We narrowed to 859 results for: STI

...following unanswered questions about CRISPR screen design. Unanswered questions about genome-wide CRISPR...exist to control for these faults. However, it’s still unclear if similar pitfalls exist for CRISPR screens... rates than their non-targeting counterparts, suggesting that safe-targeting guides are more toxic than...closely mirrors that of gene-targeting guides, suggesting that safe-targeting guides better control for...also reproduced several previously observed characteristics that influence gRNA off-target activity: 1)...presents a strategy to control for these effects. By testing thousands of guides for off-target activity, Morgens...

...Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86], target distinct epitopes on the SARS-CoV-2 nucelocapsid protein...fluorescent signal at baseline voltage), the investigators found that administering these AAVs with a dilute...has developed REVeRT, for gene delivery using reconstitution via mRNA trans-splicing. By splitting a large...their mRNAs, the functional gene product is reconstituted within the target cells (Figure 6).   ...Illustration of split fluorophore assay to test reconstitution via mRNA trans-splicing. A cis-splicing vector... The authors used REVeRT to functionally reconstituting coding sequences for a range of applications...introduction of extraneous genetic material to reconstitute the gene of interest, reducing the immunogenicity...

...experiment-based investigation of a hypothesis: proper controls! For every experiment, an investigator needs a ...the effect of the independent variable you are testing. Importantly, they demonstrate the functionality...associated with the independent variable you are testing and not some other unintended factor. Control plasmids...control plasmids would address and answer these questions. Types of control plasmids Part of planning your.... An internal control may be a plasmid that constitutively expresses a reporter protein (e.g., GFP or ...tenet of responsible scientific inquiry and investigation. References: 1. The problem of pseudoreplication...

...AnltWfKuQg pic.twitter.com/OOrwo9feyJ — BTC Institute (@BTCInstitute1) December 23, 2020   Deck the Lab: ...Edition Winners In a tie for first place, the Vestibular Lab decorated not just their lab, but their entire... entire floor, and put together a festive video to showcase it all!           View this post on...TarlingVallim) December 19, 2020 In third place, the BTC Institute decorated with dozens of individual virus snowflakes...cookies! 🤗 🤗 🤗 We hope everyone has a safe and festive Holiday season. 🙌 🥂 🎄 🎉 😷 Remember wear a ...

...genome-editing tool but it could be a fantastic add-on to a diagnostic toolkit, called DETECTR, which has...iceberg as it has been predicted that many others are still unknown. Identifying Cas14 variants In this quest... from the Department of Energy’s Joint Genome Institute for uncharacterized genes that were proximal to...presence of a predicted RuvC nuclease domain, characteristic of CRISPR-Cas enzymes. In contrast to other...middle of the gRNA to be activated. Cas14 in diagnostics By cutting ssDNA but not dsDNA Cas14 is not a...

...are packaged, the physical size of the genomes is still ~4.7kb. But despite this, larger functional transgene...long between part A and B of the transgene to reconstitute the full-length transgene (Chamberlain et al...site donor and acceptor sequences are used to reconstitute the two pieces of the transgene.The AAV encoding...pre-mRNA of the two halves of the transgene will reconstitute the full-length transgene.  Drawbacks:  Trans-splicing...half of the transgene. The full length gene is reconstituted by either the overlapping or the trans-splicing... template’s integration so that the site is reconstituted after integration; and a sequence of stuffer...since there are two ways for a transgene to be reconstituted, there is currently no one ideal split vector...

...cyanobacterium Synechocystis sp. PCC 6803." Biology open (2016): bio-017129. Hsia, Justin, et al. "Modular...you’re an academic researcher at a nonprofit institution, you can order plasmids covering a wide range...how they’ve been used to date . If you have any questions, please write to us in the comments section.  ...Alexandrov lab at the University of Queensland’s Institute for Molecular Bioscience has provided a solution...library of eGFP-tagged human ORF clones to allow testing and comparison of different cell-free expression...The gene set was carefully chosen to perform statistically relevant benchmarking of cell-free expression...

...phosphatase treatment, substituting cytidine with 5-methylcytidine, and/or substituting uridine with pseudouridine...behind at the site of excision, this approach is still not considered safe for therapeutic applications...screening cells for the presence of transgenes is still an important control. Additionally, Sendai virus...The typical drawbacks of using transfection will still exist: transfection efficiency is cell-type dependent...by dilution with each cell division, but it can still take several passages for the minicircle to be completely... that ⅔ of subclones generated from EBNA1 iPSCs still had episome DNA present. DNA-Free Methods RNA...reprogramming proteins for 8 hours a week for 6 weeks, and still required further culturing to generate iPSC colonies...

...Zika Virus As always, if you have any questions or suggestions for us, feel free to comment below. Happy...in DNA. The first, contributed by guest blogger Kristian Laursen from Cornell University, is a bit old ...discussed further in this informative post. Interestingly, this technology may make some uses of Site ...Honorable mentions Of course we’ve had many other fantastic posts throughout the month including a first of... Software Engineer Kris Shamloo wrote a very interesting post about how we develop our in house lab management...

