We narrowed to 216 results for: gnal

1. Using Phosphoserine to Study Protein Phosphorylation

   Type

   Blog Post

   Published

   June 23, 2016, 2:30 p.m.

   ...particularly interested in the intersection of cell signaling, metabolism, and the mighty mitochondrion. You...

2. Evolution of Lab Techniques

   Type

   Blog Post

   Published

   June 21, 2016, 2:30 p.m.

   ...result can be quantified by the strength of the signal in comparison to a control sample. Southern blotting...

3. Plasmids 101: Repressible Promoters

   Type

   Blog Post

   Published

   Dec. 20, 2022, 2:15 p.m.

   ... working with Drosophila Terminators and PolyA Signals FLEx Vectors Resources at Addgene.org Tetracycline...

4. Antibodies 101: The Basics of Western Blotting

   Type

   Blog Post

   Published

   March 8, 2021, 2:41 p.m.

   ... the primary antibody and amplify the detection signal.   Analyzing the western blot Once visible, it’...

5. How to Design Your gRNA for CRISPR Genome Editing

   Type

   Blog Post

   Published

   Sept. 24, 2020, 1:15 p.m.

   ... that off-target effects did not overwhelm true signal in these assays (Dempster et al., 2019). Again,...

6. Technique: Probe Phage Genomes for Host Binding Proteins

   Type

   Blog Post

   Published

   Sept. 29, 2016, 2:30 p.m.

   ...lead to high background that obscures the desired signal. We have successfully corrected this by incubating...

7. Recombinase-based State Machines Enable Order-dependent Logic in vivo

   Type

   Blog Post

   Published

   July 28, 2016, 2:30 p.m.

   ...information regarding which regulatory proteins, signaling molecules, mutations, and environmental conditions...

8. Generating Mouse Models Using CRISPR/Cas9

   Type

   Blog Post

   Published

   July 12, 2016, 2:30 p.m.

   ... it gets exciting – the cell sends out an “SOS” signal and cellular repair mechanisms rush in to repair...

9. PiggyBac-ing Through the Genome Editing Field

   Type

   Blog Post

   Published

   May 31, 2016, 3:30 p.m.

   ...expressed has threshold effects, such as with signaling molecules or transcription factors, or when the...

10. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type

    Blog Post

    Published

    Aug. 15, 2017, 1:24 p.m.

    ... volume. The consequence is that you can detect signal in thick specimens. See the video below for an ...

11. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type

    Blog Post

    Published

    April 25, 2017, 2:30 p.m.

    ...absence of photoactivation resulting in an increased signal to noise ratio. PA-FPs with higher contrast ratios...

12. Special Delivery: Fluorophore Targeting for FRET Studies

    Type

    Blog Post

    Published

    July 19, 2016, 2:30 p.m.

    ...in energy transfer and thus, your specific FRET signal is unaffected  Due to these fundamental differences...

13. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type

    Blog Post

    Published

    Sept. 10, 2015, 2:30 p.m.

    ...of thumb, if you can detect a strong fluorescent signal (without antibody manipulation), then expression...

14. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type

    Blog Post

    Published

    Nov. 6, 2018, 1:12 p.m.

    ...cells via temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A, 2012. 109(27): p. E1848...

15. Antibody Validation for Flow Cytometry

    Type

    Blog Post

    Published

    Sept. 10, 2024, 1:15 p.m.

    ...explanations for RNAi not reducing the antibody signal include: The antibody is not selective The knockdown...

16. CRISPR 101: Cytosine and Adenine Base Editors

    Type

    Blog Post

    Published

    Feb. 13, 2025, 2:15 p.m.

    ... The Liu lab modified the nuclear localization signals and codon usage BE4 to create BE4max and AncBE4max...