We narrowed to 214 results for: gnal

1. Plasmids 101: Repressible Promoters

   Type

   Blog Post

   Published

   Dec. 20, 2022, 2:15 p.m.

   ... working with Drosophila Terminators and PolyA Signals FLEx Vectors Resources at Addgene.org Tetracycline...

2. How to Design Your gRNA for CRISPR Genome Editing

   Type

   Blog Post

   Published

   Sept. 24, 2020, 1:15 p.m.

   ... that off-target effects did not overwhelm true signal in these assays (Dempster et al., 2019). Again,...

3. Antibodies 101: The Basics of Western Blotting

   Type

   Blog Post

   Published

   March 8, 2021, 2:41 p.m.

   ... the primary antibody and amplify the detection signal.   Analyzing the western blot Once visible, it’...

4. Technique: Probe Phage Genomes for Host Binding Proteins

   Type

   Blog Post

   Published

   Sept. 29, 2016, 2:30 p.m.

   ...lead to high background that obscures the desired signal. We have successfully corrected this by incubating...

5. Recombinase-based State Machines Enable Order-dependent Logic in vivo

   Type

   Blog Post

   Published

   July 28, 2016, 2:30 p.m.

   ...information regarding which regulatory proteins, signaling molecules, mutations, and environmental conditions...

6. Generating Mouse Models Using CRISPR/Cas9

   Type

   Blog Post

   Published

   July 12, 2016, 2:30 p.m.

   ... it gets exciting – the cell sends out an “SOS” signal and cellular repair mechanisms rush in to repair...

7. PiggyBac-ing Through the Genome Editing Field

   Type

   Blog Post

   Published

   May 31, 2016, 3:30 p.m.

   ...expressed has threshold effects, such as with signaling molecules or transcription factors, or when the...

8. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

   Type

   Blog Post

   Published

   Aug. 15, 2017, 1:24 p.m.

   ... volume. The consequence is that you can detect signal in thick specimens. See the video below for an ...

9. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

   Type

   Blog Post

   Published

   April 25, 2017, 2:30 p.m.

   ...absence of photoactivation resulting in an increased signal to noise ratio. PA-FPs with higher contrast ratios...

10. Special Delivery: Fluorophore Targeting for FRET Studies

    Type

    Blog Post

    Published

    July 19, 2016, 2:30 p.m.

    ...in energy transfer and thus, your specific FRET signal is unaffected  Due to these fundamental differences...

11. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type

    Blog Post

    Published

    Sept. 10, 2015, 2:30 p.m.

    ...of thumb, if you can detect a strong fluorescent signal (without antibody manipulation), then expression...

12. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type

    Blog Post

    Published

    Nov. 6, 2018, 1:12 p.m.

    ...cells via temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A, 2012. 109(27): p. E1848...

13. Antibody Validation for Flow Cytometry

    Type

    Blog Post

    Published

    Sept. 10, 2024, 1:15 p.m.

    ...explanations for RNAi not reducing the antibody signal include: The antibody is not selective The knockdown...

14. CRISPR 101: Cytosine and Adenine Base Editors

    Type

    Blog Post

    Published

    Feb. 13, 2025, 2:15 p.m.

    ... The Liu lab modified the nuclear localization signals and codon usage BE4 to create BE4max and AncBE4max...