...for students, postdocs, and young Principal Investigators (PIs). We've interviewed Addgene depositors ...Medical School) who took the time to answer our questions.   Advice for success in academia How can we...passion? Even if no one else is excited about it, distinguish yourself with this passion. “Love what you are...laissez-faire; theory or practice; business-like or artistic; tech, anti-tech or pragmatic.” Advice for pursuing..., and George Church for answering our burning questions about how they manage and motivate their labs!...Posts in this Blog Series   Our 3 PIs answer more questions, including: Advice for Choosing a Research Project...

...Massachusetts Institute of Technology, Boston University, Johns Hopkins University, the Salk Institute, and the...USA-Europe not only provide the specific technical and logistic support to increase the chances of success, but...evidence of this, you needn’t look any further than testimonials from the students who participated in the program...common problems that many others have faced yet, still, this challenge did not hinder them from advancing... the Karolinska Institutet, among others). Mentors and mentees held scheduled monthly progress video conferences...universities and high level public and private institutions also showed their support and hope for the IMP...

... write test question banks, case studies, and experiments for labs, in addition to testing those experiments...Labs as part of their curriculum by either taking existing curriculum and customizing it for schools or developing...curriculum development team at eScience Labs. She still gets to write curriculum, but has taken on other...responsibilities.“I’d say maybe 25% of my day is still what I did as an SME, and then 75% of the time I... proposals, a few PhD theses, and occasionally posting something science-related on Facebook. “I think...

...of similar interests and goals. The following questions can serve as a guide when selecting a research...impact on your field? You want to choose a research question that people (especially you!) care about. ...project along the way. Advice from principal investigators Tom Ellis highlights the importance of keeping... apart a piece that can address a topic in a mechanistic way using the technology available.” Choosing... your project will be fresh as well.” Don't underestimate the importance of choosing a solid research ...Posts in this Blog Series Our 3 PIs answer more questions, including: Advice for Choosing a Research Project...

...Leiwe and Takeshi Imai from Kyushu University. Stochastic multicolor labeling is a powerful solution for...microscopy-based neuronal reconstruction. To achieve stochastic multicolor labeling, Brainbow used the Cre-loxP...copies of the transgene cassette are introduced, stochasticity will result in a combinatorial expression of...Brainbow was inherently low. This is because the stochastic and combinatorial expression of fluorescent proteins... such vector-based gene delivery systems, the stochastic expression of XFPs is possible by simply introducing...introducing a mixture of XFP genes. Stochastic multicolor labeling is possible when each of the XFP genes...

...sharing on the site. The entry form has 5 general question types and it takes approximately 15 minutes or... have all of your data prepared. All of these questions relate to factors that you should have already...re running your next AAV experiment, use these questions as a guide for designing your future injection...information so we can follow up with you if we have any questions and so that we can give you recognition for sharing... strain. Experimental design & methods: These questions are focused on injection and detection methods...data. Please note that the data hub is currently still in beta version, so we’re always looking for ways...

...came as an undergrad at Worcester Polytechnic Institute. Though some WPI students chose to go the solo...goal, or at the very least, a clear reason for existing. Here are a few types of teams that exist at Addgene... team A Process Team is a group of people with distinct skills who fulfill a long-term function. When ...biochemist, a geneticist, a microscopist, a biostatistician, and that one golden-handed technician who ...responsibilities are. That’s a subtle but important distinction. If I invite someone to be on a project team,...out the discussion themselves, then one has to question whether that person should be on the team. As ...self-interest ahead of the team’s goals, then that team is destined to do poorly. To some extent, it doesn’t even ...

...upon who your challenger was, you might have to drastically alter your strategy. I realized that similar ...As your students play, you can observe, answer questions, and guide them to productive strategies while...they had 10 minutes to expose their strategy, justifying each single element used to build the construct...concepts required by the course and some were so enthusiastic that they took the cards and the board with ...modifying BUILD YOUR PLASMID You can find rules, suggestions, and more details on how to play the game on ...biomedical education for future generations. All suggestions, tips and critiques to improve the educational...

...don’t get the same result. So, then they end up investing time that they thought would be moving forward... build on it going forward. This is not just a question of being honest and straightforward, it’s an important...do we get where we want to be? The part that is still missing is a clear alignment of incentives. Right... in science needs both a job and funding, so institutions and funders, to a large extent, hold the keys...is clearly room to do more. If we could really instill a culture of rewarding good scientific citizenship...UK and then a postdoc at The Scripps Research Institute in La Jolla, California. She joined Elsevier after...

...study 1: Cell lines Many of the reagents used to investigate SARS-CoV-2 were developed for broad use and are.... Seventeen of these cell lines were used to investigate the susceptibility of cells from different organisms... Bioresources Cell Bank, part of the National Institutes of Biomedical Innovation, Health, and Nutrition...choose the best experimental model for further investigation of this pathogen. The sharing and distribution...The Jackson Laboratory, a nonprofit research institution, has been able to rapidly propagate this strain... rapid pace of SARS-CoV-2 research by removing logistic hurdles to continue making materials available...

...students are confronted with the question ‘what now?’ This is often a hard question to answer if you plan on ...These skills show employers that you can fully investigate and solve a problem or complete a project, overcoming...Manager Patent Lawyer Science Market Research Statistician   Attention to detail Patience, accuracy ...including: Science Reporter Technical Writer Statistician Bioinformatician Science Writer Grant Writer...like for someone who currently in the position. Questions you’ll want to find answers to include: Will ...