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We narrowed to 407 results for: abo.4

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Showing: 1 - 20 of 407 results

  1. Immunology Research Plasmids and Resources

    Type
    Collection
    ...heavy diversity 4-23 (non-functional) IGHD423 IGHD4-4 immunoglobulin heavy diversity 4-4 DA4, IGHD44 IGHD5...heavy variable 4-30-2 IGHV4-3, IGHV4302 IGHV4-30-4 immunoglobulin heavy variable 4-30-4 IGHV4-3, IGHV4304...immunoglobulin heavy variable 4-39 IGHV439, VH IGHV4-4 immunoglobulin heavy variable 4-4 IGHV44, VH IGHV4-59 immunoglobulin...104A BD-4, DEFB-4, DEFB104, DEFB4, MGC118942, MGC118944, MGC118945, hBD-4 DEFB4 defensin, beta 4 DEFB-2...lambda variable 4-3 IGLV43, V5-1 IGLV4-60 immunoglobulin lambda variable 4-60 IGLV460, V5-4 IGLV4-69 immunoglobulin...hIL-3Ra IL4 interleukin 4 BCGF-1, BCGF1, BSF1, IL-4, MGC79402 IL4R interleukin 4 receptor CD124, IL4RA ...

  2. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...2023 May 4. Kaspar Podgorski Glutamate FRET sensor to monitor glutamate transport and metabolism (FLIPE)...single-cell resolution. PLoS Biol. 2025 Apr 29;23(4):e3003048. Kiryl Piatkevich Calcium Fast red fluorescent...tetramethylrhodamine-labeled ParM. ACS Chem Biol. 2010 Apr 16;5(4):415-25. Martin Webb ATP Multicolor palette of ATP...Dynamics. Cell. 2020 Nov 17. pii: S0092-8674(20)31399-4. Edward Boyden cAMP (cyclic AMP) FRET sensors of cAMP... and with high affinity. PLoS One. 2015 Apr 14;10(4):e0122513. Kees Jalink cAMP (cyclic AMP) Fluorescent... chloride in neuronal processes. PLoS One. 2012;7(4):e35373. Susan Amara , Susan Ingram Citrate Nanosensors...Single beta Cells. Cell Chem Biol. 2017 Apr 20;24(4):525-531.e4. Dmytro Yushchenko Lactate Laconic FRET-based...

  3. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ... miRFP703 673 703 8 pmiRFP703-N1 miRFP709 683 709 4 pmiRFP709-N1 miRFP713 690 713 7 pmiRFP713-N1 iRFP ...Viral Vector Packaging Service Content last reviewed: 4 December 2025 Do you have suggestions for other plasmids...in live animals are those with emission near or above 650 nm (far-red or near-infrared), as signals below...imaging of tissues in living animals. Learn more about in vivo imaging tools in this blog post . Far-Red...

  4. Antibody Guide

    Type
    Collection
    ...small multiplex experiments 4-color flow panel: BV421, FITC, PE, and APC 4-color microscopy panel: DAPI...protects mucosal membranes Dimer with a valency of 4 IgA1; IgA2 IgD Coexpressed with IgM on the surface... As a guideline, most antibodies can be stored at 4 °C for short-term storage. For long-term storage they...conjugated to fluorophores should never be stored below 4 °C. Certain isotype subclasses, such as IgG3, aggregate...aggregate after thawing and therefore should be kept at 4 °C. Record and track lot numbers for all antibodies...typically use HRP as a signaling molecule. Figure 4: A direct sandwich ELISA, which uses a capture antibody...are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments, each recognizing...

  5. AAV Packaged on Request

    Type
    Collection
    ...Typical Titer Small NEW 2 × 100 µL aliquots 0.2 mL 4 × 10 12 GC/mL* 1 × 10 13 GC/mL* Medium 10 × 100 µL...scientist through an MTA. This process typically takes 1–4 days, depending on your institution. AAV Production...information before choosing a serotype? Learn more about AAV serotypes and tissue tropism on Addgene's blog...Additional Resources Viral Vector Production Learn about how we produce high-quality viral vector preps at...

