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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 546 results for: TIL;

Showing: 201 - 220 of 546 results

  1. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...in neuroscience, this architecture is frequently still an open question. The connections between neurons...new neurons came from nearby neurons. It wasn’t until later time points that longer-range connections ...

  3. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ... that is useful, but not 100% complete, we will still make this data available as a partial sequence with...Harten DeMaio K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L, Mueller I, Mitsopoulos A, ...

  4. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ... to modify chromatin. A fluorescent protein can still be included for visualization purposes, but the ...gRNA complex binds the target DNA without cleavage until it's activated by light. Once activated, Cas9 cleavage...

  5. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...interaction - one that gives our customers the most utility in the least amount of time. Though I now spend...customer support scientists wear many hats at this still relatively small nonprofit. If you do go longer ...

  6. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...move in the right direction, the increase notably still does not meet the suggested postdoc salary ($50,...advocating for change: from developing a strategy to distilling a message for the appropriate audience and communicating...

  7. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...Figure adapted from Kleinstiver et al., 2016. Until recently, two major elements of Cpf1 characterization...off-target editing in a cell culture model. What we still don't know about Cpf1 In their original paper characterizing...

  8. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...paternal grandmothers who experienced famine while still in their mother's womb passed on a trait to their...time.  This challenge was difficult to overcome until now. Alice Ting’s lab has developed a new, plasmid-based...

  9. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ... thousands of genes with minimal effort. The versatility of Cas effectors allows for a wide variety of...AAV and lentivirus have low immunogenicity, AAV still beats lentivirus. In mice, AAV is almost entirely...

  10. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...wealth of potential, and while many applications are still yet to be demonstrated (or invented), its behavior...results early and often, including works-in-progress still in need of further study and development like MagLOV...

  11. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ... related to the actual data collection task but still be beneficial to both.  Coming up with creative ...with the company so they know I’m excited while still being able to take my vacation? I want to learn ...

  13. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...predominantly fluoresce one color but slowly mature until they predominantly fluoresce a second color. This...multiple systems Terskikh et al. showed that their versatile timer could be used in vitro, in mammalian cells...

  14. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...Traditional Cas9 regulators In addition to its utility in making targeted modifications to DNA, Cas9 can.... When complexed with a truncated gRNA, Cas9 is still able to bind DNA but is no longer competent to cut...

  15. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...(Figure 4). When recombination occurs, the gene still functions normally because one of its exons has ...are located in intronic regions, this gene will still be expressed. You'd first screen for this specific...

  17. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...Restriction digest screening for ITR deletion is still necessary. To save time and money, I like to miniprep...on the Addgene blog Find out what makes AAV a versatile tool for gene expression in mammals Use the PEI...

  18. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...impressive, full-color images of proteins. But they are still flat. To truly appreciate the 3D structure of these... will understand the power of a physical model. Until then, you can only wonder what I am talking about...

  19. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...Prime editing is a versatile genome editing technology that allows precise modifications of DNA (replacements... (2023). Prime editing for precise and highly versatile genome manipulation. Nature Reviews Genetics, ...

  20. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...and monoclonal antibodies. However, it was (and still is) infeasible to generate highly specific antibodies...EQKLISEEDL) - larger than the substance P peptide, but still quite small. However, unlike the substance P tag...
Showing: 201 - 220 of 546 results