We narrowed to 214 results for: TIR

1. RNA Interference in Plant Biology: New Tools for an Old Favorite

   Type

   Blog Post

   Published

   Oct. 27, 2020, 1:15 p.m.

   ...trivializes downstream characterization, as the entire culture can be harvested instead of microscopic...

2. A Guide to Getting Started in Undergrad Research

   Type

   Blog Post

   Published

   June 8, 2021, 1:15 p.m.

   ...industry, so feel free to explore! Addgene has an entire page dedicated to different career options from...

3. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

   Type

   Blog Post

   Published

   March 7, 2017, 3:30 p.m.

   ...gene editing process. See below for details. The entire process from start to finish took about 1 month...

4. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

   Type

   Blog Post

   Published

   Dec. 15, 2016, 3:57 p.m.

   ...2) if the dsDNA was short enough, a ssDNA whose entire complementary strand was degraded. Beta: Beta protects...

5. Transferable Skills Guide: Teamwork

   Type

   Blog Post

   Published

   Nov. 28, 2017, 2 p.m.

   ...more things necessary to make a successful team. Entire books have been written about this! But this is...

6. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

   Type

   Blog Post

   Published

   June 28, 2018, 3:01 p.m.

   ...Shuttle device; 5 days after the transfection, the entire plate was scanned for V5 tagging using an Operetta...

7. A Needle in a Base-Stack: Cas9 Structural Biology

   Type

   Blog Post

   Published

   June 4, 2024, 1:15 p.m.

   ...the recognition domains—including the CTD and the entire REC lobe—do not structurally resemble any other...

8. How to Deposit Your Plasmids with Addgene

   Type

   Blog Post

   Published

   Feb. 27, 2024, 2:15 p.m.

   ...assembled or theoretical — it does not have to be entirely verified by sequencing. Upload any support files...

9. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

   Type

   Blog Post

   Published

   Jan. 13, 2025, 9:49 p.m.

   ...guide Find CRISPR plasmids by function Browse our entire plasmid collection ...

10. Antibody Validation for Flow Cytometry

    Type

    Blog Post

    Published

    Sept. 10, 2024, 1:15 p.m.

    ...relative protein expression. Its reliability will be entirely dependent on the selectivity of the antibody used...

11. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type

    Blog Post

    Published

    Nov. 3, 2015, 3:30 p.m.

    ... Advanced functions: These functions are not entirely necessary but should be considered extremely useful...

12. Deep Dive: qPCR

    Type

    Blog Post

    Published

    Sept. 29, 2022, 1:15 p.m.

    ... you to measure the intensity of signal over an entire cycle. Since signals are produced by the presence...

13. 15 Hot Plasmids from 2017

    Type

    Blog Post

    Published

    Jan. 9, 2018, 3:02 p.m.

    ...identified as non-essential. The knockouts replace entire protein-coding regions (excluding start and stop...

14. Troubleshooting and Optimizing a Western Blot

    Type

    Blog Post

    Published

    Sept. 17, 2024, 1:15 p.m.

    ...migrate…so don’t try eliminating one component entirely. If you are trying to transfer proteins of vastly...