We narrowed to 351 results for: SUA

1. What Good Citizenship Can Do for Reproducibility in Science

   Type

   Blog Post

   Published

   Nov. 7, 2019, 2:18 p.m.

   ... struggles with reproducibility at some point. Usually it comes up when a researcher decides on a new ...

2. Trading Spaces - Visiting Scientist Edition

   Type

   Blog Post

   Published

   Aug. 22, 2023, 1 p.m.

   ... site is limited, and we all know that science usually doesn’t follow our timelines! Prepare a research...

3. Networking Follow-up: I Met a Person. Now What?

   Type

   Blog Post

   Published

   Jan. 12, 2021, 2:15 p.m.

   ...different ways. This leads me to my last tip: It’s usually not worth connecting with people you don’t like...

4. How to Lead a Great Meeting

   Type

   Blog Post

   Published

   Feb. 9, 2016, 3:30 p.m.

   ...employees. 15-30 minutes weekly or monthly will usually suffice to keep both parties on the same page. ...

5. Management for Scientists: Delegating is Key

   Type

   Blog Post

   Published

   Feb. 20, 2015, 8:30 p.m.

   ...’t delegate tasks assigned to you and you must usually take on all tasks that involve confidential information...

6. Plasmids 101: Mammalian Vectors

   Type

   Blog Post

   Published

   March 25, 2014, 3:15 p.m.

   ...concentration required for efficient selection. This is usually achieved by performing a "kill curve" (basically...

7. Antibodies 101: ChIP

   Type

   Blog Post

   Published

   March 10, 2022, 3:25 p.m.

   ...selective nature of the cutting. While sonication is usually the preferred method, enzymatic digestion can be...

8. Sweating the Small Stuff: Details in the Lab

   Type

   Blog Post

   Published

   Feb. 8, 2022, 2:15 p.m.

   ...page to a few key reminders on a sticky note   Visual Planning  Plate maps, pre-labeling tubes, and even...

9. GPCRs: How Do They Work and How Do We Study Them?

   Type

   Blog Post

   Published

   Feb. 11, 2021, 2:15 p.m.

   ...plasmids to elucidate the role of 14 of the 16 non-visual heterotrimeric G proteins that can be activated...

10. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type

    Blog Post

    Published

    May 26, 2020, 1:15 p.m.

    ...resistance gene linked to an in-frame H2B-GFP. This visual read out allows for easy screening of different...

11. Developing a cold tolerant E. coli using specialized chaperones

    Type

    Blog Post

    Published

    Jan. 28, 2020, 2:35 p.m.

    ...which grow optimally in moderate temperatures (usually defined between a fairly nippy 20°C to a decidedly...

12. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type

    Blog Post

    Published

    June 1, 2017, 2:30 p.m.

    ...conditions. The properties of fluorescent proteins are usually only examined under a few conditions since it is...

13. Why and how to start studying chromatin (with Addgene's help)

    Type

    Blog Post

    Published

    June 12, 2018, 11:40 a.m.

    ...Protein-DNA Interactions CRISPRainbow and Genome Visualization CRISPR Between the Genes: How to Experiment ...

14. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type

    Blog Post

    Published

    Dec. 6, 2018, 1:34 p.m.

    ...assay paper turning it purple, allowing for easy visual detection of the presence of the norovirus. While...

15. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    Published

    Jan. 6, 2017, 3:56 p.m.

    ...rather than endogenous mCherry fluorescence when visualizing hM4D(Gi)-mCherry in fixed tissue. References...

16. Viral Vectors 101: Inverted Terminal Repeats

    Type

    Blog Post

    Published

    Oct. 19, 2023, 1:15 p.m.

    ...domain of one ITR. These small deletions are not usually an issue as they are thought to be self-repaired...

17. GCE4All: Making Genetic Code Expansion Accessible

    Type

    Blog Post

    Published

    June 15, 2023, 1:15 p.m.

    ... you need a non-canonical amino acid, which is usually added to the media for uptake by the cells. Second...

18. How Addgene Creates Content

    Type

    Blog Post

    Published

    Nov. 17, 2022, 2:15 p.m.

    ...not shown.   However, for our blog posts, we usually make figures in BioRender or use appropriately ...

19. Plasmids 101: Screens vs. Selections

    Type

    Blog Post

    Published

    Nov. 10, 2022, 2:15 p.m.

    ...screening experiment. Thus, a limited panel is usually selected. The method you choose to assess traits...

20. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type

    Blog Post

    Published

    Feb. 10, 2016, 3:30 p.m.

    ...is high enough to negate the need for selection usually required in recombineering. REPLACR-mutagenesis...