Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 608 results for: Sis;

Showing: 201 - 220 of 608 results

  1. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...space where BosLab exists today. Back then, it consisted of “wooden slab benches” set on top of filing ...companies and BosLab has grown into an organization consisting of over twenty members and volunteers. Transforming...BosLab. Once he optimizes plant cell culture and transgenesis he wants to share his knowledge with the community...

  2. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...of other analysis scripts available, some associated with specific libraries, but any analysis script should...hundreds of thousands if not millions of reads, this analysis must be done in an automated manner. Addgene commonly...

  3. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ... way into high school classrooms, like gel electrophoresis to separate DNA or transforming cells with ...practice with practical lab skills like developing a hypothesis, precisions pipetting, and good record keeping...in functional genomics research Learn how the Arabidopsis research center brings plant science to life ...

  4. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...about Synthetic Biology Find Arabidopsis educational resources at the Arabidopsis Biological Resource Center...Promoters, Inserts, Tags, Origins of Replication, Resistance gene and Selection Markers) and one “Transfer...

  5. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...required for replication, packaging, and vector persistence. They work in conjunction with the viral rep ... they flank the gene of interest. A single ITR consists of palindromic arms (A-A’, B-B’, and C-C’) that... also helpful in quantifying ITR mutants since analysis of individual reads allows for identification ...

  6. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...as LEDs can be engineered to deliver precise, consistent, and controllable pulses of light. Complicated... heading of two-component systems (TCSs). TCSs consist of a histidine kinase that phosphorylates a response...Leakiness was also an issue; some promoter activity persisted in the inactive state. The dynamic range, defined...

  7. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...since become a gold standard in gene expression analysis and a luciferase gene (one of many available to...photon emission is measured resulting from the catalysis of a chemical reaction requiring luciferin, ATP...light-producing reaction. Many luciferase assays require cell lysis as the most efficient means to disrupt the cell ...

  8. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...plasmids articles. These brief articles provide a synopsis of a plasmid or group of plasmids' functions and... used a combination of deliberate and random mutagenesis to isolate the best RFP. They targeted known ...-induced mutations in ATP1A1 can render cells resistant to the small molecule ouabain - and editing at...carefully designed to allow for easy removal of the resistance cassette via Cre recombination. Each knockout...

  9. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...monoclonal Cas9 background because you’ll have more consistent levels of Cas9 expression between experiments...pAAV-hSyn-DIO-hM4D(Gi)-mCherry Our current AAV inventory consists almost entirely of viruses expressing DREADDs ... References 1. Aschauer DF, Kreuz S, Rumpel S. Analysis of transduction efficiency, tropism and axonal...

  10. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...upsteam sample collection process or the downstream analysis process. And ChIP itself begins at...  Crosslinking...1000 bp. This is to ensure that your downstream analysis focuses on the DNA your protein is actively interacting...complexes. Your DNA is now ready for downstream analysis via any methods suitable for isolated and purified...

  11. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...Soon you’ll figure out what prompts you need to consistently and accurately run a protocol.   Figure...hasn't. Developing simple habits like these can consistently reduce errors as you work, and the possibilities...maybe especially) suggestions you are initially resistant to. Just like any other experimental approach,...

  12. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...molecular switches in signal transduction pathways and consist of a trimer of three subunits: alpha (Gα), beta...ligand-bound GPCRs of interest. The TRUPATH plasmid kit consists of 20 plasmids encoding 14 alpha, 2 beta, and ..., GPCRs were first mutated via site-directed mutagenesis to bind non natural ligands. These non natural...

  13. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...way to narrow down candidates for more specific analysis, like PCR or restriction digest. Make sure you...colonies that not only have your plasmid (antibiotic resistance), but also confirm those plasmids have your insert...plasmid-containing cells without using antibiotic resistance. These rely on cell lines susceptible to or dependent...

  14. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...Cell and Regenerative Biology is to understand the basis for cardiovascular and metabolic human diseases.... activator-like effector nuclease (TALEN) kit consisting of 834 plasmids. The researchers showed they ... or using a homologous template provided by the sister chromosome. As cells take those two ends and put...

  15. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...this problem, gRNAs can be chemically modified to resist degradation while still effectively serving their...using commercially available kits or ordered as synthesis modifications when purchasing your gRNA. Pro tip... mismatch discrimination and is more nuclease-resistant, resulting in less off-target events. While both...

  16. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...bumble bee and honey bee microbiome. The toolkit consists of modular parts that are assembled with Golden...plasmids and suitability of various antibiotic resistance markers, promoters, and inducible gene expression...into how the microbiome responds to infection, dysbiosis, and antibiotic treatment. To facilitate these...

  17. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...in vivo, they have higher reproducibility and consistency in the final product. Because the constant (non-epitope... as ankyrin repeat proteins. DARPins typically consist of 3-4 repeats, have high specificity and binding...Targeting HER2/neu and CD16 Exhibit Improved Tumor Lysis When Placed in a Divalent Tumor Antigen Binding ...

  18. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...your barcoded gRNA library sequences via oligo synthesis using the format indicated below and pool-clone... Wong et al 2015. Alan Wong is currently an Assistant Professor at the School of Biomedical Sciences...Timothy K. Lu. "Enhanced killing of antibiotic-resistant bacteria enabled by massively parallel combinatorial...

  19. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ..., or other. Bacterial Resistance Enter bacterial antibiotic resistance encoded in the plasmid that...Cloning Method Choose from: Gateway cloning, gene synthesis, Gibson cloning, ligation-independent cloning,...
Showing: 201 - 220 of 608 results