...second clean up step to remove the cleaved tag from solution. MBP antibodies are available for detection and...purification, be aware that the tag dimerizes in solution, which can affect downstream applications. Also...called polyhistidine tags, typically include six consecutive His residues (or more). Histidine bonds with ...

...into the clinic as well. The first sdAb based therapeutic was approved in 2018, with many others in clinical...clinical trials (Jin, et al. 2023). In addition to therapeutics, there is a lot of excitement around using sdAbs...Nanobodies: A Review of Generation, Diagnostics and Therapeutics. Int J Mol Sci 24:5994. https://doi.org/10.3390...

...information and, of course, plasmids. My primary duties are to manage this very blog (you’ll have to let...giving it a high five. While screaming can be therapeutic and I’ve certainly done my fair share of venting... about Management for Scienitsts from Addgene Executive Director Joanne Kamens ...

...relevant kinetic analysis, but the trick is to utilize a second immobilization method. One possibility...efficiency of biotinylation, at least in vitro. Our solution was to exploit the best of both worlds. We generated...folding (tested using real science). Make a stock solution of 2.5 mM biotin (50X) because 5 mM is barely ...

...on being financially self-sustaining through distributions of research materials, such as plasmids and ...new project to reach a sufficient number of distributions to cover costs, which can limit expansion into...” says Dr. Daniela Bourges, Addgene’s interim Executive Director. “NABOR provides a new model for the ...

...fluorescence-based quantification of base editors that utilize dCas9 fused to cytidine deaminases like APOBEC....mediated Editing (ACE) in real-time. The reporter constitutively expresses eGFP to indicate reporter abundance...quantify Cas9 cleavage, thus exhibiting its versatile utility in quantifying multiple gene editing activities...

... need to get started using plasmids and the revolutionary CRISPR technology in their own labs. We’re overjoyed...new technologies that will bring about the next revolution in biological research. We’re incredibly happy... how Blue-White screening could be a potential solution. 5. Cpf1: A New Tool for CRISPR Genome Engineering...

... plasmids: Twitch sensors Griesbeck’s recent contribution to the field is a series of fluorescence resonance...tonic action potential firing in neurons and high resolution functional tracking of T lymphocytes, too, making...sensitivity. He and his colleagues came up with a solution: they devised self-associating fluorescent domains...

...not convinced that your project is an innovative solution with a strong impact, or a journalist does not...taking a break and incorporating EAs into your routine.  Getting started: Planning your career path and...informational interviews to gain insight into the daily routine of the potential job Join LinkedIn and other ...

... antibodies. This is particularly useful for therapeutic applications in vivo where endogenous anti-AAV...anti-AAV antibodies often neutralize the therapeutic efficacy of AAV vectors. How are exosome AAVs produced...interest special contexts, such as the delivery of therapeutic proteins to the brain which requires crossing...

...generally agreed-upon international moratorium on utilizing CRISPR/Cas9 in humans. This topic was to be revisited... unethical experimentation on human subjects, utilizing a scientific technique not proven adequately safe... of the consulting firm Rogue Bioethics. Kelly utilizes her firsthand experience in the software industry...

... to track down that resource. A more long-term solution is the use of unique persistent identifiers (PIDs...CURIEs) combined with well-established prefix resolution services such as Identifiers.org and N2T.net....when citing, you can copy and paste the text attributing the PI who generated the plasmid, followed by...

... that can be targeted (hopefully by existing therapeutics that are safe, effective, and already FDA-approved...druggable) is a key step in our strategy to throw a therapeutic monkeywrench into the virus’ hijacking of human...partnering with Addgene, who will be taking over the distribution to expand the network of scientists working ...

...researchers are now using it to investigate potential therapeutics for complex genetic diseases. So we’re revisiting...suggest it might work well enough to consider as a therapeutic.” There are many fundamental questions about ...Application in Genetic Diseases: A Review.” Current pharmaceutical biotechnology. https://doi.org/10.2174/1389201021666200621161610...

...between researcher and expert; 4) Credit all contributions.   Our volunteers are extremely diverse....where a researcher makes a small but crucial contribution to someone else’s project. We think that these...right expert and the lack of credit for small contributions. We strive to eliminate these two barriers, ...

... this app is it turns your writing habit into beautiful graphs! I like to look at these graphs when I ... need that extra push to stick with my writing routine. The graph of which days of the week you most often...categorized by the goal of the phrase, such as being cautious, being critical, or defining terms. The phrases...

...systems are fast (often <10 minutes) and simple, utilizing individually packaged membrane ‘sandwiches’. They...proprietary hardware sold by the manufacturer. Blocking solutions Your blocking options come in two categories: ...enzymes cannot be used for multiplexing, and the solutions used to provide the chemilumescence may be light-sensitive... the past few years. Pro tip! You only need a resolution of ~150 µm to image a blot. This is because proteins...

...Mutagenesis is a tool that both evolution and molecular biologists use to tinker with DNA. Making changes...help scientists identify and/or facilitate the evolution of new phenotypes, and forward genetics harnesses...rate. For example the Phage Assisted Continuous Evolution (PACE) method requires constant turbidity of the...

...pandemic in which scientists race to find vaccines, therapeutics, and other treatments to stem the spread of ... accelerate research and to help find a faster solution to the COVID-19 pandemic. Though we’ve confirmed...citation to the plasmid page on our website. Contributing your results helps other researchers using these...

...cassettes typically contain a promoter (inducible or constitutive), your gene of interest, and often a selectable...your construct in target cells transiently or constitutively depending on the virus used. All of these ...libraries makes them the ideal vector for the job. Therapeutic applications Vaccine development Attenuated and...