Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 340 results for: abo.4

Showing: 201 - 220 of 340 results

  1. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...the Syntaxin3 gene was the winner. We used the next 4-5 weeks to work on cloning the Syntaxin3 gene in a...intern coordinator shortly after hearing her talk about Addgene’s internship opportunity taking place next...

  2. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ... OR you can store the reaction at -20C overnight. 4. Transform TOPO Cloning Reaction into Competent Cells...in mind that Taq polymerase has an error rate of about 1 in 3,500 bases. Typically polymerases with proofreading...

  3. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...Fact 19. https://doi.org/10.1186/s12934-020-01321-4   Kolodner R (1980) Genetic recombination of bacterial...molecular biology and viral vector protocols Learn about the common lab E. coli strains Find tips for viral...

  4. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ..., Johansson M. Nat Biotechnol. 2001 Aug;19(8):713-4. PubMed.  ... a protocol has been established) may be less laborious than viral purification, and it does not raise...

  5. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...Translation of Single mRNA Molecules In Vivo.” Cell 165(4) (2016): 976-89. PMID: 27153498 PMCID: PMC4889334 ...Additional Resources the Addgene Blog Learn More about SunTag Catch up on Your GFP Background Choose the...

  6. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ... Rhodamine / Cy5 (e.g. Semrock's) and the similar 4-laser combination on a confocal (405 / 488 / 561 /...received his PhD in biophysics from UCSF in the laboratory of Ronald Vale, after which he was a fellow at...

  8. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ..., vol. 5, no. 1, American Chemical Society (ACS), 4 Nov. 2015, pp. 99–103. Crossref, https://doi.org/10.1021... Multipart Assembly.” ACS Synthetic Biology, vol. 4, no. 9, American Chemical Society (ACS), 1 May 2015... constructs that compose genetic circuits and metabolic pathways. For many MoClo systems, the individual...interested in recombinant protein purification and metabolic engineering. If instead you want to build genetic...designed for flexibility. The Fragmid kit described above works in both mammalian systems and Drosophila. ... Signaling in CAR T Cells. Cold Spring Harbor Laboratory, 17 Feb. 2024. Crossref, https://doi.org/10.1101...Emerging CRISPR Technologies. Cold Spring Harbor Laboratory, 27 Oct. 2023. Crossref, https://doi.org/10.1016...

  9. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...chromophore, resulting in emission of red fluorescence (4). The dimeric or tandem dimer versions of EosFP are...biology 12.3 (2005): 279-285. PubMed PMID:15797211. 4.Wiedenmann, Jörg, et al. "EosFP, a fluorescent marker...emitted, making it easier to capture a clear image above background. The majority of PA-FPs come in 2 flavours...Additional Resources on the Addgene Blog Read More about Fluorescent Protein Imaging Choosing Your Fluorescent...

  10. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...Something in their world should be fast and affordable. 4. Stem cell scientists are generous Besides some of...the Addgene Blog Learn about Efforts to Fight Rare disease with iPSCs Read About Addgene's Outreach Efforts...the first clinical trial with iPSCs with his collaborator Takahashi in 2014 – just 8 years after the OSKM...researchers special: 1. Stem cell scientists care deeply about ethics Ethics must play a prominent role in the ...Researcher Darrell Kotton 2. Stem cell scientists care about patients As any grad student who has spent years... a big picture perspective: Dr. Yamanaka talked about his clinical trial patients with advanced macular...proof that these researchers are thriving in a collaborative, rather than a combative, field. Of course they...

  11. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...be a major decision. A science trainee will spend 4-7 years working for one person and with a group who...during the day? We asked three lab heads how they go about selecting new hires. This is followed by some perhaps...visits the lab for a full day, gives a 1 hour talk about his or her interests, and spends one-on-one time...current members to share short-term goals and chat about awesome science. Important factors in the search..., the lab has a reputation for not entering collaborations. Alternatively, perhaps the lab publishes and...Medical School) for taking the time to speak with us about adding new scientists to their labs. Read the Other...

  12. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...restriction enzyme sequence resource is universally useful. 4. Chemical Safety Data Sheets by ThatsMyStapler Safety...saved and cannot be deleted, so there is no worry about misplacing your results or losing that lab notebook...enzyme, or name to discover all the information about that restriction enzyme or share it via email with... this app. However, if you get quizzed randomly about a hazardous chemical and can’t find your lab’s MSDS... new researchers or students beginning to learn about biochemistry. The least this app will do is allow...

  13. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...recombination. In most recombineering strategies, only 3-4 phage proteins are needed and these can be provided...from previously discussed methods. As described above, you design two primers, each containing the desired...suited for insertions and deletions. The figure above shows guidelines for primer design; a desired insertion...mutation-containing colonies/total colonies) is about 84% for each of the three methods. However, REPLACR-mutagenesis... Additional Resources on the Addgene Blog Read about Other Plasmid Cloning Techniques like SLIC and Golden...

  14. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...Obligate aerobe Number of chromosomes 16 3 4 Preferred carbon source Glucose Glucose ...pombe have dominated the research scene. But what about the other yeasts – in the biomedical world, are ...pharmaceutical companies (along with some research laboratories) have picked up the system for some of the uses...eukaryotic cells and peroxisomes are where methanol metabolism occurs in methylotrophic organisms. K. phaffii...

  15. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...mediated DNA damage is a DSB within the target DNA ~3-4 nucleotides upstream of the PAM sequence. Cas9 Species...that you know about all the different components of CRISPR, It's time to start thinking about how you can...Use the following flow chart to get you thinking about the types of experiments you're planning on doing...

  16. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...transcriptional activation screening. Nat Protoc. 2017 (4):828-863. doi: 10.1038/nprot.2017.016. Additional...changes. Using this road map, and what was known about previously characterized TFs, the Zhang group were...Additional Resources on the Addgene Blog Learn More about Lentivirus Figure out How to Get the Most from Your...

  17. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ... 83897 AAVrg pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Controls Plasmid Serotype Name 114471 AAV2...vector name and serotype you’d like to be notified about. Chemogenetics Plasmid Serotype Name 44362 ...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

  18. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...experiment, consider hfYFP and LSSmGFP.     Fig. 4: hfYFP retains folding and fluorescence in high molarity... minutes of FUS.  Similar constructs to the one above can be made for expressing your favorite T-cell ...

  19. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...Environmental Microbiology  1.6%  1.2% 0.75   2.3 4 2010 167  Applied and Environmental Microbiology... with plasmids deposited at Addgene – typically about four times as many as papers without plasmids deposited...

  20. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...https://doi.org/10.2174/1389201021666200621161610 4. Uddin et al. 2020.  “CRISPR Gene Therapy: Applications...therapeutic.” There are many fundamental questions about CRISPR/Cas biology still left to answer as well,...
Showing: 201 - 220 of 340 results