We narrowed to 525 results for: ato

...for students, postdocs, and young Principal Investigators (PIs). We've interviewed Addgene depositors ...different ways to succeed – as loner or gregarious;  dictator or laissez-faire; theory or practice; business-like...

...through Caitlin’s blood. “I come from a family of educators, mostly elementary school, middle school, and ...school, I actually served as the student course coordinator. With the guidance of one of the faculty members...

...For this series, I’ll interview three science communicators who work in the biotech, education, or nonprofit...job. “I am really more of a multimedia science communicator than a science writer these days. I do some ...

...metadata for each screen is captured by BioGRID curators, including the cell line/tissue type, enzyme system...CRISPR database are encouraged to contact BioGRID curators at [email protected] to submit their published...

...Esteban Engel New serotype pAAV-hSyn-Cre-P2A-dTomato AAV PHP.eB Recombinases Rylan Larsen New serotype...multiple serotypes pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] AAV1 Optogenetics Edward Boyden New serotype ...

...used to target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA...itself, simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer ...

...applications. Also, purifying GST by affinity chromatography requires proper folding of the tag, which prevents...to be purified by single step metal affinity chromatography. Purification of His fusion proteins from E...

...line is known to be genetically manipulated, its anatomical origin, if cell line has ever been the target...informative. Spoiler alert: HeLa is the most common contaminator line.  For those wanting to access Cellosaurus...

...of DNA that can move around in a genome. In a laboratory setting, transposons can be used to both introduce...use of viruses is also important for social and regulatory reasons. 1.) The transposon consists of a mirrored...

... able to eliminate the need for axenic (pure) laboratory cultures to study these microbes. From 16S rRNA...between the battery bank and the vehicle battery/alternator to make sure the vehicle battery didn't run down...

...Washington University in St. Louis provides a combinatorial indexing strategy that simultaneously tracks...2019). Key takeaways of CellTag CellTag is a combinatory cell-indexing tool that combines scRNA-seq and...

... more than 100,000 samples to researchers and educators in 60 countries each year. Introducing Arabidopsis...Science and Industry (COSI) and the Franklin Park Conservatory. Do you use Arabidopsis in your research? Are...

...them. CRISPR Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening CombiGEM is a technology that...Albert Cheng and Mark Wanner from the Jackson Laboratory for Genomic Medicine described Casilio, a system...

...delivery of Cas9 endonuclease, Cas9 nickase, Cas9 activators, Cas9 repressors, select gRNAs, and CRISPR pooled...expression of a gene under the control of the tet operator. AAV AAVs are the workhorses of both chemo- and...

...skills include: Science Reporter Scientific Illustrator Research and Development Scientific Advisor..., including: Livestock Breeder Scientific Illustrator Plant Breeder Zoological Worker Conservationist...

...relevant science."     - Randall Carter, Contracts Administrator Inferior: How Science Got Women Wrong - and ... Tsang, Science Communications and Marketing Coordinator Non-science favorites  The Mistborn series by...

...37825  AAV1 pAAV-CAG-GFP 59462  AAV2 pAAV-CAG-tdTomato (codon diversified) 114472  AAV5, AAV8 pAAV-...ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid Serotype Name 99120  AAV1 pAAV-ihSyn1...

...serotonin is a well-known but poorly studied neuromodulator, and evidence suggests that it is incredibly...sensor). In preliminary studies with the lab’s collaborators, they were able to use the serotonin sensor ...

... at 230 nm. The A260/A280 ratio is used as an indicator of DNA purity. Ideally, this number should be ...like a PCR product). This method also gives an indicator of DNA or RNA contamination based on the presence...

...For my undergraduate degree, I majored in human anatomy, but I also took classes in biochemistry, microbiology...the time I was ready to graduate with my BSc in Anatomy, the University of Otago had established the Centre...