We narrowed to 635 results for: UTY

.../Cas9 effector (LACE) system, the Gersbach lab utilized a similar strategy to develop an optimized photoactivatable...repair (HDR). This system is unique in that it utilizes a “split” nuclease - the authors fragmented Cas9...the figure below. Their most successful design utilized Magnet photoswitchable proteins derived from the...far-red light-activated split-Cas9 (FAST) system constitutively expresses the C-terminal fragment of Cas9 while...

... data. Here are some tips to help you in your execution:  Accuracy is not as important as precision for...need to run a validation assay in which serial dilutions for the target and housekeeping genes are run ...plotted against the log input concentration for each dilution. For the ΔΔCT method to be accurate, the absolute...Palais R.; Wittwer CT. uMELT: prediction of high-resolution melting curves and dynamic melting profiles of...

...more sustainable products Recycling is great for rerouting waste from the landfill, but what about all the...do. Remember, it was only 50 years ago that we routinely dumped toxic waste into the oceans, a practice...continue to examine our behavior and innovate solutions and practices to meet higher environmental standards...

... the lab. Whether you’re tired of calculating dilutions or you can’t quite remember the hazards of that..., Lianna Swanson, and researcher at Sanofi Pharmaceuticals. This post highlights a rare disease, Fibrodysplasia...and has huge potential for the development of therapeutics. It also represents a first for Addgene - while...

...impressive 1,350 scientists from more than 275 institutions all over the world. In the course of all those...community and contribute in a way that everyone could utilize what we had made,” Baird said. “It won’t sit in...scientific community," says Joanne Kamens, Addgene's Executive Director, "and to promote the great work of Michael...

...signal-to-background ratio, thus, allowing higher resolution in samples containing substantial autofluorescence...ultrasound waves as opposed to light to produce higher resolution images in vivo. “One of the reasons we can’t ... less. As a result, ultrasound enables higher resolution images at depths up to eight millimeters – not...

...fulfill our mission by generating revenue through distribution of research materials. Our small shipping and...generate revenue (as Addgene does through materials distribution). In fact, healthy nonprofits should have some...request plasmids (via web interface, processes, distribution, and customer service) Addgene provides unmatched...

...into the clinic as well. The first sdAb based therapeutic was approved in 2018, with many others in clinical...clinical trials (Jin, et al. 2023). In addition to therapeutics, there is a lot of excitement around using sdAbs...Nanobodies: A Review of Generation, Diagnostics and Therapeutics. Int J Mol Sci 24:5994. https://doi.org/10.3390...

...second clean up step to remove the cleaved tag from solution. MBP antibodies are available for detection and...purification, be aware that the tag dimerizes in solution, which can affect downstream applications. Also...called polyhistidine tags, typically include six consecutive His residues (or more). Histidine bonds with ...

...information and, of course, plasmids. My primary duties are to manage this very blog (you’ll have to let...giving it a high five. While screaming can be therapeutic and I’ve certainly done my fair share of venting... about Management for Scienitsts from Addgene Executive Director Joanne Kamens ...

...fluorescence-based quantification of base editors that utilize dCas9 fused to cytidine deaminases like APOBEC....mediated Editing (ACE) in real-time. The reporter constitutively expresses eGFP to indicate reporter abundance...quantify Cas9 cleavage, thus exhibiting its versatile utility in quantifying multiple gene editing activities...

...relevant kinetic analysis, but the trick is to utilize a second immobilization method. One possibility...efficiency of biotinylation, at least in vitro. Our solution was to exploit the best of both worlds. We generated...folding (tested using real science). Make a stock solution of 2.5 mM biotin (50X) because 5 mM is barely ...

...on being financially self-sustaining through distributions of research materials, such as plasmids and ...new project to reach a sufficient number of distributions to cover costs, which can limit expansion into...” says Dr. Daniela Bourges, Addgene’s interim Executive Director. “NABOR provides a new model for the ...

... need to get started using plasmids and the revolutionary CRISPR technology in their own labs. We’re overjoyed...new technologies that will bring about the next revolution in biological research. We’re incredibly happy... how Blue-White screening could be a potential solution. 5. Cpf1: A New Tool for CRISPR Genome Engineering...

... plasmids: Twitch sensors Griesbeck’s recent contribution to the field is a series of fluorescence resonance...tonic action potential firing in neurons and high resolution functional tracking of T lymphocytes, too, making...sensitivity. He and his colleagues came up with a solution: they devised self-associating fluorescent domains...

...not convinced that your project is an innovative solution with a strong impact, or a journalist does not...taking a break and incorporating EAs into your routine.  Getting started: Planning your career path and...informational interviews to gain insight into the daily routine of the potential job Join LinkedIn and other ...

... that can be targeted (hopefully by existing therapeutics that are safe, effective, and already FDA-approved...druggable) is a key step in our strategy to throw a therapeutic monkeywrench into the virus’ hijacking of human...partnering with Addgene, who will be taking over the distribution to expand the network of scientists working ...

...researchers are now using it to investigate potential therapeutics for complex genetic diseases. So we’re revisiting...suggest it might work well enough to consider as a therapeutic.” There are many fundamental questions about ...Application in Genetic Diseases: A Review.” Current pharmaceutical biotechnology. https://doi.org/10.2174/1389201021666200621161610...

...between researcher and expert; 4) Credit all contributions.   Our volunteers are extremely diverse....where a researcher makes a small but crucial contribution to someone else’s project. We think that these...right expert and the lack of credit for small contributions. We strive to eliminate these two barriers, ...

... this app is it turns your writing habit into beautiful graphs! I like to look at these graphs when I ... need that extra push to stick with my writing routine. The graph of which days of the week you most often...categorized by the goal of the phrase, such as being cautious, being critical, or defining terms. The phrases...