... N/A (AAV) 4 286 Mouse Validation (mVAL) CRISPR Library 159391 Knockout Mouse Moffat 3rd 4 182 Mycobacterium...Bison sgRNA Library 169942 Knockout Human Ebert 3rd 4 2,852 Bonifacino Lab Human ubiquitination-related ...Knockout Libraries 213927, 213928 Knockout Cow Tan 2nd 4 on average 94,000 Bradley Human CRISPR Poison Exon...73178 (2 plasmid) Knockout Human Doench and Root 3rd 4 76,441 Broad GPP genome-wide Brie 73632 (1 plasmid...73633 (2 plasmid) Knockout Mouse Doench and Root 3rd 4 78,637 Broad GPP Humagne Set C and Humagne Set D 172650... 209551 209552 Knockout Human Doench 3rd 1, 2, or 4 49,766 arrays Broad GPP kinome Brunello 75314, 75315...75313 (2 plasmid) Knockout Human Doench and Root 3rd 4 3,052 Broad GPP kinome Brie 75317 (1 plasmid) 75316...

...Type Plasmid Name Bait Cd4d3+4-bio Prey Cd4d3+4-COMP-b-lac His-tag Cd4d3+4-6his Bio-linker-his Cd4d3-bio-linker... FLAG prey CD200-Cd4d3+4-COMP-blac-3xFLAG-6his Cd4d3+4-pentamer BasiginCd4d3+4-COMP-his-stop Type II expression...Bateman A and Wright GJ. Genome research 2008 Apr; 18(4):622-30. A benchmarked protein microarray-based platform...important in cellular recognition processes. Laboratories interested in these approaches have created ... and secreted proteins Mouse sperm Mouse egg Laboratories using AVEXIS: Christian Sollner, Max Planck ...

...heavy diversity 4-23 (non-functional) IGHD423 IGHD4-4 immunoglobulin heavy diversity 4-4 DA4, IGHD44 IGHD5...heavy variable 4-30-2 IGHV4-3, IGHV4302 IGHV4-30-4 immunoglobulin heavy variable 4-30-4 IGHV4-3, IGHV4304...immunoglobulin heavy variable 4-39 IGHV439, VH IGHV4-4 immunoglobulin heavy variable 4-4 IGHV44, VH IGHV4-59 immunoglobulin...104A BD-4, DEFB-4, DEFB104, DEFB4, MGC118942, MGC118944, MGC118945, hBD-4 DEFB4 defensin, beta 4 DEFB-2...lambda variable 4-3 IGLV43, V5-1 IGLV4-60 immunoglobulin lambda variable 4-60 IGLV460, V5-4 IGLV4-69 immunoglobulin...hIL-3Ra IL4 interleukin 4 BCGF-1, BCGF1, BSF1, IL-4, MGC79402 IL4R interleukin 4 receptor CD124, IL4RA ...

...NR2A1, NR2A21, TCF, TCF14 hepatocyte nuclear factor 4, alpha NR0B1 Plasmids NR0B1, AHC, AHCH, AHX, DAX-1...NGFIB, NP10, NUR77, TR3 nuclear receptor subfamily 4, group A, member 1 NR4A2 Plasmids NR4A2, HZF-3, NOT...NOT, NURR1, RNR1, TINUR nuclear receptor subfamily 4, group A, member 2 NR5A1 Plasmids NR5A1, RP11-101K10.1...nuclear receptor plasmid collection assembled in collaboration with the Nuclear Receptor Signaling Atlas (NURSA...associated with numerous disease pathways. In collaboration with research labs from around the globe, Addgene...

...2023 May 4. Kaspar Podgorski Glutamate FRET sensor to monitor glutamate transport and metabolism (FLIPE)...tetramethylrhodamine-labeled ParM. ACS Chem Biol. 2010 Apr 16;5(4):415-25. Martin Webb ATP Multicolor palette of ATP...Dynamics. Cell. 2020 Nov 17. pii: S0092-8674(20)31399-4. Edward Boyden cAMP (cyclic AMP) FRET sensors of cAMP... and with high affinity. PLoS One. 2015 Apr 14;10(4):e0122513. Kees Jalink cAMP (cyclic AMP) Fluorescent... chloride in neuronal processes. PLoS One. 2012;7(4):e35373. Susan Amara , Susan Ingram Citrate Nanosensors...Single beta Cells. Cell Chem Biol. 2017 Apr 20;24(4):525-531.e4. Dmytro Yushchenko Lactate Laconic FRET-based... Fluorescent Biosensor. Cell Metab. 2011 Oct 5;14(4):545-54. Gary Yellen NADH/NAD+ Peredox fluorescent...

