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Showing: 201 - 220 of 515 results

  1. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ... also disrupted to elucidate their role in the process. The results of these studies led scientists to...sequence. Separate Cas proteins then express and process the CRISPR loci to generate RNA, which guide a ...

  2. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...and eukaryotes, which use a binary replication process whereby one cell copies itself and “splits” into...” genome with genes central to the replication process removed. This means the vector can deliver a genetic...

  3. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ...samples and visualize. For more details on this procedure, find the protocol here.   Tips and troubleshooting...Ultrafast tissue staining with chemical tags." Proceedings of the National Academy of Sciences111.36 (2014...

  4. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    .... Created withBioRender.com.   Biological Processes  If you’re feeling relieved that you’re working...visualize an abstract protein during your sample prep process, imagining how it starts and if any steps could...

  5. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...intramolecular DNA recombination. This naturally occurring process is mediated by recombinases like RecA and RecBCD...analysis of in vitro intramolecular recombination. Proceedings of the National Academy of Sciences 77:4847–4851...

  6. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...their resumes have a definite advantage in our process. We see candidates who have done the following ...still be practicing and getting familiar with the process. Bonus points for being able to just look at a ...

  7. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...DNA Cloning: A Personal View after 40 Years.”Proceedings of the National Academy of Sciences of the United...Biologically Functional Bacterial Plasmids In Vitro.” Proceedings of the National Academy of Sciences of the United...

  8. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...plays a pivotal role in an array of biological processes including development, the immune system, and ...cell turnover. Apoptosis is a highly controlled process that is triggered by internal and external signals...

  9. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...and how these arrangements may affect nuclear processes. The latest of these visualization tools is CRISPR-Sirius...labeling of chromosomal loci in human cells.” Proceedings of the National Academy of Sciences of the United...

  10. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...to be incorporated into a host cell (through a process called transformation), essentially giving the ...shelf-stable until water is added and protein production proceeds. FD-CF pellets are easy for students to use in...

  11. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ... tested in clinical trials. It is speeding the process of the discovery/development of genetic targets...off-target effects is a major limitation. The procedure will also need to be perfected until the efficiency...

  12. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...fragments containing the desired mutations. This same process can be used simultaneously for the generation of... multiple cloning steps. You can simplify this process by using NEBuilder which requires only a single-stranded...

  15. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ... start studying up before you begin the hiring process. Read Genevive Bjorn's "Ready, Set, Hire" on Nature...home message Starting a lab can be a challenging process. Each PI emphasizes the importance of slowly building...

  16. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...material (such as plasmids) into mammalian cells, a process called transfection. Transfection is somewhat comparable.... Since the DNA introduced in the transfection process is not integrated into the nuclear genome, in the...

  17. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...intensity. If your samples and controls were processed the same way, the GFP- population should overlap...samples and control underwent different treatment processes, you may see a shift in the negative population...

  18. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...factors as proxies for a cellular process or directly measure a cellular process. Cellular phenomena such as...

  19. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ... the nick site, which was expected since the processivity window of PolI is 15-20 bp. EvolvR’s on-target...bacteriophage T7 DNA polymerase increased the processivity of PolI3M, and thereby increasing the editing...
Showing: 201 - 220 of 515 results