We narrowed to 319 results for: bre

1. Targeted Mutagenesis with EvolvR

   Type

   Blog Post

   Published

   Feb. 21, 2019, 1:15 p.m.

   ...tracing cells that do not tolerate double-strand DNA breaks by introducing a unique tag into a genomic site...

2. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

   Type

   Blog Post

   Published

   Feb. 19, 2019, 1:36 p.m.

   ... it available to everyone, that creates more breakthrough and innovation. With any kind of open platform...

3. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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   Blog Post

   Published

   Sept. 15, 2020, 1:15 p.m.

   ...These cells, presumably the cell you want, cannot break down X-gal and are white. Cells that don’t contain...

4. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

   Type

   Blog Post

   Published

   May 26, 2020, 1:15 p.m.

   ...be targeted by a sgRNA guided Cas9 causing a DSB break. This DSB is repaired by NHEJ which depending on...

5. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

   Type

   Blog Post

   Published

   March 17, 2020, 1:32 p.m.

   ...the DNA base change without a DNA double-strand break. But base editors aren’t perfect. They may be slow...

6. Building and Validating Recombinant Antibodies: A Case Study

   Type

   Blog Post

   Published

   April 5, 2022, 1:20 p.m.

   ... Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. A toolbox of IgG subclass-switched...

7. Sweating the Small Stuff: Details in the Lab

   Type

   Blog Post

   Published

   Feb. 8, 2022, 2:15 p.m.

   ...intentionally slow down, you’re giving yourself a chance to break what can be a rather vicious cycle.  Everybody’s...

8. Viral Vectors 101: Viral Vector Elements

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   Blog Post

   Published

   July 25, 2023, 1 p.m.

   ...components are called helper plasmids. Below, we’ll breakdown the vector components first by virus and then ...

9. Viral Vectors 101: Types of viruses

   Type

   Blog Post

   Published

   June 6, 2023, 1:15 p.m.

   ...through nuclear pores and thus do not require breakdown of the nuclear envelope during cell division to...

10. Viral Vectors 101: The AAV Retrograde Serotype

    Type

    Blog Post

    Published

    April 27, 2023, 1:15 p.m.

    ...transduced at all by this serotype. Nichole Beebe in Brett Schofield’s lab demonstrated that pathways projecting...

11. Viral Vectors 101: Viral Applications

    Type

    Blog Post

    Published

    April 13, 2023, 1:15 p.m.

    ...Zaunders JJ, Gabuzda D, Crowe SM, Mills J, Lewin SR, Brew BJ, Cunningham AL, Churchill MJ. Pathogenicity and...

12. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type

    Blog Post

    Published

    Aug. 6, 2024, 1:15 p.m.

    ...only able to enter the nucleus during mitotic breakdown of the nuclear envelope. Lentiviruses can transduce...

13. Viral Vectors 101: Viruses as Biological Tools

    Type

    Blog Post

    Published

    March 21, 2023, 1:15 p.m.

    ..., as their access to the host genome requires breakdown of the nuclear envelope, which occurs during mitosis...

14. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    Published

    July 28, 2015, 2:30 p.m.

    ...offers high efficiency of inducing double strand breaks and more opportunity for homologous recombination...

15. Plasmids 101: Blue-white Screening

    Type

    Blog Post

    Published

    June 4, 2015, 1:03 p.m.

    ... When expressed, the β-galactosidase enzyme can break down a dye-linked substrate called x-gal (5-bromo...

16. How to Make Friends and Meet People at a Scientific Conference

    Type

    Blog Post

    Published

    Aug. 7, 2014, 1:58 p.m.

    ... you are interested in and this is a great ice breaker. Of course, real relationships go further and grow...

17. 3 Challenges in Plant Synthetic Biology

    Type

    Blog Post

    Published

    July 22, 2014, 5:46 p.m.

    ...the day cannot be developed through conventional breeding; synthetic biology is required to achieve this...

18. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type

    Blog Post

    Published

    July 10, 2014, 7:17 p.m.

    ...(A) Productize, (B) Roll out to market, and (C) Break even cycles. Work hard. Work fast. Be flexible. ...

19. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type

    Blog Post

    Published

    Feb. 18, 2014, 4:22 p.m.

    ...schedule might work. If not, perhaps a 3 hour breakfast once a month. All participants must commit to ...

20. dTAG - You're it!

    Type

    Blog Post

    Published

    June 21, 2018, 2:06 p.m.

    ...not necessary for the proliferation of basal-like breast cancer cells. eLife. September 2017. 6: e26693....