...small multiplex experiments 4-color flow panel: BV421, FITC, PE, and APC 4-color microscopy panel: DAPI...protects mucosal membranes Dimer with a valency of 4 IgA1; IgA2 IgD Coexpressed with IgM on the surface... As a guideline, most antibodies can be stored at 4 °C for short-term storage. For long-term storage they...conjugated to fluorophores should never be stored below 4 °C. Certain isotype subclasses, such as IgG3, aggregate...aggregate after thawing and therefore should be kept at 4 °C. Record and track lot numbers for all antibodies...typically use HRP as a signaling molecule. Figure 4: A direct sandwich ELISA, which uses a capture antibody...are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments, each recognizing...

...reverse complement oligo ( e.g., sgRNA-A in Table 4 ). The resultant oligos would be 25-mer oligos. However... G, do not add another G ( e.g., sgRNA-B in Table 4 ) and do not add C to the final position of the reverse...the resultant oligos would be 24-mer oligos ( Table 4 ). Design Deletion Screening Primers Design one set...delivery of CRISPR/Cas9 plasmids by electroporation 4 . This protocol is described in detail for murine ...article from Journal of Visualized Experiments (JoVE) about genomic deletions in mammalian cell lines using ...up to 20 μl. Use the primers designed in step 2 above. Conduct PCR for “non-deletion band” and “deletion...

...protein and is involved in virus entry into cells. ( 4 ) Cathepsin L - a cysteine endosomal protease that...viral infectivity.( 6 ) PCP4 - (Purkinje cell protein 4) is involved in beating of airway cilia.( 7 ) Cytokines...and Influenza. Abbott, et al. Cell. 2020 May 14;181(4):865-876.e12. (Link opens in a new window) CRISPR ...lightning speed to elucidate as much as they can about this novel virus. It is now known that SARS-CoV-...and open-access SARS-CoV-2 detection assay for laboratory and home testing. Kellner, et al. bioRxiv 2020.06.23.166397...

...Titer Typical Titer Small 10 × 100 µL aliquots 1 mL 4 × 10 12 GC/mL* 1 × 10 13 GC/mL* Medium 25 × 100 µL...scientist through an MTA. This process typically takes 1–4 days, depending on your institution. AAV Production...Additional Resources Viral Vector Production Learn about how we produce high-quality viral vector preps at...

...double-strand break (DSB) within the target DNA, located ∼3–4 nucleotides upstream of the PAM sequence. The resulting...Modifications Using Homology Directed Repair (HDR) Figure 4: Overview of HDR HDR generates specific genomic edits... for In Vivo Genome Editing. Molecular Cell , 73 (4), 714-726.e4. PMID: 30581144 Fu, Y., Foden, J. A.,...engineered CjCas9 orthologs. Molecular Therapy , 31 (4), 1177–1187. PMID: 36733251 Harrington, L. B., Doxzen...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman,...CRISPR/CAS-Mediated Genome Engineering. Cell , 153 (4), 910–918. PMID: 23643243 Zhang, Y., & Marchisio, ...cytidine deaminase fusions. Nature Biotechnology . 35 (4), 371–376. PMID: 28191901 Komor, A. C., Kim, Y. B....

... Cas9 in astrocytes. Endo et al Science. 2022 Nov 4. Base edit human mitochondrial DNA using mitoBEs ....transmission. Aggarwal et al. Nat Methods. 2023 May 4. See More iPSC Differentiation Factors Find plasmids...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...Scientists are studying protein aggregation, learning about the blood brain barrier integrity, and using CRISPR...with support from the BRAIN Initiative. Jackson Laboratory (Link opens in a new window) A collection of ...

... miRFP703 673/703 8 pmiRFP703-N1 miRFP709 683/709 4 pmiRFP709-N1 iRFP (aka iRFP713) 690/713 6.2 piRFP ...Proteins for in vivo imaging have emission near or above 650 nm as signals below 650 nm are highly absorbed... interactions in vitro and in vivo . Learn more about in vivo imaging tools in this blog post - In Living...

