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Showing: 201 - 220 of 766 results

  1. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...ligase complex. Finally, when auxin is added in the form of auxin hormone indole-3-acetic acid (IAA), the...behave with a new protein, but you can make an informed choice!   References and Resources References...

  2. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...exogenously to measure a phenotypic response in the form of fluorescence or voltage indicators.   Viral... here!). The only issue with these GEVIs? They perform poorly in two-photon conditions, which are necessary...

  3. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...’t be lazy about splitting cells, instead try to form a routine. Twice a week often works for most fast-growing...

  4. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...transsynaptically). This is because the recombinant forms of these viruses typically used in research aren...after, we learned about organs and the jobs they perform. This became foundational later on when we studied...

  5. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ...case, the larva will metamorphose into its adult form. In all, it takes roughly 10 days for an egg to ...Some resources will direct you to the latest information about gene function, while others offer fly stocks...Flybase.org is absolutely essential. You can look up information about your fly gene using the search box in the... started. Any given gene’s webpage will have information on where the gene is expressed within the fly...website as well. Additionally, they have useful information on topics such as choosing the right balancer...

  8. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...MMEJ (microhomology-mediated end-joining)? MMEJ, a form of alternative end-joining, requires only very small...’s become clear that the power of this editing platform lies in the diversity of nucleases and their applications...

  9. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...the two halves are in close proximity, they will form the full FP and fluoresce. Split FPs can be used...

  10. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    Published
    Aug. 27, 2020, 1:15 p.m.
    ...protein interaction partners (PIPs), the FP fragments form the complete FP because the PIPs they’re tethered...to bring them into close proximity (Figure 1). Formation of the complete FP results in a fluorescent signal...selection of FP fragments and, preferably, some information about the structures of the tested PIPs. Many...fuse the FP fragments. In the absence of this information, you can fuse each FP fragment to the N- and ...your results. Unfortunately, without structural information, constructing appropriate controls may requires...

  11. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...synthetic oscillator, bistable network, stripe pattern-forming incoherent feed-forward loop. Find the plasmids...systems at Addgene! Schematic of the evolution platform to generate TEV protease variants. Catalysis by...the right places, which can provide misleading information on where exactly a structure or protein is located...dissolved at a higher concentration, and results in uniform NB biotinylation. Once induced, the NBs could be...developed fluorescently labeled piggyBac vectors for uniform and sustained expression of single or multiplexed...

  12. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    Published
    July 2, 2020, 12:40 p.m.
    ...chromatin, the complex of DNA and proteins that forms chromosomes. These long range DNA interactions are... are often detected with chromosome conformation capture (3C) based methods, such as 5C or Hi-C (Han et...generated with current 3C methods provide useful information about chromatin interactions, they are grainy...for answering more questions about chromatin conformations. The Xu lab presented three ways to use CAPTURE... capture of chromatin interactions The Xu lab performed a proof-of-concept experiment to show that CAPTURE...original CAPTURE method. Both CAPTURE methods outperformed other 3D chromatin methods such as ChIA-PET ...is detection of temporal changes in chromatin conformation, like those that occur during development and...

  13. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...expressed in E. coli, in mammalian cells, or in purified form, and didn’t need any added cofactors.      ...future MagLOV-based tools, just as light-induced conformational changes are the basis of many optogenetic tools...have collected a variety of data that might help formulate some hypotheses. They are excited to see other...

  14. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...secreted into the periplasmic space to be oxidized and form disulfide bonds (Frenzel et. al., 2013). If you’...applications. An antibody that performs well in immunohistochemistry may not perform well in a western blot. ... than mammalian cells, but mammalian cells can perform human-like post-translational modifications that... If that’s the case, a buffer exchange can be performed using a desalting column or an ultrafiltration...ones, consider increasing the number of washes performed during your harvest. Validation Recombinant antibodies.... This can be due to conformational changes in the antigen or lower detection sensitivity of the assay...S. Sarangapani, Protein aggregation, particle formation, characterization & rheology, Current Opinion ...

  16. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...ultracentrifuge. During the spin, the virions will form a band due to their high density, which can be collected...   Pro tip! An optional media exchange can be performed 12–18 hours post-transfection to help remove toxic...

  17. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...specify and assemble diverse arrays of neurons, forming the neuronal circuits that will later be refined...traditional cDNA overexpression: isoform-specific gene expression. Gene isoforms refer to mRNAs generated from...untranslated or coding regions. Typically, each gene isoform has a distinct transcriptional start site. Because...transcriptional start sites in order to better understand isoform-specific gene regulation — at least, more straightforward...straightforward than generating cDNA for each gene isoform individually. To highlight this advantage, they...neurons and synapses as well as long-term memory formation. The Bdnf gene possesses 9 distinct transcriptional...with CRISPRa, the Day lab singled out two Bdnf isoforms (I and IV) for investigation. Co-transduction ...

  18. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...vision. Practice drawing and writing everything in a form that is easily visible from the back of the room... vision. Organize your vision into a chalk talk format. Practice. Define your research vision A chalk...find, and how you will interpret and use the information. In contrast, a list of experiments or a laundry...Organize your research vision into the chalk talk format Once you have developed your research vision, you...most of your limited speaking time. Beyond the information you seek to convey in your chalk talk, there'...

  19. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...antibody-target protein immune complexes are allowed to form. Following incubation, beads are used to immobilize...-40 or triton-X, to preserve native protein conformations and interactions. For difficult to release proteins... in the lysis buffer, keep samples on ice, and perform the IP at 4oC. When choosing the capture antibody... recognizes the target protein in its native conformation. Frequently, the antibodies used for a standard... are completely removed. Wash steps should be performed even with a pre-cleared sample.  Eluting the target...and negative controls for an IP. When possible, perform the IP in parallel with both a sample known to ...order to identify any non-specific interactions, perform parallel IPs with beads only and an isotype control...

  20. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...structure. For example, cytoskeletal structures that form continuous lines in pre-expanded samples can sometimes...
Showing: 201 - 220 of 766 results