...with transgene-free induced pluripotent stem cells. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83...somatic cells using a single polycistronic vector. Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62. ... temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A. 2012 May 29. Palecek Fibroblasts...conversion of human fibroblasts to dopaminergic neurons. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10343-8...self-renewing, tripotent neural precursor cells. Proc Natl Acad Sci U S A. 2012 Feb 14;109(7):2527-32....

... Site-Specific Recombination in Transgenic Mice. Proc Natl Acad Sci. Jul 15;89(14):6232-6. PubMed . Matsuda...Transgenes Introduced by In Vivo Electroporation. Proc Natl Acad Sci. Jan 16;104(3):1027-32. doi: 10.1073...Cells by the Cre Recombinase of Bacteriophage P1. Proc Natl Acad Sci. Jul;85(14):5166-70. PubMed . Sternberg...Cre-lox-Regulated Conditional RNA Interference from Transgenes. Proc Natl Acad Sci. Jul 13;101(28):10380-5. doi:10.1073...Mammalian Capecchi 126006 pAAV2-ProC3-Cre/mCherry Cre and mCherry ProC3 AAV Roska 126700 pHAGE2-EF1aL-Cre-IRES-NeoR-W...floxed portion of a construct during the cloning process). VCre and SCre are alternative Cre recombinases...the stop codon is excised, and gene expression proceeds. Cre-dependent Gene Knockout: Conversely, putting...

...improved genetically encoded fluorescent sensor. Proc Natl Acad Sci U S A. 2004 Dec 14;101(50):17404-9...and Golgi Zn2+ with genetically encoded sensors. Proc Natl Acad Sci U S A. 2011 May 3;108(18):7351-6. ... cells revealed by single GFP-linked biosensors. Proc Natl Acad Sci U S A. 2008 Jan 8. Wolfgang Dostmann...high-frequency release at Schaffer collateral synapses. Proc Natl Acad Sci U S A. 2018 May 7. pii: 1720648115...in muscles and neurons of Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2019 Aug 1. Alexander Gottschalk..., the activity of specific genes and cellular processes, or other factors like environmental conditions...largely preserving spatial and temporal cellular processes. Note that many of the articles linked below include...

...understand its function and pathology. Mohanty et al. Proc Natl Acad Sci U S A. 2023 Aug 22. Use a lentiviral...disease and implicate a wide range of cellular processes. The vast majority of cases (90-95%) of ALS are...al. Cell Rep Methods. 2022 Mar 28. Follow APP processing and dynamics using APP and Bace1 plasmids . Capitini...

...MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16589-94...

...CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A . 112(10):3002-7. PMID: 25713381...arrays. Separate Cas proteins then express and process the CRISPR loci to generate CRISPR RNAs (crRNAs...-CRISPR RNA (pre-crRNA). (2) The pre-crRNA is processed into individual crRNAs by a special trans-activating...non-homologous end joining (NHEJ) , an error-prone process that produces insertions/deletions disrupting a...a DSB at the target genomic locus. Repair will proceed through NHEJ or HDR depending on the experimental...

...quality control (QC) processes to screen for and reduce these errors. These processes allow us to have a...developed a process for identifying, categorizing, and resolving a variety of QC issues. This process ensures...discrepancies. While Addgene’s QC process adds time to the deposit process, we believe this to be non-negotiable...research, these errors can affect experimental processes, results, and subsequent conclusions. Being vigilant... edit, and easy to share. However, the cloning process — including the propagation of plasmids in bacteria... [email protected] to get started! Addgene’s QC process Addgene prioritizes the sequence quality of the...provided, each deposit goes through the same QC process to ensure all plasmids pass our high-quality standards...

...We’ve talked a lot about the quality control process at Addgene by introducing our new sequencing partner...regularly use Snapgene for our quality control process because of its expansive feature library and useful...Identifying plasmid features While our quality control process for incoming plasmids can vary based on the contents...

... issues.  The permanent residence “green card” process is a necessary and often overlooked part of career...start planning for your visa and or green card process well before addressing your career alternatives... benefit from the applicant thinking about the process early, keeping good records, and planning for the...organizations do not have the April 1 H-1B queue process or quota like industry, these organizations can...achievements. Participating in the scientific review process as a reviewer as proof of your judging the work...You can still assist your PI in the peer review process, but remember you cannot use it as documentary ...that you can take to prepare for the green card process Update your CV and bio sketch whenever you have...

...genomes. Nern A, Pfeiffer BD, Svoboda K, Rubin GM. Proc Natl Acad Sci USA. 2011 Aug 23;108(34):14198-203...Allak B, van Drunen E, Kanaar R, Berns A, Jonkers J. Proc Natl Acad Sci USA. 2001 Jul 31; 98(16): 9209–9214...

...enhance magnetic cancer cell capture. Fierer et al (Proc Natl Acad Sci U S A. 2014 Mar 17. PubMed) Peptide...through engineering a bacterial adhesin. Zakeri et al (Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7....protein-peptide interactions. In a study published in Proceedings of the National Academy of Sciences in March,...SpyLigase system for the first time? Howarth: The Proceedings of the National Academy of Sciences paper [published...

... gene expression: Implications for gene therapy. Proc Natl Acad Sci U S A, 95(19), 11377. https://doi....Undergoing Spontaneous Degeneration in Tissue Culture. Proc Soc Exp Biol Med. 84(3), 570–573. https://doi.org...encode proteins essential to initiating the whole process – such as the viral DNA polymerase – it makes sense...

... “Ligand-Regulated Site-Specific Recombination.” Proc. Natl. Acad. Sci. USA 92(June): 5940–44. PubMed ...Using a Ligand-Dependent Chimeric Cre Recombinase.” Proc Natl Acad Sci U S A 92(15): 6991–95. PubMed PMID...

...mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5547-51.... hybrid derived from the trp and lac promoters.” Proc Natl Acad Sci U S A. 80(1) (1983): 21-5. PubMed ...over the TetO elements, allowing transcription to proceed (pJKR-H-tetR from the Church lab uses this system...Response Element (TRE)), allowing transcription to proceed. When tetracycline or one of its derivatives is...linked in groups of 6), allowing transcription to proceed. When cumate is added, it binds cTA, resulting ...

...piggyBac transposase tools for genome engineering.,” Proc. Natl. Acad. Sci. U. S. A., vol. 110, no. 25, pp...provides a simple tool for conditional cell ablation.,” Proc. Natl. Acad. Sci. U. S. A., vol. 105, no. 38, pp...to transfect, select, and isolate clones. This procedure is cumbersome and inefficient, as it relies on...incorporated, and used during the differentiation process. Moreover, a splinkerette-PCR assay may be used...

...Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7....

...topoisomerase I in Escherichia coli is sequence specific."  Proc Natl Acad Sci U S A.  1991 Nov 15;88(22):10104-8...restriction enzymes or ligase, and requires no post-PCR procedures. Sounds easy right? The technique relies on the...insert, the magic of TOPO cloning happens. Basic procedure Let’s break down the steps needed for TOPO cloning... everything you need for the next step before proceeding. Pre-warming your antibiotic-containing plate...

...genomics of biotechnologically important yeasts. Proc. Natl. Acad. Sci., 113, 9882-9887. DOI: 10.1073/...

... for real-time multicolor luminescence imaging.” Proc Natl Acad Sci U S A 112(14) (2015): 4352-6. PMID...activation, you can’t use fluorescence to monitor processes directly affected by light. Long-term light exposure...