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Addgene

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Showing: 201 - 220 of 759 results

  3. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...working day, including appropriate MTAs and customs forms. Of course, ordering the plasmids is the easy part...

  4. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...ligase complex. Finally, when auxin is added in the form of auxin hormone indole-3-acetic acid (IAA), the...behave with a new protein, but you can make an informed choice! References and Resources References Bondeson...

  6. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...exogenously to measure a phenotypic response in the form of fluorescence or voltage indicators.   Viral... here!). The only issue with these GEVIs? They perform poorly in two-photon conditions, which are necessary...

  7. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...MMEJ (microhomology-mediated end-joining)? MMEJ, a form of alternative end-joining, requires only very small...’s become clear that the power of this editing platform lies in the diversity of nucleases and their applications...

  8. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...the two halves are in close proximity, they will form the full FP and fluoresce. Split FPs can be used...

  9. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...choice, you should perform a functional assay to make sure your protein isn’t forming inclusion bodies ...

  10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ... in spacer acquisition following infection, as a form of “bait” to identify new CRISPR-associated proteins...target binding and cleavage through a series of conformational changes in the Cas13a molecule. Like Cas9, ...

  11. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...changes in gene expression as new body sections are formed. In synthetic biology, FP timers might be able ...isolate particular organelles and their contents, or perform in situ hybridization experiments. In many cases...like the patterning of nascent tissues and the formation of limbs are correlated with changes in gene expression...and developmental timer of neural fiber bundle formation." Journal of Biological Chemistry 276.32 (2001...

  12. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    .... For instance, the fluorophore and quencher may form the base of the stem in an RNA stem-loop structure...and detection - since they frequently undergo conformational changes after target binding, aptamers are ...fluorescence-activated cell sorting (FACS) of E. coli transformed with aptamer libraries, the Jaffrey group selected...

  13. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...expressed in E. coli, in mammalian cells, or in purified form, and didn’t need any added cofactors.      ...future MagLOV-based tools, just as light-induced conformational changes are the basis of many optogenetic tools...have collected a variety of data that might help formulate some hypotheses. They are excited to see other...

  14. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...secreted into the periplasmic space to be oxidized and form disulfide bonds (Frenzel et. al., 2013). If you’...applications. An antibody that performs well in immunohistochemistry may not perform well in a western blot. ... than mammalian cells, but mammalian cells can perform human-like post-translational modifications that... If that’s the case, a buffer exchange can be performed using a desalting column or an ultrafiltration...ones, consider increasing the number of washes performed during your harvest. Validation Recombinant antibodies.... This can be due to conformational changes in the antigen or lower detection sensitivity of the assay...S. Sarangapani, Protein aggregation, particle formation, characterization & rheology, Current Opinion ...

  16. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...ultracentrifuge. During the spin, the virions will form a band due to their high density, which can be collected...   Pro tip! An optional media exchange can be performed 12–18 hours post-transfection to help remove toxic...

  17. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...specify and assemble diverse arrays of neurons, forming the neuronal circuits that will later be refined...traditional cDNA overexpression: isoform-specific gene expression. Gene isoforms refer to mRNAs generated from...untranslated or coding regions. Typically, each gene isoform has a distinct transcriptional start site. Because...transcriptional start sites in order to better understand isoform-specific gene regulation — at least, more straightforward...straightforward than generating cDNA for each gene isoform individually. To highlight this advantage, they...neurons and synapses as well as long-term memory formation. The Bdnf gene possesses 9 distinct transcriptional...with CRISPRa, the Day lab singled out two Bdnf isoforms (I and IV) for investigation. Co-transduction ...

  18. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...vision. Practice drawing and writing everything in a form that is easily visible from the back of the room... vision. Organize your vision into a chalk talk format. Practice. Define your research vision A chalk...find, and how you will interpret and use the information. In contrast, a list of experiments or a laundry...Organize your research vision into the chalk talk format Once you have developed your research vision, you...most of your limited speaking time. Beyond the information you seek to convey in your chalk talk, there'...

  19. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...structure. For example, cytoskeletal structures that form continuous lines in pre-expanded samples can sometimes...

  20. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...partnered with colleagues at other institutions to form a support network called the MDH CUREs Community...). But is it possible to bring the kinds of transformative educational experiences afforded to those fortunate.... Therefore, he decided to adopt a more focused format, where all students work on malate dehydrogenase...hockey coach all shaped his teaching style, with the former offering excellent training in improvisation and...NADH production.   CUREs are beginning to transform undergraduate education in the sciences by making...
Showing: 201 - 220 of 759 results