We narrowed to 322 results for: nans

1. Addgene's Top 10 Blog Posts of 2015

   Type

   Blog Post

   Published

   Dec. 7, 2015, 6 p.m.

   ...Blue-White Screening Getting a lot of empty vector contaminants in your cloning experiments and tired of having...

2. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

   Type

   Blog Post

   Published

   Oct. 29, 2015, 2:30 p.m.

   ...generic term for any extrachromsomal hereditary determinant”. His proposal, however, was basically ignored...

3. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

   Type

   Blog Post

   Published

   April 7, 2015, 4:21 p.m.

   ...University of Western Ontario studying plant-based recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt...

4. Advice for Moving Into Sales After Your Science Postdoc

   Type

   Blog Post

   Published

   Aug. 21, 2014, 3:15 p.m.

   ...marketing gives you a great introduction to the financial world. It teaches you about selling products which...

5. Hot Plasmids: FRET-Based Biosensors

   Type

   Blog Post

   Published

   May 6, 2014, 1:07 p.m.

   ...contribution to the field is a series of fluorescence resonance energy transfer (FRET)-based calcium biosensors...

6. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

   Type

   Blog Post

   Published

   July 21, 2017, 12:48 p.m.

   ... a means of altering cell fates and generating dominant phenotypes., Development 118, 401–15 (1993). PubMed...

7. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

   Type

   Blog Post

   Published

   June 20, 2017, 2:30 p.m.

   ...Zolotukhin S. A simplified purification protocol for recombinant adeno-associated virus vectors. Mol Ther Methods...

8. Cultivating Community Science at BosLab

   Type

   Blog Post

   Published

   Nov. 2, 2017, 1:35 p.m.

   ...experiments. For example, Dave Grawoig is using nanopore sequencing at BosLab to perform proof-of-concept...

9. Plasmids 101: NGS Quality Control for Pooled Libraries

   Type

   Blog Post

   Published

   Oct. 26, 2017, 1:59 p.m.

   ...proper use requires careful amplification and maintenance of good representation. Addgene wishes you luck...

10. Celebrate National Book Month with Addgene

    Type

    Blog Post

    Published

    Oct. 3, 2019, 12:47 p.m.

    ... PhD, majored in Biochemistry or International Finance. A book club could lead to smart, sometimes contentious...

11. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type

    Blog Post

    Published

    May 21, 2019, 12:10 p.m.

    ...reporters have been developed, such as the Forster resonance energy transfer (FRET)-based executioner caspase...

12. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type

    Blog Post

    Published

    Dec. 20, 2018, 1:15 p.m.

    ... PCSK9 paper, though not featuring a clinical pregnancy, was rejected by at least one academic journal...

13. Summer SciComm Series: A PhD in Science Communication

    Type

    Blog Post

    Published

    Aug. 23, 2022, 1:15 p.m.

    ...little tricks our minds perform to avoid cognitive dissonance (Kunda, 1990). More specifically, they were engaging...

14. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type

    Blog Post

    Published

    Feb. 9, 2021, 2:15 p.m.

    ...Wuchty S, Jones BF, Uzzi B (2007) The Increasing Dominance of Teams in Production of Knowledge. Science 316...

15. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type

    Blog Post

    Published

    March 24, 2016, 2:30 p.m.

    ...of this repair bias is a fascinating (and yet unanswered) question. Our recent paper (Richardson et al...

16. Plasmids 101: Mammalian Vectors

    Type

    Blog Post

    Published

    March 25, 2014, 3:15 p.m.

    ...is typically more (double) than that used for maintenance of a transfected cell line.  Keep these tips ...

17. Why and how to start studying chromatin (with Addgene's help)

    Type

    Blog Post

    Published

    June 12, 2018, 11:40 a.m.

    ...JB Mol Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive...

18. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type

    Blog Post

    Published

    June 1, 2017, 2:30 p.m.

    ...fluorescent protein in the host cell, which is a key determinant of the practical brightness. Therefore, practical...

19. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type

    Blog Post

    Published

    May 11, 2017, 2:30 p.m.

    ...Comprehensive Set of MAPK1/ERK2 Missense Mutants. Brenan L et al. Cell Reports, 2016. PubMed PMID: 27760319...

20. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type

    Blog Post

    Published

    Jan. 30, 2020, 2:15 p.m.

    ...minimized with high powered research designs. Unanticipated discoveries Finally, there are good reasons ...