We narrowed to 230 results for: pET

1. Plasmids 101: Gateway Cloning

   Type

   Blog Post

   Published

   Jan. 12, 2017, 3:30 p.m.

   ... are performed, the next step is to transform competent E. coli cells and select the positive clones. ...

2. AAV Titers: Where Do They Come From And What Do They Mean?

   Type

   Blog Post

   Published

   Nov. 15, 2017, 3 p.m.

   ...linear region of the assay. This may be due to competition for binding sites between the primers and the...

3. Lab to Office Culture Shock

   Type

   Blog Post

   Published

   Feb. 5, 2019, 1:04 p.m.

   ...Finally, if you still find the idea of not holding a pipette again upsetting, there are ways to get back to ...

4. CRISPR-mediated Plant Base Editors

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   Blog Post

   Published

   Jan. 3, 2019, 1:35 p.m.

   ...C) installation by base editors. Pathways that compete with the desired outcome are not pictured. ...

5. Viral Vectors 101: Pseudotyping

   Type

   Blog Post

   Published

   April 20, 2021, 2:30 p.m.

   ... CR, Chu HY, Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol...

6. Getting the Most from Your Lentiviral Transduction

   Type

   Blog Post

   Published

   April 7, 2016, 2:30 p.m.

   ...ml before the media becomes cloudy. Mycoplasma compete with host cells for nutrients and can alter expression...

7. Plasmids 101: E. coli Strains for Protein Expression

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   Blog Post

   Published

   Feb. 10, 2015, 3:06 p.m.

   ...Tetracycline RecA-deficient; best for plasmids with repetative sequences.  F- ompT lon hsdSB (rB- mB-) gal ...

8. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

   Type

   Blog Post

   Published

   May 13, 2025, 1:15 p.m.

   ...the production of lentiviral vectors that are competent for one round of infection (AKA transduction in...

9. Tips for Getting a Faculty Position

   Type

   Blog Post

   Published

   July 20, 2023, 1:15 p.m.

   ...for a Faculty Position" eBook Here 1. Have a competitive CV You don't necessarily need a Nature/Cell/Science...

10. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type

    Blog Post

    Published

    Sept. 1, 2020, 1:15 p.m.

    ... expression. References: Allocca M, Doria M, Petrillo M, Colella P, Garcia-Hoyos M, Gibbs D, Kim SR,...

11. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type

    Blog Post

    Published

    April 9, 2019, 1:13 p.m.

    ... PMC4927352. Janetopoulos, Chris, Tian Jin, and Peter Devreotes. "Receptor-mediated activation of heterotrimeric...

12. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type

    Blog Post

    Published

    July 10, 2018, 1:08 p.m.

    ...of time and effort and give the lab a serious competitive advantage.  Many thanks to our guest blogger...

13. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type

    Blog Post

    Published

    Sept. 5, 2017, 1:58 p.m.

    ...mutation. Here, the blocking-only templates will compete with the blocking/intended mutation templates resulting...

14. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type

    Blog Post

    Published

    March 5, 2019, 1:08 p.m.

    ...Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells...

15. Using Phosphoserine to Study Protein Phosphorylation

    Type

    Blog Post

    Published

    June 23, 2016, 2:30 p.m.

    ... E127-E130. PubMed PMID: 11988757. 2. Hornbeck, Peter V., et al. "PhosphoSitePlus, 2014: mutations, PTMs...

16. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type

    Blog Post

    Published

    April 24, 2015, 2:39 p.m.

    ...Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells...

17. Generating Mouse Models Using CRISPR/Cas9

    Type

    Blog Post

    Published

    July 12, 2016, 2:30 p.m.

    ...including 129 and C57BL/6, for which we have germline competent ESC lines. Second, the process is much quicker...

18. PiggyBac-ing Through the Genome Editing Field

    Type

    Blog Post

    Published

    May 31, 2016, 3:30 p.m.

    ...transposase (PBx), which contains mutations making it competent for excision, but defective for integration [4...

19. Finding Your Perfect Job After University

    Type

    Blog Post

    Published

    Jan. 12, 2016, 3:30 p.m.

    ...obtaining work in scientific research would be very competitive, and so I applied for a summer role as a Research...

20. Plasmids 101: Using Transposons in the Lab

    Type

    Blog Post

    Published

    July 6, 2021, 1:28 p.m.

    ... is a transposon? DNA transposons are mobile, repetitive genetic elements that are found in both prokaryotic...