We narrowed to 794 results for: mpl

...Palindromic Repeats. That contrast of simplicity and complexity is reflected in the biology, too. CRISPR..., and the spacer sequence is used to target complementary DNA sequences. In laboratory settings, an sgRNA...whose sequence is not checked. Assuming full complementarity, its sequence matches the guide sequence. PAM...into a single strand that folds back on itself, simplifying the CRISPR-Cas9 system for laboratory applications...either crRNA or sgRNA). Cas9 requires perfect complementarity in this region between the guide and target...post on the structural biology of Cas9. Want a simpler guide to keep at your desk? We’ve got a CRISPR ...

...GPCRs (Takeda S et al., 2002) and many downstream complexes involved, it can be difficult for scientists to...in sight (Olsen RHJ et al., 2020). A transducer complex consists of a trimer of three subunits: alpha, ...collectively known as a heterotrimeric G protein. This complex converts a signal received by a GPCR on the cell...is being able to identify specific transducer complexes that are activated in response to a ligand binding...inactive, these subunits are found in a trimeric complex bound to the intracellular surface of the GPCR....Following activation in response to the ligand, the complex will disassociate, resulting in a decreased GFP2...screening across the transducerome, to gain a more complete understanding of a GPCR mediated response.  Acknowledgements...

...communicating science to laypeople.  Laypeople are simply non-experts in the field of science you are communicating...use-case (definition) in a specific field.  For example, while “PCR test” has recently transitioned from...PCR test” to mean “a test that uses a biological sample to look for presence of a virus.” A scientist would...sequence that uses trprimers and enzymes that will amplify a sequence only if present.” You can see how you...the possibilities are endless.    Activity Example audience  Talking about your career as a scientist... podcast about genetics using dog breeds as an example adults 45-55 yrs old who breed or are interested...online patient/caregiver communities  Table 1: Examples of defining your audience for science communication...

...will result in more engaged, successful employees who can accomplish great things.   To read more on this...time for that and you won’t be as productive. For example, the many resources available on the Addgene website...enough to do…you are not delegating enough. For example, if we need to develop a new plasmid collection...duplicated. With each component of the project completed by an individual who is a local expert in that...result is high quality and the entire process is completed more efficiently. Listen to our podcast interview...desired outcomes. Concern yourself with what is accomplished, rather than detailing how the work should be...

...management, and how does it relate to science? In the simplest of terms, a software product manager helps ensure...manager works with software developers to help implement solutions for the stakeholders. Finally, software...all fits in with science. Well, I’ll give you an example. At my previous company, Ginkgo Bioworks, our software...backgrounds, most did not. It was my job to translate complex lab operations workflows into a format the developers... to solve is clear, but the solution is more complicated. You’ll need to be creative to be able to work...working with data, working with people, working with complex processes Arm yourself with knowledge of software...catching up in the morning! 9:00 AM--Technical implementation review. The developers discuss which front-...

...minipreps for small batches of samples. As soon as that process is complete, QC scientists prepare Sanger...mutation.  Once the samples arrive, each group of plates will have select samples prepared and screened...available. Plasmids are continually added as they complete QC and you can find all available plasmids listed...screened by NGS to spot check the samples, the rest will be put into a status we call “Archived.” This status...for all several thousand plasmids at once. Any samples that are requested by scientists, will immediately...

...achievement, the complexity of the device increases, as does the price. With this increased complexity comes the...very difficult to adapt the device to one's own samples and limits the use of microscopy in open science...teleporter," since a part developed in Germany, for example, can also be printed quickly in Madagascar. Platforms...a move towards more open research. The EU, for example, is calling for the opening of sources within the...hardware.    Conclusion Addgene is a great example of how sharing of data and molecular tools can ...Nibha Akireddy, Chew Chai, Manu Prakash, "Squid: Simplifying Quantitative Imaging Platform Development and...

... For example, setting up your pipette tips to match your plate layout can help you track samples as you...Identifying the Issue You can’t fix an experiment by simply running it unchanged over and over again, and the...change variables to fix a failed experiment.  For example, I used to constantly underestimate time needed...was not trying even harder to start earlier, but simply moving all my prep work to the day before, which... and make a checkmark next to each step as you complete it. Yes, this can be incredibly tedious, but it...remember what's been added and what hasn't. Developing simple habits like these can consistently reduce errors...

...Chemical agents, temperature, and light are all examples of factors that can lead to the induction of a... these three types of inducible promoters, and examples of each type. Many of these promoter systems are...promoter and ceases repression of the target gene. A simplified lac inducible system is found in many bacterial...transcription to begin. In addition to arabinose, cAMP complexed with cAMP activator protein (CAP) can also stimulate...represses pBad, decreasing promoter leakiness.   Other examples of chemically induced promoters include positive...in plant research. Promoter Subtype Promoter Example Activator Addgene Plasmid/Kit Alcohol-regulated...temperatures but can be induced by heat or cold exposure. Examples include the heat shock-inducible Hsp70 or Hsp90...

... range from a defined pathway or reaction to a complex event such as cancer or even aging. For both a ...differences in these approaches can be found. As an example, say you are considering either a cellular screen...libraries targeting all known genes. This is a perfect example of an unbiased, reverse screen – all known genes...possessing a specific mutation or phenotype. For example, a drug screen may be performed on a cell line ...patients may respond to drug treatment. Another example is using a mouse model of a particular disease ...previously defined disease phenotype. These screens employ the same downstream manipulations as a forward ...that do not affect the function of interest. An example where a selection would work is if you are trying...

...results? Or considered why DpnI will degrade your template DNA from a PCR reaction but not the newly synthesized... Methylases involved with these processes (for example Dam and Dcm methylases) are independent from the...conversely, activating enzymes such as DpnI. For example, cleavage by XbaI may be blocked due to methylation...no methyltransferases in the PCR reaction). The template plasmid, on the other hand, should be derived ...with DpnI, only the non-mutated and methylated template is destroyed leaving behind a pool of mutated ...those sites will be methylated. Using the same example plasmid as above (which has the XbaI site blocked...methylation by altering your choice of bacteria. For example, if you must use a restriction site that will be...

...this complex holds a pair of DNA ends together, forming a paired end complex The paired end complex then...repair, as there is no requirement for a repair template (sister chromatid or homologue) or extensive DNA...together, thus repairing the break. This is a simplified, streamlined version of this pathway and does... factors can also influence NHEJ activity. For example, repressing RecA and overexpressing the NHEJ machinery...NHEJ-dependent repair (“pop-in” insertion) provided a template containing compatible overhangs is available. ...

...predicted structure of your antibody-antigen complex as a template. This structural framework then guides the...equipment on a lab bench. The best part? Less complicated math. Bioengineering is a field that has been...methods. (A) Basic structure of an antibody. (B) Examples of engineered antibodies, including chimerization...directed evolution Directed evolution is a method employed to rapidly change a protein towards a specific...achieve whatever goal you’re aiming for. When you complete each cycle, your best-performing mutant is usually...different primers. While error-prone PCR is usually employed to mutate whole antibody sequences, degenerate...specific functionality you’re looking for. For example, if you were aiming to increase an antibody’s binding...

...Level-1). Other possible tools, lentiviruses for example, are not approved for use in vivo use at the University...hippocampus and cortex) within the mouse brain (4). An example from the cortex is outlined in the table below....is possible to use AAV constructs with self-complementing structure (scAAV) (7). As single stranded DNA...mapping of circuits In our lab, we use AAV-DREADD complexes to study neural function. Often the reporter proteins... proteins associated with these complexes are so robustly expressed that anatomical assessments can be...be easily performed too. While I implied that axoplasmic transport of AAVs is a potential problem in many... E. Monahan, and Richard J. Samulski. "Self-complementary recombinant adeno-associated virus (scAAV) vectors...

...track of past accomplishments. Follow all the rules of good resume writing. Include examples of concrete.... When I am speaking at a new institution, for example, I will often see a whole bunch of notifications...about (or how good the beer was at that place, for example). This is also a great way to keep track of how... and trolls. People don’t like to connect with complete strangers as it borders on my first rule of networking...’m asked a lot about requesting connections to complete strangers for informational interviews. Many have...concrete accomplishments in both the technical and “plus skills” areas. Include a professional picture. It doesn...

...presentation, lost in a sea of data, my mind went completely blank. After several seconds of truly panicked...honest, if my paper hadn’t been ridiculously more complex than the one before it, I don’t think I would have...getting too nervous or having a memory lapse, I simply pause and take a deep breath before the panic sets... panicked state, only option one would let me accomplish my goal.   As a scientist, public speaking isn't...dumpster noises were just last week!  Nerves It’s completely normal to be anxious, nervous, and/or apprehensive...make a strategy that can help. Even something as simple as telling yourself, “I can stop and check my notes... in its most difficult form. You’re presenting complex data, to a group of peers who are there to provide...

...interest. Furthermore, fluorophore variants (for example green and red fluorescent proteins) allow simultaneous...properties or trafficking of the fusion protein (Kimple et al., 2013). We found that the fluorescent proteins...needed, the use of HA and other short epitope tags (Kimple et al., 2013) could help improve the transport ...mCherry (top) or HA tag (bottom). The images show examples of dendrites (blue shades) in the monkey amygdala... not generalizable to all fusion proteins. For example, we saw that another type of DREADD, hM3Dq, is ...Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope...50:2801–2813 . https://doi.org/10.1111/ejn.14429 Kimple ME, Brill AL, Pasker RL (2013) Overview of Affinity...

... do as genetic circuits become larger and more complex? Voigt: If you look at all the products of biology... on genes at different times. It requires more complex synthetic networks. Addgene: What hurdles did you... perspective and you can layer them for more complicated functions. Those things are easy with CRISPR..... There haven’t been enough genetic parts for complexity. We have characterized almost 600 terminators...of a cell. The objective is to give people the complete toolbox for taking on very large genetic engineering...biotechnology today are things like butanol - very simple molecules - a few carbons and oxygens. Nothing ...just using what biology has given us. Wood is a complicated material and we can access it, but we are stuck...

...Although these multi-component systems can be complicated to implement, they can be highly specific with a small...Various Few Complicated Non-natural amino acids 1 Various Many Complicated   Applications...ligands to the same sample to label sub-populations of your protein of interest. For example, the Cohen lab... assembly-regulating proteins in vitro reveals complex events. a) Micrographs of a fluorescently-labeled..., biotin, or magnetic beads. In one innovative example, the Zhang lab used SNAP-tag to decorate synthetic...

...binding site (PBS) complementary to the genome and a reverse transcriptase (RT) template including the desired...reverse transcriptase template, starting with about 10–16 nucleotides. For longer templates, it is even more...primer binding site (PBS) and reverse transcriptase template (RTT). Created with BioRender.com. The first...encourage the cell to use the edited strand as a template during DNA repair. Test multiple nick sites, starting...Nureki, O. (2014). Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA. Cell, 156(5), 935...