We narrowed to 462 results for: bli
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TypeBlog PostPublishedDec. 19, 2024, 2:15 p.m....was frustrated by the difficulty of accessing published plasmids from other scientists. She, Kenneth Fan...others could request from — Addgene! Addgene was established as an e-commerce company, which helped it stay...
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Antibodies 101: Conjugation
TypeBlog PostPublishedMarch 26, 2024, 1:15 p.m....BioRender.com. Many biologists can lead perfectly blissful, productive lives without thinking about these...10.1016/j.it.2012.02.010 Dennler P, Fischer E, Schibli R (2015) Antibody Conjugates: From Heterogeneous... -
RANbodies: Reporter Nanobody Fusions
TypeBlog PostPublishedApril 10, 2018, 12:56 p.m....tissues. Although antibody production is well established, it’s also a process that’s difficult for individual...their RANbodies, Yamagata et al used previously published nanobody sequences that targeted the following... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog PostPublishedSept. 22, 2015, 2:30 p.m.... research environment” with a strong focus on publishing, but still made it so she could “get exposure...summarized in a recent Nature Medicine paper. In the publication, Bhang and coworkers used the ClonTracer library... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog PostPublishedApril 22, 2015, 2:06 p.m....they’ve gone along, Voigt’s group has also been assembling the toolbox needed for anyone to begin considering... a very big way. In one of his latest papers, published in Molecular Systems Biology in November, Voigt... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m....bacteria can also have serious implications for public health. Bacteriocin plasmids: microbial offense...toxic substances like heavy metals or pesticides, enabling their hosts to survive where others can’t. This... -
Making CRISPR Plasmids Using Fragmid
TypeBlog PostPublishedAug. 29, 2024, 1:15 p.m....the Fragmid system by sequencing 60 individual assemblies created over five months. When they tested those...were perfectly correct. Because they created the assemblies in duplicate, they were able to confirm that ... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog PostPublishedJuly 23, 2020, 1:20 p.m....Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology... E, Bondy-Denomy J, Corn JE, Doudna JA (2017) Disabling Cas9 by an anti-CRISPR DNA mimic. Sci Adv 3:e1701620... -
Antibodies 101: Choosing the Right Isotype
TypeBlog PostPublishedApril 6, 2023, 3 p.m....Cytochemistry (Vol. 46, Issue 8, pp. 977–983). SAGE Publications. https://doi.org/10.1177/002215549804600813 ...Sturtevant (Ed.), PLoS ONE (Vol. 7, Issue 6, p. e38313). Public Library of Science (PLoS). https://doi.org/10.1371... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog PostPublishedJuly 12, 2021, 1:15 p.m....Originally published Apr 30, 2020 and last updated Jul 12, 2021. What started out as a small collection...lab instead of a BSL-3 lab. Jesse Bloom’s lab published a protocol and reagents for pseudotyping lentivirus... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog PostPublishedMay 4, 2021, 1:15 p.m....tested for antigen binding. If starting from an established hybridoma line, the antibody can be converted...Edition. 2104. Cold Spring Harbor Laboratory Publications, New York. Helma J, Cardoso MC, Muyldermans ... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog PostPublishedMarch 8, 2017, 3:15 p.m....name and match the name as it appears in its publication or as it is commonly known in your lab. Plasmid...Distribution status for your plasmids: Hold for Publication or Distribute pending QC. The name of the Principal... -
Plasmids 101: Modular Cloning
TypeBlog PostPublishedMarch 12, 2024, 1:15 p.m...., a pre-made MoClo kit, is a prime choice for assembling an array of targeting constructs and delivering... many different MoClo kits have already been established. Keep an eye out for our next blog on choosing... -
Hot Plasmids February 2024
TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m....original, though its maturation is even faster. Published just a few days later, Mohan Balasubramanian and...antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing. In a proof-of-concept study, mouse... -
CRISPR in the Clinic
TypeBlog PostPublishedNov. 2, 2023, 1:15 p.m....substantial relief from both disorders. As of publication, submissions to the UK, EU, and USA federal drug...cardiovascular disease, inflammatory diseases, blindness, and diabetes. Directions and obstacles for future... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog PostPublishedDec. 1, 2015, 3:30 p.m....they are making fairly remarkable progress on establishing a system for the chemical replication of RNA...created a circRNA website so scientists can view public circRNA datasets and download the custom python... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog PostPublishedJuly 23, 2015, 2:30 p.m....recently there has been a counter movement by the public to access basic equipment and to become involved...first hackerspace which now offers members and the public cheap access to personal manufacturing tools – ... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog PostPublishedOct. 22, 2014, 1:57 p.m....This post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates...these people and bios were likely part of the publicity. Good panels do not waste time on long introductions... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog PostPublishedAug. 29, 2019, 12:50 p.m....poster board well ahead of time ensures you aren’t scrambling to tack up your poster seconds before the session...about the relationship of your work to recently published papers or “hot topics,” so be sure to be well-... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog PostPublishedApril 4, 2019, 12:53 p.m....constant, with fitness effects more pronounced at 12 doublings than at 6. Key takeaways of Mobile-CRISPRi Mobile-CRISPRi...PubMed PMID: 30675034. Peters, Jason M., et al. "Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi...