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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 542 results for: TIL

Showing: 201 - 220 of 542 results

  1. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ...include embryonic lethality, a short lifespan, or infertility and can arise due to copy number, expression ...would get both mutations onto the same chromosome. Until crossing over occurs between the two loci, F2 and...

  2. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...inversion, translocation or cassette exchange. By utilizing recombinases to manipulate genomes, scientists...demonstrating low sensitivity to ambient light, and versatile as it can be activated by both low and high-intensity...

  3. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...interaction - one that gives our customers the most utility in the least amount of time. Though I now spend...customer support scientists wear many hats at this still relatively small nonprofit. If you do go longer ...

  4. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...move in the right direction, the increase notably still does not meet the suggested postdoc salary ($50,...advocating for change: from developing a strategy to distilling a message for the appropriate audience and communicating...

  5. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...Figure adapted from Kleinstiver et al., 2016. Until recently, two major elements of Cpf1 characterization...off-target editing in a cell culture model. What we still don't know about Cpf1 In their original paper characterizing...

  6. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...paternal grandmothers who experienced famine while still in their mother's womb passed on a trait to their...time.  This challenge was difficult to overcome until now. Alice Ting’s lab has developed a new, plasmid-based...

  7. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ... thousands of genes with minimal effort. The versatility of Cas effectors allows for a wide variety of...AAV and lentivirus have low immunogenicity, AAV still beats lentivirus. In mice, AAV is almost entirely...

  8. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...wealth of potential, and while many applications are still yet to be demonstrated (or invented), its behavior...results early and often, including works-in-progress still in need of further study and development like MagLOV...

  9. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...Prime editing is a versatile genome editing technology that allows precise modifications of DNA (replacements... (2023). Prime editing for precise and highly versatile genome manipulation. Nature Reviews Genetics, ...

  10. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...and monoclonal antibodies. However, it was (and still is) infeasible to generate highly specific antibodies...EQKLISEEDL) - larger than the substance P peptide, but still quite small. However, unlike the substance P tag...

  11. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...predominantly fluoresce one color but slowly mature until they predominantly fluoresce a second color. This...multiple systems Terskikh et al. showed that their versatile timer could be used in vitro, in mammalian cells...

  12. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...Traditional Cas9 regulators In addition to its utility in making targeted modifications to DNA, Cas9 can.... When complexed with a truncated gRNA, Cas9 is still able to bind DNA but is no longer competent to cut...

  13. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...(Figure 4). When recombination occurs, the gene still functions normally because one of its exons has ...are located in intronic regions, this gene will still be expressed. You'd first screen for this specific...

  14. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ... related to the actual data collection task but still be beneficial to both.  Coming up with creative ...with the company so they know I’m excited while still being able to take my vacation? I want to learn ...

  17. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...impressive, full-color images of proteins. But they are still flat. To truly appreciate the 3D structure of these... will understand the power of a physical model. Until then, you can only wonder what I am talking about...

  18. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...Restriction digest screening for ITR deletion is still necessary. To save time and money, I like to miniprep...on the Addgene blog Find out what makes AAV a versatile tool for gene expression in mammals Use the PEI...

  19. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...expression of the gene it follows. For increased versatility, it would be advantageous to adapt PITCh to insert...-independent knock-ins, but this possibility is still being explored. More broadly, Sakuma et al.’s use...

  20. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ... lines with compound autosomes are completely infertile when mated to wild fruit flies6. It's also worth...develop formal guidelines to answer this question. Until then, using multiple confinement strategies is strongly...
Showing: 201 - 220 of 542 results