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Showing: 201 - 220 of 532 results

  1. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...Figure adapted from Kleinstiver et al., 2016. Until recently, two major elements of Cpf1 characterization...off-target editing in a cell culture model. What we still don't know about Cpf1 In their original paper characterizing...

  2. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...paternal grandmothers who experienced famine while still in their mother's womb passed on a trait to their...time.  This challenge was difficult to overcome until now. Alice Ting’s lab has developed a new, plasmid-based...

  4. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...Restriction digest screening for ITR deletion is still necessary. To save time and money, I like to miniprep...on the Addgene blog Find out what makes AAV a versatile tool for gene expression in mammals Use the PEI...

  5. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ... related to the actual data collection task but still be beneficial to both.  Coming up with creative ...with the company so they know I’m excited while still being able to take my vacation? I want to learn ...

  7. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...and monoclonal antibodies. However, it was (and still is) infeasible to generate highly specific antibodies...EQKLISEEDL) - larger than the substance P peptide, but still quite small. However, unlike the substance P tag...

  8. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...predominantly fluoresce one color but slowly mature until they predominantly fluoresce a second color. This...multiple systems Terskikh et al. showed that their versatile timer could be used in vitro, in mammalian cells...

  9. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...Traditional Cas9 regulators In addition to its utility in making targeted modifications to DNA, Cas9 can.... When complexed with a truncated gRNA, Cas9 is still able to bind DNA but is no longer competent to cut...

  10. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ... thousands of genes with minimal effort. The versatility of Cas effectors allows for a wide variety of...AAV and lentivirus have low immunogenicity, AAV still beats lentivirus. In mice, AAV is almost entirely...

  11. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...wealth of potential, and while many applications are still yet to be demonstrated (or invented), its behavior...results early and often, including works-in-progress still in need of further study and development like MagLOV...

  12. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...Prime editing is a versatile genome editing technology that allows precise modifications of DNA (replacements... (2023). Prime editing for precise and highly versatile genome manipulation. Nature Reviews Genetics, ...

  13. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...impressive, full-color images of proteins. But they are still flat. To truly appreciate the 3D structure of these... will understand the power of a physical model. Until then, you can only wonder what I am talking about...

  14. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...(Figure 4). When recombination occurs, the gene still functions normally because one of its exons has ...are located in intronic regions, this gene will still be expressed. You'd first screen for this specific...

  15. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...expression of the gene it follows. For increased versatility, it would be advantageous to adapt PITCh to insert...-independent knock-ins, but this possibility is still being explored. More broadly, Sakuma et al.’s use...

  16. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ... lines with compound autosomes are completely infertile when mated to wild fruit flies6. It's also worth...develop formal guidelines to answer this question. Until then, using multiple confinement strategies is strongly...

  18. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...period of time. Though climate change certainly still is and will continue to be a “critical consequence...current period of rapid flux, the chapter might still feel slightly dated by next week.   A stepping stone...

  19. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...regeneration, trace amounts of DNA (3 femtograms/ul) could still be eluted from the column, as detected by qPCR, ...break-up with your DNA purification kit, you can still reduce the number of spin columns you need to complete...

  20. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ... absence of genomic alterations. These studies utilize a catalytically inactive form of Cas9 known as ...elements (CAPTURE) (Liu et al., 2017). This system utilizes dCas9 fused to a biotinylation tag expressed on...
Showing: 201 - 220 of 532 results