We narrowed to 460 results for: abo.3

...be controlled by blue light (Bubeck et al., 2018). 3. Reduce cytotoxicity of CRISPR-Cas editing in tissues...Disabling Cas9 by an anti-CRISPR DNA mimic. Sci Adv 3:e1701620 . https://doi.org/10.1126/sciadv.1701620 ...Bondy-Denomy et al., 2018). For example, AcrIIA4 mentioned above indicates that it’s the fourth Acr protein discovered...Cas9 Variant for Your Next Experiment Read more about Optogenetic Control of CRISPR   Resources on Addgene.org...

...Ras pathway. The term “Ras” collectively refers to 3 proteins (H-Ras, K-Ras, and N-Ras) encoded by a family...cell 25.3 (2014): 272-281. PubMed PMID: 24651010. 3. Ledford, Heidi. "The ras renaissance." Nature 520.7547... cancer are yielding promising results in the laboratory, and Addgene is accelerating this research by...within that pathway that are of interest to their laboratory. A single mouse click on a gene of interest will...

...encapsulating the protein of interest (Figure 3).   Figure 3: Schematic representation of the MPyV system...acid, suggesting that MPyV system can improve metabolic pathway yields by encapsulating a single enzyme...

...  Chromoproteins for allelic exchange Figure 3: pTOX plasmids expressing amilCP and tsPurple genes...genetic modification integrates into the genome (Figure 3). Here, the chromoprotein is expressed and gives the... Fluorescent Protein 101 blog series Learn more about FRET Find protocols and tips for making plasmid ...

... Consulting firms deploy case teams, usually with 3-6 consultants, who work on analyzing the situation...the client to prioritize investments over the next 3, 5, or 10 years. Complicating these challenges is ...company to collaborate whenever it faces an important challenge. The types of projects mentioned above are just...and why you might enjoy doing it. I’ll also talk about the skills you’ll need to do well as a healthcare...often work with such teams to help make decisions about R&D investments in either new internal efforts, ..., or through acquisitions of or collaborations with other companies. Medical Affairs groups work on building...building awareness among physicians and scientists about clinical research, drugs, the diseases they can ...

...into Stage 3 or Stage 4 cancer. These later stages of a cancer diagnosis indicate, for Stage 3, that the...common cancers like lung (about 224,000 diagnosed this year) or breast cancer (about 246,000 diagnosed this...Mesothelioma Cancer Alliance. We have learned much about the causes of cancer and the different avenues that... asbestos exposure to the time of diagnosis) is about 35 years, but it usually ranges from 20-50 years... an orphan cancer; it is rare disease with only about 3,000 new mesothelioma patients diagnosed every ...from mesothelioma several years ago – recently collaborated with Senator Barbara Boxer to introduce the ...Additional Resources on the Addgene Blog Find out More About Cancer and Addgene's Cancer Collection Pages Learn...

... dangle the carrot (or more popularly- cookies). 3. Do something outside of your ordinary environment...Utilize your office space too Balance point number 3 with doing fun things within your office. This way...early employees spent this remaining time by elaborately pranking each other, dressing in costume, and...frequently ask the question, “What’s your favorite part about working at Addgene?” Most Addgenies reply with an... confident that their teammates and project collaborators have completed their work thoroughly. It’s kind...rose-colored-glasses-esque picture of how everyone is kind and collaborative and all we do is have fun all day long, whistling...megaphone, or your company’s intranet. Don’t forget about the power of simple word of mouth - be sure to bring...

...the organization(s) you serve time and resources. 3. Get a mentor, find your people Find someone who is...GPCI evaluates a candidate's expertise (a minimum of 3 years experience) and experience through the candidate...on becoming grant writers. When we asked others about how they became grant professionals, mid- to late-career...late-career grant professionals have similar stories about a time when their passion for an organization or... logic models, established partnerships and collaborations, and commitments to and proof of compliance...grants. It is no surprise that the U.S. Bureau of Labor and Statistics shows that employment of grant writers... skills & passion  Skills such as research, collaboration across multiple disciplines, writing, project...

... for an extended period of time (>10 passages or >3 months). Other times cell lines are unavailable from...State University of New York Press, Albany (1986). 3. Masters, John R. "HeLa cells 50 years on: the good...Learn All about Mammalian Vectors Read up on CRISPR Delivery Systems for Mammalian Cells Learn about the Dangers...Misidentified or cross-contaminated cells have been known about since 1966 when Stanley Gartler reported that most...there is a loss of reputation that can impede collaborations, especially with industry. Today we also have...The LGC Group also provides standards and has laboratories to carry our independent quality control for...culture contamination." Archives of pathology & laboratory medicine 133.9 (2009): 1463-1467. PubMed PMID...

