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We narrowed to 632 results for: Uty

Showing: 201 - 220 of 632 results

  1. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ... data. Here are some tips to help you in your execution:  Accuracy is not as important as precision for...need to run a validation assay in which serial dilutions for the target and housekeeping genes are run ...plotted against the log input concentration for each dilution. For the ΔΔCT method to be accurate, the absolute...Palais R.; Wittwer CT. uMELT: prediction of high-resolution melting curves and dynamic melting profiles of...

  2. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ...more sustainable products Recycling is great for rerouting waste from the landfill, but what about all the...do. Remember, it was only 50 years ago that we routinely dumped toxic waste into the oceans, a practice...continue to examine our behavior and innovate solutions and practices to meet higher environmental standards...

  3. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ... the lab. Whether you’re tired of calculating dilutions or you can’t quite remember the hazards of that..., Lianna Swanson, and researcher at Sanofi Pharmaceuticals. This post highlights a rare disease, Fibrodysplasia...and has huge potential for the development of therapeutics. It also represents a first for Addgene - while...

  4. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    Published
    July 29, 2014, 6:16 p.m.
    ...impressive 1,350 scientists from more than 275 institutions all over the world. In the course of all those...community and contribute in a way that everyone could utilize what we had made,” Baird said. “It won’t sit in...scientific community," says Joanne Kamens, Addgene's Executive Director, "and to promote the great work of Michael...

  5. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    Published
    March 27, 2014, 5:14 p.m.
    ...signal-to-background ratio, thus, allowing higher resolution in samples containing substantial autofluorescence...ultrasound waves as opposed to light to produce higher resolution images in vivo. “One of the reasons we can’t ... less. As a result, ultrasound enables higher resolution images at depths up to eight millimeters – not...

  6. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...fulfill our mission by generating revenue through distribution of research materials. Our small shipping and...generate revenue (as Addgene does through materials distribution). In fact, healthy nonprofits should have some...request plasmids (via web interface, processes, distribution, and customer service) Addgene provides unmatched...

  7. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...into the clinic as well. The first sdAb based therapeutic was approved in 2018, with many others in clinical...clinical trials (Jin, et al. 2023). In addition to therapeutics, there is a lot of excitement around using sdAbs...Nanobodies: A Review of Generation, Diagnostics and Therapeutics. Int J Mol Sci 24:5994. https://doi.org/10.3390...

  8. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...second clean up step to remove the cleaved tag from solution. MBP antibodies are available for detection and...purification, be aware that the tag dimerizes in solution, which can affect downstream applications. Also...called polyhistidine tags, typically include six consecutive His residues (or more). Histidine bonds with ...

  10. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...relevant kinetic analysis, but the trick is to utilize a second immobilization method. One possibility...efficiency of biotinylation, at least in vitro. Our solution was to exploit the best of both worlds. We generated...folding (tested using real science). Make a stock solution of 2.5 mM biotin (50X) because 5 mM is barely ...

  13. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ... need to get started using plasmids and the revolutionary CRISPR technology in their own labs. We’re overjoyed...new technologies that will bring about the next revolution in biological research. We’re incredibly happy... how Blue-White screening could be a potential solution. 5. Cpf1: A New Tool for CRISPR Genome Engineering...

  14. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ... plasmids: Twitch sensors Griesbeck’s recent contribution to the field is a series of fluorescence resonance...tonic action potential firing in neurons and high resolution functional tracking of T lymphocytes, too, making...sensitivity. He and his colleagues came up with a solution: they devised self-associating fluorescent domains...

  15. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...not convinced that your project is an innovative solution with a strong impact, or a journalist does not...taking a break and incorporating EAs into your routine.  Getting started: Planning your career path and...informational interviews to gain insight into the daily routine of the potential job Join LinkedIn and other ...

  16. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ... that can be targeted (hopefully by existing therapeutics that are safe, effective, and already FDA-approved...druggable) is a key step in our strategy to throw a therapeutic monkeywrench into the virus’ hijacking of human...partnering with Addgene, who will be taking over the distribution to expand the network of scientists working ...

  17. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    Published
    June 1, 2021, 1:15 p.m.
    ...between researcher and expert; 4) Credit all contributions.   Our volunteers are extremely diverse....where a researcher makes a small but crucial contribution to someone else’s project. We think that these...right expert and the lack of credit for small contributions. We strive to eliminate these two barriers, ...

  18. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ... this app is it turns your writing habit into beautiful graphs! I like to look at these graphs when I ... need that extra push to stick with my writing routine. The graph of which days of the week you most often...categorized by the goal of the phrase, such as being cautious, being critical, or defining terms. The phrases...

  19. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ... antibodies. This is particularly useful for therapeutic applications in vivo where endogenous anti-AAV...anti-AAV antibodies often neutralize the therapeutic efficacy of AAV vectors. How are exosome AAVs produced...interest special contexts, such as the delivery of therapeutic proteins to the brain which requires crossing...

  20. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...generally agreed-upon international moratorium on utilizing CRISPR/Cas9 in humans. This topic was to be revisited... unethical experimentation on human subjects, utilizing a scientific technique not proven adequately safe... of the consulting firm Rogue Bioethics. Kelly utilizes her firsthand experience in the software industry...
Showing: 201 - 220 of 632 results