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Addgene

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Showing: 201 - 220 of 756 results

  1. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...working day, including appropriate MTAs and customs forms. Of course, ordering the plasmids is the easy part...

  2. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...ligase complex. Finally, when auxin is added in the form of auxin hormone indole-3-acetic acid (IAA), the...behave with a new protein, but you can make an informed choice! References and Resources References Bondeson...

  4. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...exogenously to measure a phenotypic response in the form of fluorescence or voltage indicators.   Viral... here!). The only issue with these GEVIs? They perform poorly in two-photon conditions, which are necessary...

  5. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...’t be lazy about splitting cells, instead try to form a routine. Twice a week often works for most fast-growing...

  6. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...transsynaptically). This is because the recombinant forms of these viruses typically used in research aren...after, we learned about organs and the jobs they perform. This became foundational later on when we studied...

  7. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...MMEJ (microhomology-mediated end-joining)? MMEJ, a form of alternative end-joining, requires only very small...’s become clear that the power of this editing platform lies in the diversity of nucleases and their applications...

  8. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ... in spacer acquisition following infection, as a form of “bait” to identify new CRISPR-associated proteins...target binding and cleavage through a series of conformational changes in the Cas13a molecule. Like Cas9, ...

  9. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...changes in gene expression as new body sections are formed. In synthetic biology, FP timers might be able ...isolate particular organelles and their contents, or perform in situ hybridization experiments. In many cases...like the patterning of nascent tissues and the formation of limbs are correlated with changes in gene expression...and developmental timer of neural fiber bundle formation." Journal of Biological Chemistry 276.32 (2001...

  10. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    .... For instance, the fluorophore and quencher may form the base of the stem in an RNA stem-loop structure...and detection - since they frequently undergo conformational changes after target binding, aptamers are ...fluorescence-activated cell sorting (FACS) of E. coli transformed with aptamer libraries, the Jaffrey group selected...

  11. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ... local family to help, create “fake” family by forming a social circle of people in the same situation... and/or your spouse won’t be at every school performance, chaperone every field trip or cheer at every...

  12. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...can also protect against proteolysis. That’s one form of instability. Prokaryotes can also have a hard...Thioredoxin A (TrxA) that improve disulfide bond formation in order to help keep your protein soluble.  ... better assess their function. More detailed information and some protocols can be found in the references...

  13. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...and others have reported that FP cysteine residues form inappropriate disulfide bonds in the secretory pathway...modifications including glycosylation, disulfide bond formation, and proteolytic cleavage. FPs localized to organelles...fusion protein localization. For example, the formation of inappropriate inter-chain disulfide bonds between...because of the correct folding and fluorophore formation by most, if not all, of the FP fusions. Our moxFPs...

  14. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...Barcoded tubes come in many forms. The 2D barcode can store a lot of information in a small space and is ... treated to easily take in plasmids (transformation). Transformed bacteria are then grown up on antibiotics...received is immediately transformed and stored. Some samples need to be transformed with a micropulser using... (98.2%) transform the first time and can be stored right away. Every week, the lab performs an average...average of 140 transformations. The lab typically transforms newly deposited samples from Monday through Wednesday...delivered by a mail courier and then the journey of transformation and storage begins. Some samples are submitted...barcoded tubes that are scanned into our Laboratory Information Management System (LIMS), a database we use to...

  15. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...choice, you should perform a functional assay to make sure your protein isn’t forming inclusion bodies ...

  16. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...mechanism (see next section for details). The newly formed single-stranded DNA (ssDNA) is then pulled through...through Cas3. After these initial steps, Cas3 can perform its helicase and nuclease functions simultaneously... D. W. (2023). Structural snapshots of R-loop formation by a type I-C CRISPR Cascade. Molecular Cell, ...

  17. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...expressed in E. coli, in mammalian cells, or in purified form, and didn’t need any added cofactors.      ...future MagLOV-based tools, just as light-induced conformational changes are the basis of many optogenetic tools...have collected a variety of data that might help formulate some hypotheses. They are excited to see other...

  18. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...secreted into the periplasmic space to be oxidized and form disulfide bonds (Frenzel et. al., 2013). If you’...applications. An antibody that performs well in immunohistochemistry may not perform well in a western blot. ... than mammalian cells, but mammalian cells can perform human-like post-translational modifications that... If that’s the case, a buffer exchange can be performed using a desalting column or an ultrafiltration...ones, consider increasing the number of washes performed during your harvest. Validation Recombinant antibodies.... This can be due to conformational changes in the antigen or lower detection sensitivity of the assay...S. Sarangapani, Protein aggregation, particle formation, characterization & rheology, Current Opinion ...

  20. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...ultracentrifuge. During the spin, the virions will form a band due to their high density, which can be collected...   Pro tip! An optional media exchange can be performed 12–18 hours post-transfection to help remove toxic...
Showing: 201 - 220 of 756 results