We narrowed to 529 results for: arl
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  TypeBlog PostPublishedMarch 28, 2018, 6:52 p.m....xCas9 3.7. Base editor variants with xCas9 are similarly flexible. Like the xCas9 3.7 nuclease, xCas9 3.7... and base editing, to more loci. Although these early data are exciting, there’s a lot we still don’t ...
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  Antibodies 101: Conventional vs Spectral Flow CytometryTypeBlog PostPublishedAug. 3, 2023, 1:15 p.m....cytometry (“spectral flow”) has emerged as a particularly useful tool for those wishing to run larger ...Mayo Clinic studying host-microbe interactions in early life. Resources More resources on the Addgene...
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  Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPRTypeBlog PostPublishedJuly 2, 2020, 12:40 p.m....creates chimeric circles of DNA that are distant linearly but close together in space. The chromatin “picture...enhancer is interacting with. Super enhancers are particularly good models for building chromatin interaction...
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  Academic vs. Industry PostdocsTypeBlog PostPublishedNov. 13, 2018, 1:22 p.m....experience, the more I heard that getting into industry early would only help. Like any major life decision, ...could be laid off while you’re there. Of course, similarly, an academic advisor could also lose their funding...
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  Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary CellsTypeBlog PostPublishedDec. 10, 2020, 1 p.m....expression cassette in one single viral particle. The earliest explorations of the use of AdV for in vivo CRISPR-Cas...of AdV-modified human cells being subjected to scarless homology-directed genome editing (Maggio et al...
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  10 Basic tips for mammalian cell cultureTypeBlog PostPublishedJuly 12, 2018, 1:09 p.m.... close to the hood so you can view your cells regularly (both before entering the hood and when putting...Institute of Colloids and Interfaces. Sana is particularly interested in Glycobiology and Immunology. You...
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  PCR Applications for Copy Number Variation AssaysTypeBlog PostPublishedApril 26, 2022, 1:15 p.m....dsDNA in most starting materials, and is not particularly sensitive. The fluorescent signal can be affected...be affected by DNA quality and primer design, particularly for amplicons under 100 bp (Ma, 2015.) A schematic...
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  Light Sheet Fluorescence MicroscopyTypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....seen some major advances in light microscopy, particularly related to the neurosciences. These developments...tetrahydrofuran-based tissue clearing (13,14). A few years later, Karl Deisseroth’s lab developed CLARITY, which is a hydrogel-based...
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  Which Fluorescent Protein Should I Use?TypeBlog PostPublishedMay 20, 2014, 2:06 p.m....The maturation of the chromophore on many FPs (particularly those derived from GFP) requires oxygen. Therefore...interested in everything related to gene expression, particularly at the RNA level. He maintains the greenfluorescentblog.wordpress.com...
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  A Guide to Starting Your Own Journal ClubTypeBlog PostPublishedFeb. 6, 2020, 3 p.m....didn’t attend. For example, we got a good bit of early feedback from those who wanted, but were unable ...carefully organized. Most importantly, they should be regularly assessed for continued relevance and if needed...
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  SciComm: Taking Science to Elementary and Middle SchoolsTypeBlog PostPublishedJuly 9, 2019, 12:52 p.m....experiences. STEM in elementary schools - catching them early I’m fortunate to live in a town with a good public...means it was the best kind of networking! Since nearly every talk I’ve given in the past 20 years has ...
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  New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa LibrariesTypeBlog PostPublishedOct. 4, 2018, 12:44 p.m....information while using less resources and are particularly useful when trying to screen a limited number...discriminate between non-essential and essential genes similarly to the Brunello knock-out library while mitigating...
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  Better Dyeing Through Chemistry & Small Molecule FluorophoresTypeBlog PostPublishedSept. 8, 2016, 2:30 p.m....conjugate, and the complexity of the system. A particularly desirable property for small-molecule labeling...removing excess dye from the sample, which is particularly important for samples where washing is difficult...
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  CRISPR Between the Genes: How to Experiment with Enhancers and EpigenomicsTypeBlog PostPublishedAug. 9, 2016, 2:30 p.m....mere millimeters apart. Similarly, cells compact DNA in a way that brings linearly distant regions close...
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  Lentiviral Vector Uses and OverviewTypeBlog PostPublishedMay 19, 2016, 2:30 p.m....Lentiviral vector history and components In the early 1990s, researchers developed viral vector systems... but this research is still in the pre-clinical/early clinical stages. Researchers are also working to...
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  Twenty Years of Sharing: Expanding AccessibilityTypeBlog PostPublishedNov. 21, 2024, 10:47 p.m....easier to find materials and learn about them. As early as 2007, we began grouping similar tools together...12 guides available on our website, which are regularly used by scientists around the world. Collectively...
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  Hot Plasmids Spring 2024TypeBlog PostPublishedMay 23, 2024, 1:15 p.m.... Janelia, are now available! Building on their earlier Voltron indicators (a fusion of Ace2N and HaloTag...Neurodegeneration Research Collection. This page is regularly updated with useful materials for research into...
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  pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCsTypeBlog PostPublishedDec. 14, 2017, 2:08 p.m....reprogramming from 0.001% to nearly 0.1% [7]. In terms of hiPSC colonies, nearly 16 hiPSC colonies can now...
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  Career Insights: Technical Support SpecialistTypeBlog PostPublishedNov. 9, 2017, 2 p.m.... your technical knowledge and judgement. For particularly tricky cases, team discussions can often lead...doing a lot of science communication, and is particularly excited to hear about novel developments in ...
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  Four Base Editing Reporters to Monitor and Enrich Editing in Real-timeTypeBlog PostPublishedJuly 7, 2020, 1:15 p.m.... Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin resistance...translation of an array of different reporters including mScarlet-I, luciferase, antibiotic resistance markers, ...