...plasmids get their name? In 1952, Joshua Lederberg set out to clarify the classification of these cytoplasmic...

... lab members as possible. Try to get them in a setting out of the lab (coffee, lunch or beer) and in 1...

... LDR has also been extended to multiplexed probe sets that allow the simultaneous interrogation of multiple...SNP sites (15). The related LCR technique uses two sets of probes to achieve exponential amplification. ...

... the experimental setup and objectives. Most systems can only excite and read a set number of excitation...this is limited by the filter cubes and filtering settings of the equipment. Systematic evaluation of commercially...

...Preventable errors in the design of cloning strategies set experiments back days or even weeks. Primer design...

... most of these exploratory conversations. If you set yourself a goal of doing at least two informational...

...beneficial for a low number of cells and in vivo settings. Find the sgRNAiBAR library!  Zhu et al., Genome...remove a stop cassette upstream of an effector/reporter gene. In cells where the stop cassette is removed...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids... a high multiplicity of infection, because this setup allows for experimental replicates for each of the...labeling just a fraction of a genetically-defined subset of cells. By labeling fewer cells in a population...

...Single-Stranded Adeno-Associated Viral Genomes in Academic Settings. Human Gene Therapy Methods 30:195–205 . https...vector genomes as containing just the expression cassette that we want, it also includes a small amount ...

... and nonspecific in the second. Fatima therefore set out to design and produce two biosensors that could...

...can be surprisingly more rigorous in industrial settings Tight (sometimes impossible) timelines—others ...

... all the boxes’. Once you’ve assessed your skill set and done your research, it’s time to start sending... a project, overcoming inevitable failures and setbacks. There are many roles that actively require these...

...polymerase using your favorite PCR protocol.  2. Set Up TOPO Cloning Reaction: Mix together the PCR product...topoisomerase site inserted into a beta-galactosidase cassette allowing a researcher to perform blue-white screening...

... no idea where to start. Thankfully, my managers set aside time for me to complete an online social media...

...majoring in biochemistry in college and play the drum set as their personal instrument. They are the oldest...options from the Addgene catalog. These were a miR30 cassette, Syntaxin3, and osa-miR528. My manager, Katherine...

... as to how the shared reagents should be used. Setting clear expectations from the beginning makes the...

...easily achieved with alternative methods. Ready, set, transduce! Making virus can sound daunting, but ...

...break. The Xiao Wang Lab has recently deposited a set of plasmids that helps identify base edited cell ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...compact Cas13 proteins found in the metagenome datasets from hypersaline samples, were engineered and ...

...percentage of the recombined vector backbone.   Ready, set, grow! Our 5 Tips for Troubleshooting Viral Transductions...

...establish that schedule. There’s also tips for setting writing goals, tracking progress, and starting ...

...long email. If you have more to say, email again or set up a meeting. If you really got along well, suggest...