacity with trans-splicing or ...

  10. Promoters

    Type
    Guide
    ...than prokaryotic promoters. Eukaryotic promoters span a wide range of DNA sequences. It is not unusual...

  11. Plan Your Experiment

    Type
    Guide
    ... interest (deletions or small insertions) or (B) span the genome-insert boundary (insertions only). The...

  12. Optogenetics Guide

    Type
    Guide
    ...ChR Variants. The optogenetic toolbox has been expanded by scientists through both the identification ...

  13. Molecular Biology Reference

    Type
    Guide
    ... target cells at a high efficiency. Addgene's expanding viral service offers select ready-made AAV and...
Showing: 1 - 13 of 13 results