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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 363 results for: nsf

Showing: 201 - 220 of 363 results

  1. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ... if these do not provide clarity, a wet lab transformation experiment will ultimately inform whether your...classification and identification based on replication and transfer systems and host taxonomy. Frontiers in Microbiology...

  3. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...drafts of the manuscript. This is the time to transform the document into something cohesive-- change ...Thompson, and Thomas D. Gilmore. "Histone acetyltransferases and histone deacetylases in B-and T-cell ...

  4. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...are referred to as methods of ‘horizontal gene transfer’. Resistance can also arise from random mutations...either through random mutation or horizontal gene transfer, means to defend themselves from these naturally...

  5. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...site. (c) The pCAG-EGxxFP target plasmid was co-transfected with pX330 plasmids containing sgRNA sequences...efficacy of your gRNAs is then a simple matter of transfecting HEK293T cells with your modified pCAG-EGXXFP...

  6. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...delivered as any type of small RNA and can occur via transfection or viral delivery of a plasmid. RNAi is an umbrella...mature siRNA. siRNAs are delivered via transient transfections and are handy for quick in vitro experiments...

  7. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...second-generation system as an example.  The lentiviral transfer plasmid encodes your insert of interest, flanked...facilitates viral entry into the host cell.  Transfecting these three plasmids into cells enables the ...

  8. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...as effective NIR fluorescence in transiently transfected live HeLa cells with no supply of exogenous BV...and after normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0...

  9. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...Efficient gene transfer in C. elegans: extrachromosomal maintenance and integration of transforming sequences...

  10. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...identical targeting vectors delivered through transfection of naked DNA. Other investigators who had been...then packaged into AAV virions via transient transfection of 293T cells with helpers, and used to infect...

  11. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...aptamers are used to track cellular processes by transforming cells with plasmids encoding aptamer-tagged ...expression vectors to create a library. Bacteria were transformed, and then sorted by fluorescence-activated cell...

  12. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ... plasmid. The ssDNA template is generated by transforming a backbone phagemid vector into an E. coli strain...to remove residual starting template. After transformation and harvest, the library is ready for use. ...

  14. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...Propagation of ccdB-containing plasmids Hte   High transformation efficiency deoR constitutive expression of...+), λ– EPI300   Cloning large inserts and transformation of large plasmids. F- mcrA Δ(mrr-hsdRMS-mcrBC...

  15. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    .../cJ and CBA/J mice. AAV1-X1 was developed by transferring the 7-mer peptide region from variable region... viral vectors for functional intravenous gene transfer throughout the non-human primate brain. Nature...selection yields AAV variants for widespread gene transfer to the adult brain. Nature Biotechnology, 34(2...

  16. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ... (AAV) Serotype 9 Provides Global Cardiac Gene Transfer Superior to AAV1, AAV6, AAV7, and AAV8 in the ...to 2,3-linked sialic acid is required for gene transfer. The Journal of Biological Chemistry, 276(23),...

  17. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...careers?    Will you be encouraged to develop transferable skills by attending career events or being involved...resources on the Addgene blog Check out our transferable skills guide Learn about careers in science ...

  18. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...rabbits (Sandoval-Villegas et al., 2021).  Gene transfer Transposons are an alternative to viral vector...(mutagenesis screens, transgenic animals, gene transfer). The discovery of RNA guided transposons in bacteria...

  19. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...accurate tagging. In Fluorescence Resonance Energy Transfer (FRET) applications, the dyes need to have compatible...and emission profiles to enable effective energy transfer, which is key to observing interactions and conformational...
Showing: 201 - 220 of 363 results