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We narrowed to 520 results for: arl

Showing: 201 - 220 of 520 results

  1. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...dsDNA in most starting materials, and is not particularly sensitive. The fluorescent signal can be affected...be affected by DNA quality and primer design, particularly for amplicons under 100 bp (Ma, 2015.) A schematic...

  2. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...cytometry (“spectral flow”) has emerged as a particularly useful tool for those wishing to run larger ...Mayo Clinic studying host-microbe interactions in early life.    Resources More resources on the Addgene...

  3. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...may be too constraining for some experiments, particularly in vivo ones. In those cases, AAV vector screens...your CRISPR screen to a high degree. This is particularly helpful when investigating tissue-specific diseases...

  4. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...experience, the more I heard that getting into industry early would only help. Like any major life decision, ...could be laid off while you’re there. Of course, similarly, an academic advisor could also lose their funding...

  5. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...seen some major advances in light microscopy, particularly related to the neurosciences.  These developments...tetrahydrofuran-based tissue clearing (13,14). A few years later, Karl Deisseroth’s lab developed CLARITY, which is a hydrogel-based...

  6. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...The maturation of the chromophore on many FPs (particularly those derived from GFP) requires oxygen. Therefore...interested in everything related to gene expression, particularly at the RNA level. He maintains the greenfluorescentblog.wordpress.com...

  7. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...experiences. STEM in elementary schools - catching them early I’m fortunate to live in a town with a good public...means it was the best kind of networking! Since nearly every talk I’ve given in the past 20 years has ...

  8. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...didn’t attend. For example, we got a good bit of early feedback from those who wanted, but were unable ...carefully organized. Most importantly, they should be regularly assessed for continued relevance and if needed...

  9. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...information while using less resources and are particularly useful when trying to screen a limited number...discriminate between non-essential and essential genes similarly to the Brunello knock-out library while mitigating...

  10. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...conjugate, and the complexity of the system. A particularly desirable property for small-molecule labeling...removing excess dye from the sample, which is particularly important for samples where washing is difficult...

  12. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...Lentiviral vector history and components In the early 1990s, researchers developed viral vector systems... but this research is still in the pre-clinical/early clinical stages. Researchers are also working to...

  15. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...What is an aptamer? Nearly 30 years ago, two independent groups, led by Jack Szostak and Larry Gold, ... Assays that require recognition of ions or particularly small molecules that do not generate an immune...

  17. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...CRISPR Cas variant that can also cleave dsDNA similarly to Cas9 (Zetsche et al., 2015 ). Cas12a however...DETECTR. How does DETECTR work? DETECTR works similarly to SHERLOCK. Cas12a is targeted to a specific ...

  18. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ... to keep up with the latest tools. In Addgene’s early days, we would ‘spot-check’ critical regions of ...us to keep pace with our current need to analyze nearly 300 sequences each week. Our internal software ...

  19. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...easier to find materials and learn about them. As early as 2007, we began grouping similar tools together...12 guides available on our website, which are regularly used by scientists around the world. Collectively...

  20. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ... Janelia, are now available! Building on their earlier Voltron indicators (a fusion of Ace2N and HaloTag...Neurodegeneration Research Collection. This page is regularly updated with useful materials for research into...
Showing: 201 - 220 of 520 results