We narrowed to 461 results for: pac

...promoters, has limited the number of gRNAs that can be packaged into viral vectors for potential in vivo applications...therapeutic potential of the DAP array, the Gao Lab packaged MBE and MPE elements for deliveries via adeno-...

...can be limited by the need for a specific PAM (protospacer adjacent motif) in order to bind the target sites...These loops not only provide a 3-D structure for packing genomic DNA into nuclei, but are also thought to...

...FACS. In an additional approach, plasmids such as pACBSR-hyg and pFLP-hyg will be used to create gene deletions...biomarkers, and recently, cancer research related to space medicine. Now he aims on tracking pollution in rivers...

...lentivirus is not always an easy process. The cloning capacity of lentiviral vectors (~8.5 kb of insert) and ...plasmid DNA that you can use along with lentiviral packaging plasmids to create your own lentivirus stocks....

...and novelty in scientific research and in any workspace. Re-design lab meetings with a focus on discussion...making lab meetings more interactive and creating space for discussion at the end of each talk - time for...

...over their favorite science topics. What better space to do that than in the Addgene blog?  Fig...expertise of our antibody team to rely on. High-impact content like the antibody guide always gets an ...

...why hot dogs are sold in packages of 8 yet hot dog buns are sold in packages of 10…)  If you missed the...

... of performing similarly to Cas9 screens in a compacted library. Overall, Cas12a’s ability to target multiple...significant off-target effects, yet small enough to be packaged in AAV. A dCasMini-mediated adenine base editor...

...of the antibody - the paratopes - may change and impact the specificity and avidity of the antibodies produced... chain or light chain genes within a hybridoma impacts the specificity, avidity, and number of different...

...the use of alt text to describe images in online spaces. Alt text is used by screen readers to provide ...professional development workshop, “Make a Greater Impact Using Clear and Accessible Scientific Writing.”...

...a gRNA is useless without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases. The resource...the protein would be helpful to know for viral packaging limitations. Maybe you want to read literature...

...with some of our most popular biosensors already packaged and ready to deliver. Ready to give biosensors...AAV as your vehicle, we’ve got you covered with packaging plasmids! References and Resources References...

...tricky to find: each of the factors can negatively impact both translation and fidelity of translation, leading.... Or, to quote Ryan Mehl, PhD, GCE has “10x the impact of PCR. But it’s 100x more complicated.” And Ryan...

... these amazing tools in your experiments? Let’s unpack the myriad number of emerging viral applications...just one at a time. Many of these libraries are packaged and delivered with viral vectors instead of a ...

...the work you've done. If you want to maximize the impact of your communication, you will also have to explain...communicate with non-scientists, to establish broader impacts for their work in education and policy and to draw...

... about and develop technologies that will have impacts on many different facets of life. It also seems...neuroscience. AAV tools from Addgene are already having an impact on the field Not to toot our own horn too much,...

... life cycles bacterial DNA can be accidentally packaged into the newly created phages. Transfer of this...bacteriophage and plasmid properties. The phagemids are packaged into replication-incompetent phage particles with...

...offices, Addgene would not be able to have such an impact on scientific materials sharing. I would also like...further fulfilling our mission for an even bigger impact. In early 2016 we surveyed hundreds of scientists...

...targeted mutagenesis to generate hyper accurate Cas9 (HypaCas9). This nuclease showed decreased or similar off-target...Engineered CRISPR-Cas9 nuclease with expanded targeting space." Science 361.6408 (2018): 1259-1262. PubMed PMID...

...scientists know the results, your work won’t have any impact! There are lots of ways scientists communicate...efforts, inspire future research, and increase their impact. Open access and transparency Another major benefit...