We narrowed to 264 results for: nif

1. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

   Type

   Blog Post

   Published

   Oct. 11, 2018, 12:30 p.m.

   ...-Crick base pair substrates, it demonstrates significant activity on some mismatch-containing pairings...

2. Tips to Make the Most of a Scientific Conference

   Type

   Blog Post

   Published

   May 23, 2019, 12:26 p.m.

   ... Jennifer Tsang...

3. 3D Printing Meets CRISPR Cas9

   Type

   Blog Post

   Published

   Oct. 17, 2017, 12:55 p.m.

   ...explaining CRISPR technology to 4th graders, check out Jennifer Doudna’s new book A Crack in Creation. Making...

4. Hot Plasmids and Viral Preps - May 2021

   Type

   Blog Post

   Published

   May 18, 2021, 1:15 p.m.

   ...10.1101/2021.01.30.428974   The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR...

5. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

   Type

   Blog Post

   Published

   Feb. 4, 2020, 2:15 p.m.

   ... Jennifer Tsang...

6. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

   Type

   Blog Post

   Published

   April 4, 2019, 12:53 p.m.

   ...Mobile-CRISPRi plasmids at Addgene! References Auchtung, Jennifer M., et al. "Biology of ICEBs1, an integrative ...

7. Tips for Using BLAST to Verify Plasmids

   Type

   Blog Post

   Published

   May 29, 2014, 1:29 p.m.

   ...Organism box. This small piece of information can significantly reduce your wait time for blastn, blastp, and...

8. Plasmids 101: Choosing an Antibiotic Resistance Gene

   Type

   Blog Post

   Published

   Oct. 10, 2023, 1 p.m.

   ...wall synthesis step during cell division. This is nifty because the recovery period after transformation...

9. An Introduction to Adenovirus

   Type

   Blog Post

   Published

   Oct. 3, 2023, 1:15 p.m.

   ...basic research, like other viral vectors, though significant challenges remain (Wold & Toth, 2013).  While...

10. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type

    Blog Post

    Published

    Oct. 4, 2018, 12:44 p.m.

    ..., confirming again that sgRNA design plays a significant role in library efficiency. Try the Dolcetto ...

11. AAVs in Retinal Gene Therapy

    Type

    Blog Post

    Published

    July 31, 2018, 12:55 p.m.

    ...reported results are encouraging. Importantly, a significant amount of basic research is being done to further...

12. DNA Purification Without a Kit

    Type

    Blog Post

    Published

    Feb. 11, 2020, 2:15 p.m.

    ...have advantages: they are convenient and provide uniform, consistent results. But they are also less accessible...

13. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    Published

    Feb. 27, 2018, 2:04 p.m.

    ...antibody-containing hybridoma supernatant is only $40, significantly cheaper than commercially available antibodies...

14. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type

    Blog Post

    Published

    Aug. 9, 2016, 2:30 p.m.

    ...(ESCs). Your ChIP-seq finds just over 13,000 significant binding peaks for NANOG in the human genome. ...

15. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type

    Blog Post

    Published

    March 17, 2016, 2:30 p.m.

    ...polymerase activity, whereas some ratios result in significantly reduced activity (4). To ensure that you end...

16. Cpf1: A New Tool for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Oct. 14, 2015, 2:30 p.m.

    ... Direct synthesis of these crRNAs should be significantly cheaper than that of the ~100 nt crRNA/tracrRNA...

17. A Career in Grants: How To Become a Grant Professional

    Type

    Blog Post

    Published

    Aug. 20, 2015, 2:30 p.m.

    ...grant seekers to use online applications with significant character limits for the written portion of the...

18. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type

    Blog Post

    Published

    July 29, 2025, 1:15 p.m.

    ... Panda, D., Baig, M. J., & Molla, K. (2025). A unified protocol for genome editing in monocot and dicot...

19. QC Sequencing Technologies at Addgene

    Type

    Blog Post

    Published

    July 15, 2025, 1:15 p.m.

    ...This post was written by Alyssa Shepard, with significant contributions from Alyssa Cecchetelli, Cary Valley...

20. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type

    Blog Post

    Published

    July 8, 2025, 1:15 p.m.

    ...This post was written by Alfonso Timoneda, with significant contributions from Robert Hurt and Mohamed Soufi...