...Callahan KP et al. CBE Life Sci Educ. 2022 Dec;21(4):ar74. PMID: 36206327 (Link opens in a new window)...integrating MDH-related research into their teaching laboratories. They encourage instructors teaching different...research community and facilitates scientific collaborations between institutions. With these plasmids, ...regulation and post-translational modifications, and metabolic pathways and protein interactions. Plasmids A ...projects, find teaching resources and protocols, learn about regional hubs, access a teaching mentor, and more....2021.43 (Link opens in a new window) External Collaboration Results in Student Learning Gains and Positive...

...Request service, Addgene guarantees a titer of at least 4 x 10 12 GC/mL for the AAV1, AAV5, AAV8, or AAV Retrograde... by email will supersede the general guarantees above. We will have our expert viral vector production...supersede the general estimated turnaround time above. After starting viral production, if we determine...

...iSPI_pSer_Subpool#3 Pooled Library 188529 iSPI_pSer_Subpool#4 Pooled Library 188530 iSPI_pSer_Subpool#5 Pooled Library...Rinehart J, Söll D. Science . 2011 Aug 26;333(6046):1151-4. PubMed (Link opens in a new window)...This library is anticipated to be useful for laboratories performing phosphoproteomic experiments in need...

...based on the Cas9 D10A nickase described in Figure 4 of the Cong, et. al, 2013 Science paper. For example... Fragment Length Polymorphism (RFLP) ( see Figure 4 of the Cong, et. al, 2013 Science paper). If you detect... Feng Zhang's Lab, has assembled a list of FAQs about using the lab's CRISPR technology. Genome..., February 2013 ), has assembled a list of FAQs about using the lab's CRISPR technology. Visit the Zhang...

...Species Isotype 114473 Anti-TRIP8b (exon 4) [N212/3R] TRIP8b (exon 4) Rat Mouse IgG2a 114474 Anti-Clcn4 [N222...Human Mouse IgG2a 140081 Anti-Neuroligin-4* [N98/7R] Neuroligin-4* Mouse Mouse IgG2a 149451 Anti-GluK5/Grik5...Rat Mouse IgG2a 177522 Anti-TARPGamma2/4/8 [N245/36R] TARPGamma2/4/8 Rat Mouse IgG2a 177523 Anti-Cav1.2 ...Rat Mouse IgG2a 188168 Anti-Neuroligin-4* [N98/47R] Neuroligin-4* Mouse Mouse IgG2a 188169 Anti-Neuroligin...Human Mouse IgG2a 220426 TARPGamma2/4/8 [N245/36R-2b] TARPGamma2/4/8 Rat Mouse IgG2a 220427 Kir2.3 K+ ...231843 TARPGamma2/4/8 (Rattus norvegicus) recombinant mouse monoclonal antibody TARPGamma2/4/8 Rat Chimera...Human Mouse 211607 TRIP8b (exon 4) scFv [N212/3] N212/3 scFv TRIP8b (exon 4) Rat Mouse 211608 Synaptotagmin...

..., Topisirovic I. Nat Rev Drug Discov. 2015 Apr;14(4):261-78. doi: 10.1038/nrd4505. PubMed PMID: 25743081... the cell and regulates the switch from anabolic to catabolic processes. Active mTORC1 promotes protein...mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates with poor prognosis in many...ammalian t arget o f r apamycin (mTOR) is a key metabolic regulator controlling cell growth and proliferation...energy-intensive processes. mTORC1 also blocks catabolic processes including autophagy and lysosome biogenesis...

...and Resources Missing in the Field Kenton Woodard 4:00 PM Wrap Up & Next Steps Melina Fan/Leila Haery ...Learn about Addgene's 2018 AAV Education and Development (AAVED) workshop series. The... Institute Katherine Matho Cold Spring Harbor Laboratory Gowan Tervo Janelia Research Campus Sarada Viswanathan...Institute Katherine Matho, Cold Spring Harbor Laboratory Gowan Tervo and Sarada Viswanathan, Janelia Research...

... Fishell 83897 pAAV-hDlx-GqDREADD-dTomato-Fishell-4 hM3D(Gq) - Activation NLS-dTomato none 1, 9, rg* Fishell... Chemogenetics Guide pages for more information about this field. Receptor hM3D(Gq) DREADD rM3D(Gs) DREADD...