We narrowed to 230 results for: pET

1. Transferable Skills Guide: External Collaborations

   Type

   Blog Post

   Published

   April 19, 2018, 1:25 p.m.

   ...But, as so often happens, one gets better with repetition. I learned to look at the event from the company...

2. AAV Titers: Where Do They Come From And What Do They Mean?

   Type

   Blog Post

   Published

   Nov. 15, 2017, 3 p.m.

   ...linear region of the assay. This may be due to competition for binding sites between the primers and the...

3. Plasmids 101: Stringent Regulation of Replication

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   Blog Post

   Published

   June 20, 2024, 1:15 p.m.

   ...number control of IncIalpha plasmid ColIb-P9 by competition between pseudoknot formation and antisense RNA...

4. The PAM Requirement and Expanding CRISPR Beyond SpCas9

   Type

   Blog Post

   Published

   Aug. 20, 2020, 2:30 p.m.

   ...Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung JK (2015) Engineered...

5. Hot Plasmids - August 2020

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   Blog Post

   Published

   Aug. 18, 2020, 1:15 p.m.

   ...plasmids were expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction. To biotinylate...

6. Getting the Most from Your Lentiviral Transduction

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   Blog Post

   Published

   April 7, 2016, 2:30 p.m.

   ...ml before the media becomes cloudy. Mycoplasma compete with host cells for nutrients and can alter expression...

7. Plasmids 101: E. coli Strains for Protein Expression

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   Blog Post

   Published

   Feb. 10, 2015, 3:06 p.m.

   ...Tetracycline RecA-deficient; best for plasmids with repetative sequences.  F- ompT lon hsdSB (rB- mB-) gal ...

8. Replacing Paper: Tips for Choosing an Electronic lab Notebook

   Type

   Blog Post

   Published

   July 10, 2018, 1:08 p.m.

   ...of time and effort and give the lab a serious competitive advantage.  Many thanks to our guest blogger...

9. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

   Type

   Blog Post

   Published

   April 9, 2019, 1:13 p.m.

   ... PMC4927352. Janetopoulos, Chris, Tian Jin, and Peter Devreotes. "Receptor-mediated activation of heterotrimeric...

10. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type

    Blog Post

    Published

    March 5, 2019, 1:08 p.m.

    ...Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells...

11. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type

    Blog Post

    Published

    Sept. 5, 2017, 1:58 p.m.

    ...mutation. Here, the blocking-only templates will compete with the blocking/intended mutation templates resulting...

12. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type

    Blog Post

    Published

    May 13, 2025, 1:15 p.m.

    ...the production of lentiviral vectors that are competent for one round of infection (AKA transduction in...

13. Tips for Getting a Faculty Position

    Type

    Blog Post

    Published

    July 20, 2023, 1:15 p.m.

    ...for a Faculty Position" eBook Here 1. Have a competitive CV You don't necessarily need a Nature/Cell/Science...

14. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type

    Blog Post

    Published

    Sept. 1, 2020, 1:15 p.m.

    ... expression. References: Allocca M, Doria M, Petrillo M, Colella P, Garcia-Hoyos M, Gibbs D, Kim SR,...

15. Using Phosphoserine to Study Protein Phosphorylation

    Type

    Blog Post

    Published

    June 23, 2016, 2:30 p.m.

    ... E127-E130. PubMed PMID: 11988757. 2. Hornbeck, Peter V., et al. "PhosphoSitePlus, 2014: mutations, PTMs...

16. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type

    Blog Post

    Published

    April 24, 2015, 2:39 p.m.

    ...Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells...

17. Generating Mouse Models Using CRISPR/Cas9

    Type

    Blog Post

    Published

    July 12, 2016, 2:30 p.m.

    ...including 129 and C57BL/6, for which we have germline competent ESC lines. Second, the process is much quicker...

18. PiggyBac-ing Through the Genome Editing Field

    Type

    Blog Post

    Published

    May 31, 2016, 3:30 p.m.

    ...transposase (PBx), which contains mutations making it competent for excision, but defective for integration [4...

19. Finding Your Perfect Job After University

    Type

    Blog Post

    Published

    Jan. 12, 2016, 3:30 p.m.

    ...obtaining work in scientific research would be very competitive, and so I applied for a summer role as a Research...

20. Plasmids 101: Using Transposons in the Lab

    Type

    Blog Post

    Published

    July 6, 2021, 1:28 p.m.

    ... is a transposon? DNA transposons are mobile, repetitive genetic elements that are found in both prokaryotic...