We narrowed to 273 results for: plat

1. What's New in CRISPR - November 2021

   Type

   Blog Post

   Published

   Nov. 18, 2021, 2:15 p.m.

   ...other prime editing tools, the desired edits are templated by the 3′ extension of the pegRNAs. PEDAR is capable...

2. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

   Type

   Blog Post

   Published

   April 23, 2018, 2 p.m.

   ... electroporated cells re-adhered to the culture plate within 4 hr of treatment. In some primary cell lines...

3. RANbodies: Reporter Nanobody Fusions

   Type

   Blog Post

   Published

   April 10, 2018, 12:56 p.m.

   ...individual labs to complete. The nanobody based RANbody platform from the Sanes Lab overcomes this limitation and...

4. RaPID Detection of RNA-protein Interactions

   Type

   Blog Post

   Published

   Aug. 16, 2018, 12:40 p.m.

   ...interactions with RaPID RaPID is similar to the BioID platform for identifying protein-protein interactions, ...

5. dTAG - You're it!

   Type

   Blog Post

   Published

   June 21, 2018, 2:06 p.m.

   ...FKBP12F36V-tagged proteins using our lentiviral expression platform, to assess selective degradation of FKBP12F36V-BRD4...

6. Seven Tips for Using LinkedIn as a Scientist

   Type

   Blog Post

   Published

   Oct. 1, 2019, 1:21 p.m.

   ...volunteer organizational work. LinkedIn is not the platform for politics, family updates, or click bait of...

7. Working with Nuclear Receptors

   Type

   Blog Post

   Published

   July 15, 2014, 4:03 p.m.

   ... The development of the transient transfection platform for assaying transcriptional activity, for example...

8. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   Published

   Aug. 29, 2024, 1:15 p.m.

   ...cloning, perform an exonuclease cleanup, transform, plate, and pick. In short, the Fragmid system takes the...

9. Plasmids 101: Modular Cloning

   Type

   Blog Post

   Published

   March 12, 2024, 1:15 p.m.

   ... them to these organisms via another optimized platform. Gene Stacking As the name implies, gene stacking...

10. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ...Other” column. For example, you may enter “donor template vector”. Cloning Method Choose from: Gateway...

11. Hot Plasmids and Viral Preps - March 2021

    Type

    Blog Post

    Published

    March 23, 2021, 1:15 p.m.

    ...assembly By Eric Perkins Though it’s easy enough to contemplate DNA in terms of its sequencing, in vivo studies...

12. Early Career Researcher Toolbox: Finding Relevant Papers

    Type

    Blog Post

    Published

    Jan. 9, 2020, 1:45 p.m.

    ...the topic.  Meta Meta is a literature discovery platform developed by the Chan Zuckerberg Initiative. Meta...

13. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    Published

    July 24, 2018, 12:51 p.m.

    ...Consortium, also known as the Genetic Perturbation Platform, to produce their shRNA library to support functional...

14. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    Published

    June 26, 2018, 1:33 p.m.

    ... of Glycosylation (APCDG) and an international platform for CDG research, named the CDG&Allies – Professionals...

15. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type

    Blog Post

    Published

    May 22, 2018, 1:38 p.m.

    ... MET genes to grow.) Students then use replica plating to determine if expression of MET/Met genes from...

16. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type

    Blog Post

    Published

    Oct. 6, 2020, 1:15 p.m.

    ... WA (2013) Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression...

17. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    Published

    July 14, 2016, 2:30 p.m.

    ...mechanism. To prevent new editing post-HDR, repair templates should remove the PAM sequence. Many research ...

18. Plasmids 101: Terminators and PolyA signals

    Type

    Blog Post

    Published

    March 31, 2016, 2:30 p.m.

    ...100% efficient at halting transcription of the template and initiating the desired cleavage event, although...

19. Production of Virus in Insect Versus Mammalian Cells

    Type

    Blog Post

    Published

    Nov. 5, 2024, 8:32 p.m.

    ... S., & Ng, S. K. (2021). HEK293 cell line as a platform to produce recombinant proteins and viral vectors...

20. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type

    Blog Post

    Published

    June 25, 2024, 1:15 p.m.

    ...primer binding site and a reverse transcription template (RTT). The design of the pegRNA has been shown...