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Showing: 201 - 220 of 511 results

  2. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ... lab members as possible. Try to get them in a setting out of the lab (coffee, lunch or beer) and in 1...

  3. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ... LDR has also been extended to multiplexed probe sets that allow the simultaneous interrogation of multiple...SNP sites (15). The related LCR technique uses two sets of probes to achieve exponential amplification. ...

  9. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...beneficial for a low number of cells and in vivo settings. Find the sgRNAiBAR library!  Zhu et al., Genome...remove a stop cassette upstream of an effector/reporter gene. In cells where the stop cassette is removed...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids... a high multiplicity of infection, because this setup allows for experimental replicates for each of the...labeling just a fraction of a genetically-defined subset of cells. By labeling fewer cells in a population...

  11. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...break. The Xiao Wang Lab has recently deposited a set of plasmids that helps identify base edited cell ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...compact Cas13 proteins found in the metagenome datasets from hypersaline samples, were engineered and ...

  16. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ... all the boxes’. Once you’ve assessed your skill set and done your research, it’s time to start sending... a project, overcoming inevitable failures and setbacks. There are many roles that actively require these...

  17. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...majoring in biochemistry in college and play the drum set as their personal instrument. They are the oldest...options from the Addgene catalog. These were a miR30 cassette, Syntaxin3, and osa-miR528. My manager, Katherine...

  18. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ... as to how the shared reagents should be used. Setting clear expectations from the beginning makes the...

  20. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...easily achieved with alternative methods. Ready, set, transduce! Making virus can sound daunting, but ...
Showing: 201 - 220 of 511 results