...interfere with it in a BSL-2 lab instead of a BSL-3 lab. Jesse Bloom’s lab published a protocol and reagents... CRISPR-Based Detection Tools. The CRISPR Journal 3:142–145. https://doi.org/10.1089/crispr.2020.0030 ...different SARS-CoV-2 spike variants. You can learn more about their research in this article. To see other plasmids... optimized. Browse the collection or learn more about it on the blog. COVID-19 research is happening fast...through plasmid sharing and more. As you’ve read above, some of these plasmids have been in the repository...Additional resources on the Addgene blog Learn more about mapping the SARS-CoV-2 - human protein-protein interaction... interaction Find tips for remote collaborations Browse all COVID-19 blog posts Resources on Addgene....

...enhancers 3. CRISPR out the enhancers from step 2. See which ESCs die or differentiate Step 3 reveals which...republished here with permission. Quilt is a collaborative database for genomics. In this article, Quilt...tri_methylation In the next section we’ll apply the above formula to a real-world experiment. We’ll start ...discovery and therapeutics. We’ll have more to say about clinical applications of CRISPR in the next section...Aneesh Karve is CTO at Quilt Data. Quilt is a collaborative database. Aneesh's research interests span machine...on Your CRISPR Background with CRISPR 101 Learn about CRISPR Delivery Methods Validate Your Genome Edit...

...type and the specific genes cloned into the vector.3  2A Peptides To overcome some of the disadvantages...et al. (J Virol. 1988 Aug;62(8):2636-43.) PubMed. 3. Highly Efficient Multicistronic Lentiviral Vectorswith...genes above with your gene or genes of interest. Some of the plasmids listed in the table above (and their... an internal region of the mRNA. In the figure above, you can see that the IRES element acts as another... first discovered in picornaviruses, are short (about 20 amino acids) and produce equimolar levels of ...

...Featured Topic Page Learn about Non-Homologous End Joining Learn about Base Editing Read 3 Tips to Improve HDR...pathway (Charpentier et al., Nat Com).     Fig. 3: HDR events compete with NHEJ-mediated edits of small... post on increasing HDR efficiency to read more about optimizing this distance. Homology arms and types...screening strategies for your experiment. Also learn about Addgene’s knock-in plasmids for reagents to help...

... Listen to Our Segment on Magneto 2.0 Hot Plasmid 3: The Human Kinase Domain Constructs Kit from John ...this new way to learn about plasmid technologies. If you have any comments about the Hot Plasmids Podcast...with these efforts, you’ll find new ways to learn about science careers, lab protocols, and, of course, ...each one, plug in some headphones to learn more about the latest and greatest plasmids through this podcast...

...genes which help mediate AAV viral replication; and 3) the transfer plasmid which encodes a transgene of...ITR-containing plasmid. This whole process takes ~3 hours to complete: 1 hours hands-on time and 2 hours...a workspace with plasmid, not that I would know about this… A no template control (NTC) should always ... variability. Check out this post to learn more about different ways to titer AAV. Do you have any AAV...at Fred Hutchinson Cancer Center for teaching me about AAV production and purification! References 1. Aurnhammer...

...expressing 1) FLAG-biotin-tagged dCas9, 2) BirA, and 3) one or more gRNAs Two separate lentiviruses delivering...characterize the ‘social networks’ of 157 super enhancers.  3. Measuring temporal changes in chromatin interactions...with current 3C methods provide useful information about chromatin interactions, they are grainy so it’s ...interactions opens the door for answering more questions about chromatin conformations. The Xu lab presented three...Cas9 Variant for Your Next Experiment Learn More About Designing gRNAs Against Your Favorite CREs Here ...

...with a single insertion because it will segregate 3:1 in the T2 generation. You also have no control over...PMID: 17087829. PubMed Central PMCID: PMC1636043. 3. Kyndt, T. et al. (2015) ‘The genome of cultivated... J. (2002) Arabidopsis : a laboratory manual. Cold Spring Harbor Laboratory Press. Available at: https...https://books.google.at/books/about/Arabidopsis.html?id=IfZAMNPWVk4C&redir_esc=y (Accessed: 21 September ...

...PMID 25282355. PubMed Central: PMCID: PMC4317352. 3. Beisel, Chase L., Ahmed A. Gomaa, and Rodolphe Barrangou...Targeted Bacterial Population Editing.” Cell Syst. 1(3):187-196. (2015). PubMed: PMID 26973885. Additional...organizations are working to help educate the public about proper antibiotic usage. Although these steps are...laudable, they likely won’t solve the growing problem. Above all, we need new antimicrobials, ideally ones for...used to deliver any of the three systems discussed above, with the caveat that phages are very diverse, and...

...introduced mutations that drive leukemia (Figure 3). Figure 3: Using LARRYv2 libraries for state-fate...Check out our blog post on AAV serotypes for more about the tissue tropism of these and other AAVs. ...β-secretase; AF = AMPA.FingR; FingR = fibronectin intrabody generated with mRNA display. Modified from Rivera...

... kb at most - which means that a gene larger than 3 kb cannot be delivered using this vector. For example...emerges from disgrace to be the next big thing, again 3. Acland G. et al., 2001. Gene therapy restores vision...famously visited Congress to help increase awareness about the potential of gene therapy!). It would take another...muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway and reported results...