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We narrowed to 643 results for: Vars

Showing: 201 - 220 of 643 results

  1. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...cilia. They’ll put a variety of plasmids to work to explore the effect of various OCRL1 mutations on cell... with CRISPR-Cas9. There are so many different variants and strategies out there. This will help us acquire...

  2. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...blogger Talley Lambert, a Research Associate at Harvard Medical School. The need for a community fluorescent...Joachim Goedhart on the Addgene blog – outlining various important considerations when choosing a FP, but...Lambert! Talley Lambert is a Research Associate at Harvard Medical School. He manages the Cell Biology Microscopy...

  3. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...never completely eliminate the risk that a minor variation in experimental conditions will derail a research...and why other publishers and organizations have various different versions of checklists and guidelines...Elsevier after her postdoctoral studies, and has held various editorial roles on review and primary research ...

  4. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...researchers have been able to study the effects of various stimuli on global translation, but a visual technique...second. They also explored the effects of 5’ UTR variants in translation of cell cycle protein Emi1, showing...represent powerful tools for studying translation in various cell types and physiological states. References...

  5. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    Published
    July 27, 2017, 1:11 p.m.
    ...used N- and C-terminal dronpa fusions. With these various designs, Xin has been able to develop photoswitchable...has demonstrated the utility of these FLiPs in a variety of research applications and particularly notes...be able to use similar designs to create a wide variety of enzymes and proteins with inducible activity...

  6. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...resistant to detergents and degradation under a variety of conditions. They are composed of 4 proteins ...robust linear actuators that could be used in a variety of applications. For example, you could use them...Silver in the department of Systems Biology at Harvard Medical School. Follow her on Twitter @jessicapolka...

  7. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...Institute today, where he helps researchers in a variety of fields develop RNAi screens to answer their ...more about cancer biology in Ed Harlow’s group at Harvard Medical School and then applied to work at the ...Genetic Perturbation Platform and encompasses a variety of technologies to functionally investigate the...

  8. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...London), Dr. Connie Cepko (Harvard Medical School) , and Dr. George Church (Harvard Medical School) for taking...and writer.” Connie Cepko was surprised by the variety of skill sets she needed to acquire as a professor...

  9. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...London), Dr. Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) for taking...throughout the year since hiring opportunities arise at various times. If the timing and interests align for both...

  11. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...degenerate primer set to amplify IgG variable light (VL) and variable heavy (VH) chain sequences. Fusion...thoroughly validated. The creativity in employing a variety of cloning techniques and troubleshooting methodologies...

  12. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ... there!), think of ways you could change your variables - your approach, your workflow, or your physical...prevent the mistake, just like you would change variables to fix a failed experiment.  For example, I used...to reduce the chances of making an error. Try a variety of things, including (and maybe especially) suggestions...

  13. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...scale by screening diverse libraries of genetic variants. Several methods for generating mutant libraries...provides a new approach for generating novel genetic variants in bacteria. Read on to learn about the key components... other three bases, which limits the level of variation that can be introduced.   Diversification is...

  14. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...pressure to select for variants of interest, though. A selection can take the form of varied temperature, nutrient...help determine which is right for you. Screen variations Forward and reverse screens Forward and reverse...

  15. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...continuation of that work, they have found at least two variants that display increased neuronal expression in ...side effects, by allowing the user to select for variants that infect the CNS while selecting against those...subclones within a single well reduced well-to-well variability, while the readout was able to be deconvoluted...

  16. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...tens of thousands of proteins functioning in a variety of ways. How do scientists focus on the activity...acid sequence, or tag, on the protein. While a variety of methods can be used to successfully isolate ...tools for studying proteins and are used for a variety of assays in the lab (for a refresher see Antibodies...

  17. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...model, ubi:zebrabow. In this system, cells express various fluorescent proteins controlled by Cre recombination... that recombine independently and results in a variety of colors depending on the combination of fluorescent... Various Addgenies...

  18. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ... application. Consider also that there may be variations within an application. Take immunohistochemistry...might find that while the website highlights a variation of your application, a publication successfully...this is the case, you can narrow them down in a variety of ways. First, review the citations. An antibody...

  19. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...editing in both bacteria and human cells using various delivery methods, including plasmid, ribonucleoprotein...efficiency of prime editing to correct mutations in a variety of target genes and cell types. Their Nature Biotechnology... Various Addgenies...
Showing: 201 - 220 of 643 results