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We narrowed to 447 results for: PAN

Showing: 1 - 20 of 447 results

  1. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...  Second, expansion introduces distortions. The goal in expansion is for your sample to expand evenly in...equipment needed for EM or SRM? Enter Expansion Microscopy. What is Expansion Microscopy?  ExM provides a straightforward...the protocol, samples can be expanded to ~4-10x linearly! This expansion physically separates molecules...allow for even expansion.  In some cases, labels are added after this stage.  Step 4: Expand! (Typically...are typically easier to expand than mouse brain slices, which are easier to expand than, say, a C. elegans...and after expansion and measure the degree of agreement between them to ensure the expanded images are...lines in pre-expanded samples can sometimes take on a fragmented appearance post-expansion, but that doesn...

  3. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...both Addgene’s founding and our growth into the company we are today. As part of our 20th anniversary celebration...with six distributors (Table 1), in China, Korea, Japan, Brazil, India, and Mexico. Table 2: Addgene distributors... Summit Pharmaceuticals International 2013 Japan Leehyo Bioscience 2015 Korea San Diego...experiments, to enable virtual learning during the pandemic. Feedback like that lets us know that Addgene’...permissions and paperwork. Through updating and expanding our website, we’ve learned about the importance...

  4. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...answers uninteresting, the panel members all say the same thing and one panel member dominates the discussion...what is important. Choose 4 panelists (maybe 5) and no more. Too many panelists will negatively affect timing...will rarely work with a panel if slides are allowed. Don't put your panelists behind a table. It adds ...Put the panel on a stage if the room is very big, but for an intimate event, put the panelists on the ... the panelists introduce themselves. I do a 1-2 minute introduction. If you chose good panelists, they...nonprofit models that differed from the companies represented on the panel. This "spontaneous" participation...more great tips on running panel discussions see Guidelines for Great Panels by Scott Kirsner and How to...

  5. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...We’re very excited to announce that we are expanding our viral vector service to include a Packaged on... on Request option. Through this expansion, scientists will be able to request an AAV composed of an AAV... vector prep.    Excited? So are we! This expansion will give researchers increased flexibility when...when planning their research and expanded choice when it comes to ordering high-quality materials from...overview in this blog post.     When will the expansion happen?  You will be able to request Packaged ... their material page. Interested scientists can expand the banner to select their serotype of choice, ...through our distributor partners in China, Brazil, Japan, Korea, Mexico, and India. Once the service is launched...

  6. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...may be time, then, to look towards genetic code expansion (GCE): techniques in which non-canonical amino...introduced to the bacteria using plasmids. Rapid expansion occurred, with hundreds of new ncAAs and techniques...number of experiments with. The catch The rapid expansion of GCE led to the creation of many new tools and...Tan, Z., & Cornish, V. W. (2021). Genetic Code Expansion: A Brief History and Perspective. Biochemistry...

  7. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...REC3, and PAM interacting domains and allows for expanded PAM recognition as well as increased specificity...synthetic gRNA) are derived from the same species. Expanding the CRISPR toolbox The isolation of novel CRISPR...

  8. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ... many academic research labs closed during the pandemic, it’s hard to get these reagents. However, when...cell lines, and much more have been critical for pandemic research. Image from Reproducibility for Everyone...additional cell line used in this study came from the Japanese Collection of Research Bioresources Cell Bank,...Biomedical Innovation, Health, and Nutrition in Japan. These results  will help researchers choose the... having access to plasmids during the COVID-19 pandemic. Over 7,000 plasmids for COVID-19 research have...strains Reagents developed during the SARS-CoV pandemic more than a decade ago are now being used to study... continued success of repositories, during the pandemic and in the future, depends on support from the...

  9. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...busy in those early years, the founders knew that company culture and enjoying your job were important parts...in between). Eleven years later,  Addgene is a company of fifty instead of three, and we are sending hundreds...Though there still isn't time for goofing off, the company culture here at Addgene has remained paramount....Most Addgenies reply with an enthusiastic, “Our company culture! Addgene is the best place to work, we ...work, we genuinely enjoy each other’s ideas and company. The Addgene summer picnic This past weekend was... of University Park at MIT.  I’m sure at some companies, employees wouldn’t be caught dead coming into... end, it was another successful event! Addgene company culture At Addgene we organically developed the...

  10. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...build your first panel! Designing a Panel In this example, I will describe a basic panel (Table 2, Figure...your "panel". In this blog post, we'll discuss the principles and process of designing a flow panel.  Principles...excitation and emission Before we design your first panel, we will introduce some background on the color ...laser type. Table adapted from: https://emea.bd.com/panel-design/en/flow-cytometry-fluochrome-brightness-spillover...fluorophore. Bleeding Before we start building your first panel, we'll talk about one last principle of color emission...fluorophore-conjugated antibodies together in the same color panel. In this case, make sure to compare the excitation... Hence, they should not be used within the same panel.     Figure 4: Excitation and emission spectra...

  11. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...here and now. Our closure was earlier than most companies. Since the start of this crisis, we’ve been very... in our building that someone from a different company had tested positive for COVID-19 (it turned out...viral vector distribution is recovering from the pandemic-related slowdown. More scientists are sharing ...

  13. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    Published
    March 17, 2020, 1:32 p.m.
    ...represents the major difference between PACE and PANCE: for PANCE the phage progeny is not diluted out but gets...et al., 2016) and since then has been trying to expand their precision editing capabilities. Base editors...with many Cas domains beyond SpCas9. In order to expand the gene-editing applications and targeting scope...PACE) and Phage-Assisted Non-Continuous Evolution (PANCE) selection systems to engineer better and faster...selection, and replications per day.  The PACE and PANCE systems use M13 bacteriophage, which rely on the...and flexible adenine base editor Lower-stringency PANCE, followed by higher stringency PACE, resulted in...

  14. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ... a startup company. However, like many like-minded people, I quickly realize that a company requires more...during graduate school and turn it into a biotech company. Transitioning an idea from academia to a startup...not remove himself from the idea of starting a company around engineered bacteriophages. He decided to...Development & Operations. Starting a biotech company When and how does a startup become "successful...cornerstone of it all. Without a good nucleus, the company won’t go anywhere. The team should initially consist...conversation about its goals and vision for the company; this will help avoid future complications and ...that waste money and time. Most importantly, “the company does not become real until someone takes the risk...

  18. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...gain insight into protein structure and function (Pan et al., 2018). cDNA libraries, which consist of plasmids...Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M, Weissman...517:583–588 . https://doi.org/10.1038/nature14136 Pan J, Meyers RM, Michel BC, Mashtalir N, Sizemore AE...

  19. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...Kv. C) Cultured rat hippocampal neuron expressing pan-membrane ASAP5. Solid blue circle marks the initiation... soma via the Kv2.1 targeting motif as well as a pan-membrane construct without the Kv motif. Altogether...and the soma. The initiation site identified in panel (C) is indicated; red line is exponential fit. Adapted...

  20. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...with Zebrabow Before the generation of Zebrabow (Pan et al., 2011), Brainbow was also used in zebrafish...Nature 450.7166 (2007): 56. PubMed PMID: 17972876. Pan, Y. Albert, et al. "Multicolor Brainbow imaging in...cutaneous melanoma induced by mutant BRaf arises from expansion and dedifferentiation of mature pigmented melanocytes...
Showing: 1 - 20 of 447 results