  6. CRISPR Guide

    Type
    Collection
    ...double-strand break (DSB) within the target DNA, located ∼3–4 nucleotides upstream of the PAM sequence. The resulting... , or others, can increase HDR efficiency. Figure 4: Overview of HDR. In most applications, a large portion... for In Vivo Genome Editing. Molecular Cell , 73 (4), 714-726.e4. PMID: 30581144 Fu, Y., Foden, J. A.,...engineered CjCas9 orthologs. Molecular Therapy , 31 (4), 1177–1187. PMID: 36733251 Harrington, L. B., Doxzen...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman,...CRISPR/CAS-Mediated Genome Engineering. Cell , 153 (4), 910–918. PMID: 23643243 Zhang, Y., & Marchisio, ...cytidine deaminase fusions. Nature Biotechnology . 35 (4), 371–376. PMID: 28191901 Komor, A. C., Kim, Y. B....

  7. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... pKa Maturation Structure Plasmids Sirius 355 424 4 3.0 Prone to dimerization Sirius-N1 - Mammalian Expression...mKate1.31-pBAD - Bacterial Expression mPlum 590 649 4 4.5 100 min Monomer pQC mPlum IX - Mammalian Expression...mRaspberry-N1 - Mammalian Expression TagRFP675 598 675 4 5 Prone to dimerization pTagRFP675-N1 - Mammalian ...His-miRFP670 - Bacterial Expression iRFP670 643 670 13 4 ~5 h Dimer piRFP670-N1 - Mammalian Expression pBAD...pemiRFP703-N1 - Mammalian Expression miRFP709 683 709 4 4.5 Monomer pmiRFP709-N1 - Mammalian Expression pBAD...colors as needed for your experiments. Learn more about the flexibility of self-labeling tags on our blog...

  8. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...Request service, Addgene guarantees a titer of at least 4 x 10 12 GC/mL for the AAV1, AAV5, AAV6, AAV8, AAV11... by email will supersede the general guarantees above. We will have our expert viral vector production...supersede the general estimated turnaround time above. After starting viral production, if we determine...

  9. COVID-19 Resources

    Type
    Collection
    ...al., 2014). PCP4 plasmids - (Purkinje cell protein 4) is involved in beating of airway cilia (Kogiso et...proximal origin of SARS-CoV-2 . Nature medicine, 26 (4), 450–452. https://doi.org/10.1038/s41591-020-0820...lightning speed to elucidate as much as they could about this novel virus. SARS-CoV-2 entry ...

  10. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...Callahan KP et al. CBE Life Sci Educ. 2022 Dec;21(4):ar74. PMID: 36206327 (Link opens in a new window)...integrating MDH-related research into their teaching laboratories. They encourage instructors teaching different...research community and facilitates scientific collaborations between institutions. With these plasmids, ...regulation and post-translational modifications, and metabolic pathways and protein interactions. Plasmids A ...projects, find teaching resources and protocols, learn about regional hubs, access a teaching mentor, and more....2021.43 (Link opens in a new window) External Collaboration Results in Student Learning Gains and Positive...

  11. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Species Isotype 114473 Anti-TRIP8b (exon 4) [N212/3R] TRIP8b (exon 4) Rat Mouse IgG2a 114474 Anti-Clcn4 [N222...Human Mouse IgG2a 140081 Anti-Neuroligin-4* [N98/7R] Neuroligin-4* Mouse Mouse IgG2a 149451 Anti-GluK5/Grik5...Rat Mouse IgG2a 177522 Anti-TARPGamma2/4/8 [N245/36R] TARPGamma2/4/8 Rat Mouse IgG2a 177523 Anti-Cav1.2 ...Rat Mouse IgG2a 188168 Anti-Neuroligin-4* [N98/47R] Neuroligin-4* Mouse Mouse IgG2a 188169 Anti-Neuroligin...Human Mouse IgG2a 220426 TARPGamma2/4/8 [N245/36R-2b] TARPGamma2/4/8 Rat Mouse IgG2a 220427 Kir2.3 K+ ...231843 TARPGamma2/4/8 (Rattus norvegicus) recombinant mouse monoclonal antibody TARPGamma2/4/8 Rat Chimera...Human Mouse 211607 TRIP8b (exon 4) scFv [N212/3] N212/3 scFv TRIP8b (exon 4) Rat Mouse 211608 Synaptotagmin...

