... ITR sequences from different AAV serotypes that vary in their RBE and T-shaped hairpin loop sequences...non-AAV2 ITRs can be used to drive gene expression at varying capacities. AAV vectors containing the AAV2 ITRs...different combinations of ITR and capsid serotypes vary in their gene transfer efficiency. Our practical...

...resistant to detergents and degradation under a variety of conditions. They are composed of 4 proteins ...robust linear actuators that could be used in a variety of applications. For example, you could use them...Silver in the department of Systems Biology at Harvard Medical School. Follow her on Twitter @jessicapolka...

...Institute today, where he helps researchers in a variety of fields develop RNAi screens to answer their ...more about cancer biology in Ed Harlow’s group at Harvard Medical School and then applied to work at the ...Genetic Perturbation Platform and encompasses a variety of technologies to functionally investigate the...

...reporter, other luciferases have been identified in various species with differing kinetics, substrate requirements...plagiophthalmus), railroad worm (Phrixothrix hirtus), and a variety of marine crustaceans. Many of these luciferases...firefly(Cypridina hilgendorfii) 465 62 Cypridina (Vargulin) luciferin None Yes Sea pansy(Renilla reniformis...

...London), Dr. Connie Cepko (Harvard Medical School) , and Dr. George Church (Harvard Medical School) for taking...and writer.” Connie Cepko was surprised by the variety of skill sets she needed to acquire as a professor...

...London), Dr. Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) for taking...throughout the year since hiring opportunities arise at various times. If the timing and interests align for both...

...mammalian expression vectors containing various spike protein variants are also available. Plasmids are continually... Various Addgenies...

...scale by screening diverse libraries of genetic variants. Several methods for generating mutant libraries...provides a new approach for generating novel genetic variants in bacteria. Read on to learn about the key components... other three bases, which limits the level of variation that can be introduced.   Diversification is...

...pressure to select for variants of interest, though. A selection can take the form of varied temperature, nutrient...help determine which is right for you. Screen variations Forward and reverse screens Forward and reverse...

...continuation of that work, they have found at least two variants that display increased neuronal expression in ...side effects, by allowing the user to select for variants that infect the CNS while selecting against those...subclones within a single well reduced well-to-well variability, while the readout was able to be deconvoluted...

...degenerate primer set to amplify IgG variable light (VL) and variable heavy (VH) chain sequences. Fusion...thoroughly validated. The creativity in employing a variety of cloning techniques and troubleshooting methodologies...

... there!), think of ways you could change your variables - your approach, your workflow, or your physical...prevent the mistake, just like you would change variables to fix a failed experiment.  For example, I used...to reduce the chances of making an error. Try a variety of things, including (and maybe especially) suggestions...

...RNA-protein interactions. To address this need, the Khavari lab at Stanford created the RNA-protein interaction...disease. RaPID can also be used to test how RNA variants associated with human disease affect protein binding...Siprashvili, Z., Palmer, T.D., Carette, J.E., & Khavari, P.A. (2018). RNA–protein interaction detection...

... is explained in more detail here. There are a variety of methods that can be used to measure your fluorescent...filtration (Chudakov et al, 2007). We have developed a variety of plasmids that can be used in the OSER assay ... Of course, you want the ‘latest and greatest’ variants with the best properties for your research. But...

...different fluorescence imaging strategies use widely varying amounts of excitation power (Shaner et al, 2008... An example of a photostability measurement of various cyan fluorescent proteins is depicted in figure...higher excitation power, the photostability of the variants is comparable (lower panel of figure 1). To avoid...

...model, ubi:zebrabow. In this system, cells express various fluorescent proteins controlled by Cre recombination... that recombine independently and results in a variety of colors depending on the combination of fluorescent... Various Addgenies...

...tens of thousands of proteins functioning in a variety of ways. How do scientists focus on the activity...acid sequence, or tag, on the protein. While a variety of methods can be used to successfully isolate ...tools for studying proteins and are used for a variety of assays in the lab (for a refresher see Antibodies...

...from countless rounds of optimization. Dozens of variants exist with unique cutting features and evolved...engineering endeavors. For more information on Cas9 variants, check out our blog post on choosing a Cas9 enzyme... detection tool for rare, often hard to detect variants such as single nucleotide polymorphisms. Fast...

... application. Consider also that there may be variations within an application. Take immunohistochemistry...might find that while the website highlights a variation of your application, a publication successfully...this is the case, you can narrow them down in a variety of ways. First, review the citations. An antibody...

...editing in both bacteria and human cells using various delivery methods, including plasmid, ribonucleoprotein...efficiency of prime editing to correct mutations in a variety of target genes and cell types. Their Nature Biotechnology... Various Addgenies...