We narrowed to 229 results for: mel

1. Using Video to Share Your Science: We Share Science

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   Blog Post

   Published

   Nov. 3, 2016, 2:30 p.m.

   ...  “Singing” Fish Rely on Circadian Rhythm and Melatonin for the Timing of Nocturnal Courtship Vocalization...

2. Getting the Most from Your Lentiviral Transduction

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   Blog Post

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   April 7, 2016, 2:30 p.m.

   ... yeast, fungi, or bacteria, mycoplasma can be extremely hard to detect with levels reaching 108 cells ...

3. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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   Blog Post

   Published

   April 9, 2019, 1:13 p.m.

   .... In fact, for several FRET biosensors (yellow cameleon 3.60, EPAC, and Galphai), the C-terminus and N-terminus...

4. Plasmids 101: Biotinylation

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   Blog Post

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   Nov. 15, 2018, 1:50 p.m.

   ...molecule of biotin. Biotin and avidin have an extremely strong affinity for one another. Actually the ...

5. Hot Biosensors 2022: Year-End Roundup

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   Blog Post

   Published

   Dec. 6, 2022, 2:15 p.m.

   ...acssensors.1c02201. LiLac sensor for lactate Stop and smell the flowers - or rather, the lactate! The latest...

6. CRISPR 101: Validating Your Genome Edit

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   Blog Post

   Published

   Nov. 3, 2022, 12:15 p.m.

   ...This post was originally written by Melina Fan and updated Nov 3, 2022 by Susanna Stroik. You’ve designed...

7. Transferable Skills Guide: Conflict Resolution

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   Blog Post

   Published

   Jan. 4, 2022, 2:15 p.m.

   ...other just for the sake of disagreeing. It can be extremely toxic in a lab or work environment if left unchecked...

8. Plasmids 101: Expressing shRNAs for RNA Interference

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   Blog Post

   Published

   June 17, 2025, 1:15 p.m.

   ...Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998). Potent and specific genetic interference...

9. Antibodies 101: Normalization and Loading Controls for Western Blots

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   Blog Post

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   April 8, 2025, 1:15 p.m.

   ...detection range, band intensity measurements become extremely susceptible to error, since tiny changes in intensity...

10. Hot Plasmids: Summer 2024

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    Blog Post

    Published

    Aug. 27, 2024, 1:15 p.m.

    ...Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., … & Weissman, J. S. (2021). Genome-wide...

11. Antibodies 101: Introduction to Gating in Flow Cytometry

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    Blog Post

    Published

    April 4, 2024, 1:15 p.m.

    ...of my previous experiments where I harvested a melanoma tumor from a C57BL/6 mouse and analyzed the tumor-infiltrating...

12. An Integrin Antibody Toolkit from IPI

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    Blog Post

    Published

    March 28, 2024, 1:15 p.m.

    ...This pocket-binding property in an antibody is extremely rare. While many function-blocking integrin antibodies...

13. Multiple Plasmids at a Low Price: Kits from Addgene

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    Blog Post

    Published

    Sept. 14, 2016, 2:30 p.m.

    ... Huang, Shijie, Xuechen Zhu, and Charles E. Melançon III. "Detection and Quantification of Ribosome...

14. Site Directed Mutagenesis by PCR

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    Blog Post

    Published

    Aug. 2, 2016, 2:30 p.m.

    ...introduced anywhere in the plasmid (albeit at extremely low frequency), and these could interfere with...

15. Evolution of Lab Techniques

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    Blog Post

    Published

    June 21, 2016, 2:30 p.m.

    ...and behavior.     References 1. Southern, Edwin Mellor. "Detection of specific sequences among DNA fragments...

16. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    Blog Post

    Published

    May 10, 2016, 2:30 p.m.

    ...CRISPR may be the way to accomplish this tough job! Kamel Khalili’s lab at Temple University has demonstrated...

17. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    Blog Post

    Published

    March 1, 2016, 3:30 p.m.

    ...recommends an overlap of 15-40 bp, with a primer melting temperature greater than 48℃. Both Snapgene and...

18. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    Blog Post

    Published

    April 24, 2015, 2:39 p.m.

    ...labeling for neural circuit analysis in Drosophila melanogaster. Hadjieconomou D, Rotkopf S, Alexandre C, Bell...

19. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type

    Blog Post

    Published

    April 5, 2018, 12:42 p.m.

    ...Miura et al. (2015) developed the ivTRT method to ameliorate this problem. Briefly, you design your donor ...

20. Technologies Enabled by NanoLuc® Luciferase

    Type

    Blog Post

    Published

    Feb. 8, 2018, 12:17 p.m.

    ...intracellular Ca++ release with an optogenetic receptor, melanopsin, requiring brief exposure to 470nm blue light...