  12. mTOR Pathway

    Type
    Collection
    ..., Topisirovic I. Nat Rev Drug Discov. 2015 Apr;14(4):261-78. doi: 10.1038/nrd4505. PubMed PMID: 25743081... the cell and regulates the switch from anabolic to catabolic processes. Active mTORC1 promotes protein...mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates with poor prognosis in many...ammalian t arget o f r apamycin (mTOR) is a key metabolic regulator controlling cell growth and proliferation...energy-intensive processes. mTORC1 also blocks catabolic processes including autophagy and lysosome biogenesis...

  13. Chemogenetics AAV Preps

    Type
    Collection
    ... Fishell 83897 pAAV-hDlx-GqDREADD-dTomato-Fishell-4 hM3D(Gq) - Activation NLS-dTomato none 1, 9, rg* Gordon... Chemogenetics Guide pages for more information about this field. Receptor Activating hM3D(Gq) DREADD ...

  14. Lentiviral Prep Service

    Type
    Collection
    ...Resistance Purpose PI 17446 pLenti CMV GFP Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression...are reported with each shipment. For more details about our viral vector packaging service, visit our Viral...

  15. Brain Initiative Collection

    Type
    Collection
    ...Fishell 83897-AAV1 pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Gq-DREADD (P2A) nuclear dTomato expression in forebrain...Fishell 83897-AAV9 pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Gq-DREADD (P2A) nuclear dTomato expression in forebrain...Fishell 83897-AAVrg pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Gq-DREADD (P2A) nuclear dTomato expression in forebrain...particles that are suitable for in vivo use. Learn more about our viral service ID Viral Prep Description PI 65417...well as by our trusted partner labs. Learn more about our antibodies ID Recombinant Antibody Reactivity...

  16. TALEN Guide

    Type
    Collection
    ...Move over ZFNs. Nat Biotechnol. 2011 Aug 5;29(8):681-4. Pubmed PMID: 21822235 . See the Q&A with Dr. Adam...researchers who are learning to wield them. Learn more about Addgene’s TAL effector and TALEN kits here . References...

  18. Fluorescent Proteins: FRET

    Type
    Collection
    ...acceptor excitation spectra (×10 15 M -1 cm -1 nm 4 ). Values obtained from FPbase.org FRET calculator...intramolecular or unimolecular FRET). Learn more about FRET on our blog and explore the tables below for...fluorophores via HaloTag or SNAP-Tag . Read more about self-labeling tags on our blog . Luciferase can ...

  19. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...cycling 4-base Bsal-HFv2 37-16 °C cycling 4-base BsmBI-v2 42-16 °C cycling 4-base ... 1 hour 3-base or 4-base T4 DNA ligase 25 °C 18 hours 3-base or 4-base T4 DNA ligase... 1 hour 3-base or 4-base T4 DNA ligase 37 °C 18 hours 3-base or 4-base Bbsl-HF®, 1x ...Ligase MM 37-16 °C cycling 4-base Bsal-HFv2® 37 °C Static 4-base Bsal-HFv2, 1x NEBridge...-16 °C cycling 4-base PaqCl, 1x NEBridge Ligase MM 37-16 °C cycling 4-base Sapl              ...overhangs with the same three nucleotides in a row; (4) no more than two nucleotides in the same position...used. Each of the tools works with either 3-base or 4-base overhangs, and with a variety of different enzymes...

  20. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...scientific authorship in the United States (0.428). 4. The #1 (re)tweeted domain by scientists was Instagram...Sciencemag, Wiley, Cell and Scientific American Figure 4: Top Scientific Domains Tweeted by Biologists. 7. ...and analysis of scientists on Twitter. Plos One,12(4). doi:10.1371/journal.pone.0175368. PubMed PMID: 28399145...use Twitter professionally to network, learn more about alternative careers for scientists, and share cool....” This paper addresses the following questions about scientists on Twitter: who are they? What do they...
Showing: 1 - 20 of 407 results