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...prevent alternative start site initiation, the lab removed the native methionine start site. This first ...

...increasingly available. But think of this as a jumpstart or a refresher for your skillset rather than a...organizations that practice science communication or start your own! When you do, let us know!  To get in touch...

...in statistics and programming, you’d be wise to start working on these skills. Neuroscientists use lots...always suggest a plasmid to us, request that we start making the AAV, or help us produce new educational...

...allow researchers to trace the progeny of a single starting cell. Additionally, multiplexing of guides allows...different types of research questions. Are you ready to start EvolvR-ing your targeted mutagenesis experiments...

...manager,” you’re not alone. A few years ago when I started my career transition from bench science to software...solutions with the users, gather more feedback, and start the cycle over again to refine the solution, or ...

... staff receives – in fact, the blog originally started as a way to easily store and share in-depth answers...someone new to antibodies might need to successfully start using them at the bench, along with questions we...

...considerable attention on transcription factors, starting with an established method called ChIP-seq. ChIP-seq...available to anyone for a modest cost without having to start all over or write to us to get them. Even though...

...system and contains 10 sgRNAs for each transcription start site in 15,977 human genes. The transcriptional ...library contains 10 sgRNAs for each transcription start site in those 15,977 human genes. Weissman and his...

...formal mentoring program can be a great way to get started with mentoring. Read this Catalyst Report “Making... sound like a big responsibility or commitment. Start by asking them to have coffee. This is much less...

...scientists. She, Kenneth Fan, and Benjie Chen decided to start an open science nonprofit that would facilitate ...Excellence Award for our electronic MTA system. We also started our blog and YouTube channel in 2013, which continue...

...possible antibody conjugates (especially if you start to consider clinical applications). But there are...make the process quick and easy. Before getting started, be sure to check that your antibody’s buffer is...

...problem solving process on your own, but you can start by paying attention to how your mentors reach solutions...to remember that you have these skills when you start applying and interviewing for new positions. Highlighting...

...also created a miniature yet active Cas system starting with the same Un1Cas12f1 (Cas14). They noted that...and introduce epitope tags and RNA transcription start sites. The lab further demonstrated that PRIME-del...

...protein. Whereas production of monoclonal antibodies starts with selecting just one of these B cell clones ...immune response. Once the initial antibody response starts to wane about a month later, the animal is given...

...some require going back two steps, some require starting over entirely, and our software needs to handle... tricky procedure failed and one of your techs started over with a new tube. In the first example we’...

...each ligation reaction uses a pool of gRNAs as starting material, the ligation products are a diverse ... al., 2014) and microRNAs (Wong et al., 2015). Starting your CombiGEM-CRISPR experiments The first thing...

...to relevant primary research articles. Then just start reading and following citations through the literature...research area. Set a goal for paper-reading, even starting out at 1 paper/week will be useful. Check out ...

...transcriptional units, and multigene constructs. Let’s start by examining individual parts and transcriptional...MoClo Interested in using MoClo? You don’t have to start from scratch! As you can imagine, there is an array...

...yeast), S. pombe (fission yeast), A. thaliana (mustard weed), O. cuniculus (rabbit), synthetic, or other...Like to Find at Addgene Resources on Addgene.org Start Your Deposit Deposit FAQ Which Plasmids Should I...

...’s dCas9… with no modifications). Ready to get started?   Figure 1: Break free from the ball ..., transcriptionally active dioxygenase  When I started to work on building a CRISPR/dCas9-based tool for...