We narrowed to 289 results for: Pol;
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TypeBlog PostPublishedJan. 31, 2023, 2:15 p.m....amplify your signal - giving you the benefit of a polyclonal, with the reproducibility of a (recombinant) ...
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Finding and Joining Your Dream Lab
TypeBlog PostPublishedApril 8, 2014, 2:50 p.m....or contract, you should be starting to send out polite emails with your CV to people you’d like to work... -
Communicating Your Science With Help From ComSciCon
TypeBlog PostPublishedJuly 19, 2018, 1:12 p.m....broader impacts for their work in education and policy and to draw attention and support to work in their... -
Plasmids 101: Inducible Promoters
TypeBlog PostPublishedJan. 18, 2018, 2:34 p.m....Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog PostPublishedMay 11, 2017, 2:30 p.m....exciting computational algorithms, such as SIFT or PolyPhen to approach this challenge, using attributes of... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog PostPublishedMarch 30, 2017, 2:30 p.m....PMCID: PMC3773023. 7. Vouillot, L., Thélie, A. & Pollet, N. Comparison of T7E1 and surveyor mismatch cleavage... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog PostPublishedMarch 17, 2020, 1:32 p.m....III. Gene III is under the control of a T7 RNA polymerase and cannot be expressed until the base editor... -
AAV Q&A with Tim Miles
TypeBlog PostPublishedMay 17, 2022, 1:15 p.m.... before considering the need for some manner of polyA. In this scenario, you may be best served by a dual... -
Hot Plasmids - February 2022
TypeBlog PostPublishedFeb. 22, 2022, 2:15 p.m....individually (~20,000 lanes/50 mLs) or through two polycistronic expression vectors (3570 lanes/50 mLs) coexpressing... -
Sweating the Small Stuff: Details in the Lab
TypeBlog PostPublishedFeb. 8, 2022, 2:15 p.m....at odd hours, used more secluded workspaces, or politely asked to not be disturbed when they were at the... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog PostPublishedFeb. 1, 2022, 2:15 p.m....Antibodies 101: Monoclonal Antibodies Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies... -
Antibodies 101: Isotypes
TypeBlog PostPublishedOct. 19, 2021, 1:15 p.m....lot of ways to classify antibodies: monoclonal, polyclonal, scFvs, nanobodies, and the list goes on. But... -
Viral Vectors 101: Biosensors
TypeBlog PostPublishedJuly 6, 2023, 1 p.m....2016 Jun 30;60(1):1-8. DOI: 10.1042/EBC20150001 Leopold, A. V., Shcherbakova D. M., Verkhusha, V. V., Fluorescent... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog PostPublishedFeb. 2, 2021, 2:15 p.m....previously mentioned, enlist the help of your network to polish the final draft prior to submission. When in doubt... -
A Deep Dive into BioBuilder
TypeBlog PostPublishedOct. 11, 2017, 1:44 p.m....whose work is focused on education and education policy. Additional Resources on the Addgene Blog Synthetic... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog PostPublishedFeb. 10, 2016, 3:30 p.m..... Using commercially available high-fidelity polymerases such as KOD and Phusion, which are recommended... -
dTAG - You're it!
TypeBlog PostPublishedJune 21, 2018, 2:06 p.m....proximity with the target protein, leading to polyubiquitination of the target protein and proteasomal degradation... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m....spectroscopic methods (based on fluorescence polarization) can be used to detect homodimerization. However... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog PostPublishedSept. 24, 2019, 12:55 p.m....companies selling PCR reagents. Nowadays commercial polymerases are very efficient, but if you are having difficulties... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog PostPublishedNov. 3, 2020, 2:15 p.m.... have a higher conductance, so they can still depolarize neurons at lower MOIs. Figure 2: ChRgers...