...10x magnification. To determine the final magnification of your image, multiply the magnification of your...lens which magnifies the image, then the ocular lens, where the image is further magnified before it finally...for now) is the magnification power, such as 4x, 10x, or 20x. A 4x objective magnifies the image four ...10x objective magnifies it 10 times, and so on. The ocular lens also provides magnification and the power...most important properties of a microscope are magnification (the ability to make an image larger) and resolution...reaches your eyes. These lenses determine the magnification of the image and the resolution your microscope...performs essentially the same task but has different magnifying powers. When you look at the side of an objective...

...for options if the recombinant band makes up a significant proportion of the DNA pool. Last Update: August...taking place during electroporation. Arcing would manifest as a loud pop often accompanied by some light ... off entirely if the column or reagents are significantly overloaded. If you find that your pellet outstrips...

...the NanoDrop, if two consecutive samples have significantly different concentrations, it is possible that...between samples if they are expected to vary significantly in concentration. Note: DNA dissolved in water...

...homology region. Hairpins in this region can significantly reduce the efficiency of two homologous ends...gel to check for size and yield. If there are significant amounts of undesired product, gel-purify DNA ...

...recipient plasmid + insert plate should have significantly more colonies than the recipient plasmid alone...recipient plasmid self-ligation, you will see significantly more colonies when you add ligase. If you do...

...recipient plasmid + insert plate should have significantly more colonies than the recipient plasmid alone...recipient plasmid self-ligation, you will see significantly more colonies when you add ligase. If you do...

...freezing (5-6 times). Make sure that you see one uniform solution, and there are no layers present. Be sure...

...available. Unlike the plastic tray, this will not significantly reduce the UV, but will protect the UV box from...

...or enzymes) that cuts within the insert, but significantly off center (ideally around 1/3 of the way from...

...volume as your samples. If your samples are significantly higher density than water, then you may need...

... *Pro-Tip* Commercial competent cells range significantly in their transformation efficiency. The lowest...

...-Tip* The optimal ratio of DNA:PEI may vary significantly and should be empirically determined for your...

... to collect the solution, or you will lose a significant amount of your virus. When the first fraction...