We narrowed to 265 results for: nif

1. Tips to Make the Most of a Scientific Conference

   Type

   Blog Post

   Published

   May 23, 2019, 12:26 p.m.

   ... Jennifer Tsang...

2. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

   Type

   Blog Post

   Published

   April 4, 2019, 12:53 p.m.

   ...Mobile-CRISPRi plasmids at Addgene! References Auchtung, Jennifer M., et al. "Biology of ICEBs1, an integrative ...

3. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

   Type

   Blog Post

   Published

   Oct. 11, 2018, 12:30 p.m.

   ...-Crick base pair substrates, it demonstrates significant activity on some mismatch-containing pairings...

4. Twenty Years of Addgene Sharing: CRISPR

   Type

   Blog Post

   Published

   April 18, 2024, 1:15 p.m.

   ...other countries, from Japan to Australia, are significant depositors and requestors. CRISPR tools are developed...

5. Of Myc and Men

   Type

   Blog Post

   Published

   Jan. 19, 2023, 2:15 p.m.

   ... the N-terminal half of the antigen differs significantly between human and the other species, suggesting...

6. 3D Printing Meets CRISPR Cas9

   Type

   Blog Post

   Published

   Oct. 17, 2017, 12:55 p.m.

   ...explaining CRISPR technology to 4th graders, check out Jennifer Doudna’s new book A Crack in Creation. Making...

7. Tips for Using BLAST to Verify Plasmids

   Type

   Blog Post

   Published

   May 29, 2014, 1:29 p.m.

   ...Organism box. This small piece of information can significantly reduce your wait time for blastn, blastp, and...

8. DNA Purification Without a Kit

   Type

   Blog Post

   Published

   Feb. 11, 2020, 2:15 p.m.

   ...have advantages: they are convenient and provide uniform, consistent results. But they are also less accessible...

9. AAVs in Retinal Gene Therapy

   Type

   Blog Post

   Published

   July 31, 2018, 12:55 p.m.

   ...reported results are encouraging. Importantly, a significant amount of basic research is being done to further...

10. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    Published

    Feb. 27, 2018, 2:04 p.m.

    ...antibody-containing hybridoma supernatant is only $40, significantly cheaper than commercially available antibodies...

11. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type

    Blog Post

    Published

    Oct. 10, 2023, 1 p.m.

    ...wall synthesis step during cell division. This is nifty because the recovery period after transformation...

12. An Introduction to Adenovirus

    Type

    Blog Post

    Published

    Oct. 3, 2023, 1:15 p.m.

    ...basic research, like other viral vectors, though significant challenges remain (Wold & Toth, 2013).  While...

13. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type

    Blog Post

    Published

    Oct. 4, 2018, 12:44 p.m.

    ..., confirming again that sgRNA design plays a significant role in library efficiency. Try the Dolcetto ...

14. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type

    Blog Post

    Published

    Aug. 9, 2016, 2:30 p.m.

    ...(ESCs). Your ChIP-seq finds just over 13,000 significant binding peaks for NANOG in the human genome. ...

15. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type

    Blog Post

    Published

    March 17, 2016, 2:30 p.m.

    ...polymerase activity, whereas some ratios result in significantly reduced activity (4). To ensure that you end...

16. Cpf1: A New Tool for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Oct. 14, 2015, 2:30 p.m.

    ... Direct synthesis of these crRNAs should be significantly cheaper than that of the ~100 nt crRNA/tracrRNA...

17. A Career in Grants: How To Become a Grant Professional

    Type

    Blog Post

    Published

    Aug. 20, 2015, 2:30 p.m.

    ...grant seekers to use online applications with significant character limits for the written portion of the...

18. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type

    Blog Post

    Published

    Oct. 13, 2022, 1:45 p.m.

    ...post was originally written by Joel McDade and significantly updated in 2022 by Susanna Stroik.  The advent...

19. Bioinformatics at Addgene

    Type

    Blog Post

    Published

    July 14, 2022, 1:15 p.m.

    ...without having to translate or convert programs to a uniform language.  The modern architecture of Nextflow ...

20. Viral Vectors 101: Pseudotyping

    Type

    Blog Post

    Published

    April 20, 2021, 2:30 p.m.

    ... Jennifer Tsang...