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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 642 results for: Vars;

Showing: 201 - 220 of 642 results

  1. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...cilia. They’ll put a variety of plasmids to work to explore the effect of various OCRL1 mutations on cell... with CRISPR-Cas9. There are so many different variants and strategies out there. This will help us acquire...

  2. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...blogger Talley Lambert, a Research Associate at Harvard Medical School. The need for a community fluorescent...Joachim Goedhart on the Addgene blog – outlining various important considerations when choosing a FP, but...Lambert! Talley Lambert is a Research Associate at Harvard Medical School. He manages the Cell Biology Microscopy...

  3. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...researchers have been able to study the effects of various stimuli on global translation, but a visual technique...second. They also explored the effects of 5’ UTR variants in translation of cell cycle protein Emi1, showing...represent powerful tools for studying translation in various cell types and physiological states. References...

  4. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    Published
    July 27, 2017, 1:11 p.m.
    ...used N- and C-terminal dronpa fusions. With these various designs, Xin has been able to develop photoswitchable...has demonstrated the utility of these FLiPs in a variety of research applications and particularly notes...be able to use similar designs to create a wide variety of enzymes and proteins with inducible activity...

  5. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...never completely eliminate the risk that a minor variation in experimental conditions will derail a research...and why other publishers and organizations have various different versions of checklists and guidelines...Elsevier after her postdoctoral studies, and has held various editorial roles on review and primary research ...

  6. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...resistant to detergents and degradation under a variety of conditions. They are composed of 4 proteins ...robust linear actuators that could be used in a variety of applications. For example, you could use them...Silver in the department of Systems Biology at Harvard Medical School. Follow her on Twitter @jessicapolka...

  7. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...Institute today, where he helps researchers in a variety of fields develop RNAi screens to answer their ...more about cancer biology in Ed Harlow’s group at Harvard Medical School and then applied to work at the ...Genetic Perturbation Platform and encompasses a variety of technologies to functionally investigate the...

  8. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...London), Dr. Connie Cepko (Harvard Medical School) , and Dr. George Church (Harvard Medical School) for taking...and writer.” Connie Cepko was surprised by the variety of skill sets she needed to acquire as a professor...

  9. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...London), Dr. Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) for taking...throughout the year since hiring opportunities arise at various times. If the timing and interests align for both...

  10. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...pressure to select for variants of interest, though. A selection can take the form of varied temperature, nutrient...help determine which is right for you. Screen variations Forward and reverse screens Forward and reverse...

  11. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...continuation of that work, they have found at least two variants that display increased neuronal expression in ...side effects, by allowing the user to select for variants that infect the CNS while selecting against those...subclones within a single well reduced well-to-well variability, while the readout was able to be deconvoluted...

  12. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...degenerate primer set to amplify IgG variable light (VL) and variable heavy (VH) chain sequences. Fusion...thoroughly validated. The creativity in employing a variety of cloning techniques and troubleshooting methodologies...

  13. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ... there!), think of ways you could change your variables - your approach, your workflow, or your physical...prevent the mistake, just like you would change variables to fix a failed experiment.  For example, I used...to reduce the chances of making an error. Try a variety of things, including (and maybe especially) suggestions...

  15. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...scale by screening diverse libraries of genetic variants. Several methods for generating mutant libraries...provides a new approach for generating novel genetic variants in bacteria. Read on to learn about the key components... other three bases, which limits the level of variation that can be introduced.   Diversification is...

  16. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ... is explained in more detail here. There are a variety of methods that can be used to measure your fluorescent...filtration (Chudakov et al, 2007). We have developed a variety of plasmids that can be used in the OSER assay ... Of course, you want the ‘latest and greatest’ variants with the best properties for your research. But...

  17. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...different fluorescence imaging strategies use widely varying amounts of excitation power (Shaner et al, 2008... An example of a photostability measurement of various cyan fluorescent proteins is depicted in figure...higher excitation power, the photostability of the variants is comparable (lower panel of figure 1). To avoid...

  18. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...RNA-protein interactions. To address this need, the Khavari lab at Stanford created the RNA-protein interaction...disease. RaPID can also be used to test how RNA variants associated with human disease affect protein binding...Siprashvili, Z., Palmer, T.D., Carette, J.E., & Khavari, P.A. (2018). RNA–protein interaction detection...

  19. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...tens of thousands of proteins functioning in a variety of ways. How do scientists focus on the activity...acid sequence, or tag, on the protein. While a variety of methods can be used to successfully isolate ...tools for studying proteins and are used for a variety of assays in the lab (for a refresher see Antibodies...

  20. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...from countless rounds of optimization. Dozens of variants exist with unique cutting features and evolved...engineering endeavors. For more information on Cas9 variants, check out our blog post on choosing a Cas9 enzyme... detection tool for rare, often hard to detect variants such as single nucleotide polymorphisms. Fast...
Showing: 201 - 220 of 642 results